A Review on Microbial Enzymes in the Food Industry: Current Innovations and Future Prospects

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Year : 2026 | Volume : 15 | 01 | Page :
    By

    Rajesh Kumar,

  • Chanchal,

  • Baanipreet Kaur,

  • Ruby Deshwal,

  • Ghazal Firoz,

  • Anjali Bharti,

  • Vinod Kumar Gupta,

  1. Student, Rapture Biotech International (P) Ltd., Noida, Uttar Pradesh, India
  2. Student, Rapture Biotech International (P) Ltd., Noida, Uttar Pradesh, India
  3. Student, Rapture Biotech International (P) Ltd., Noida, Uttar Pradesh, India
  4. Student, Rapture Biotech International (P) Ltd., Noida, Uttar Pradesh, India
  5. Student, Rapture Biotech International (P) Ltd., Noida, Uttar Pradesh, India
  6. Student, Rapture Biotech International (P) Ltd., Noida, Uttar Pradesh, India
  7. Chief Scientific Officer, Rapture Biotech International (P) Ltd., Noida, Uttar Pradesh, India

Abstract

Over the past few decades, microbial enzymes have become indispensable biocatalysts in food production, fundamentally revolutionizing how we approach manufacturing processes while simultaneously enhancing the quality of what we produce. Today, approximately 200 of the 4,000 known enzymes are deployed commercially with proteases, amylases, lipases, and lactases leading the charge in industrial food applications. This review brings together the latest innovations in how we use microbial enzymes for food processing, while also exploring exciting possibilities for their future. These enzymes do more than just improve texture and flavor, they extend shelf life, minimize waste, and enable the creation of genuinely sustainable food products. What makes this particularly exciting is how enzyme engineering, genetic modification, and precision fermentation have opened entirely new possibilities for customizing enzyme properties to meet specific industrial needs. The global food enzymes market continues to experience remarkable growth, fueled by what consumers increasingly demand: natural processing aids, products with clean labels, and manufacturing approaches that respect environmental constraints. But the field still faces real hurdles like regulatory compliance, maintaining enzyme stability under challenging industrial conditions and the persistent challenge of production costs all remain significant obstacles. Throughout this review, we examine where microbial enzymes make the biggest impact across dairy, bakery, beverages, meat processing, and specialty food sectors, while also addressing regulatory frameworks and charting out where enzyme technology development might head next.

Keywords: : Microbial enzymes, food processing, amylase, protease, lipase, enzyme engineering, sustainable food production, food quality, enzyme biotechnology, industrial applications

How to cite this article:
Rajesh Kumar, Chanchal, Baanipreet Kaur, Ruby Deshwal, Ghazal Firoz, Anjali Bharti, Vinod Kumar Gupta. A Review on Microbial Enzymes in the Food Industry: Current Innovations and Future Prospects. Research & Reviews : Journal of Food Science & Technology. 2026; 15(01):-.
How to cite this URL:
Rajesh Kumar, Chanchal, Baanipreet Kaur, Ruby Deshwal, Ghazal Firoz, Anjali Bharti, Vinod Kumar Gupta. A Review on Microbial Enzymes in the Food Industry: Current Innovations and Future Prospects. Research & Reviews : Journal of Food Science & Technology. 2026; 15(01):-. Available from: https://journals.stmjournals.com/rrjofst/article=2026/view=236234


References

1. Bell EL, Finnigan W, France SP, Green AP, Hayes MA, Hepworth LJ, Lovelock SL, Niikura H, Osuna S, Romero E, Ryan KS. Biocatalysis. Nature Reviews Methods Primers. 2021 Jun 24;1(1):46.Available from: http://dx.doi.org/10.1038/s43586-021-00044-z
2. Siddiqui SA, Erol Z, Rugji J, Taşçı F, Kahraman HA, Toppi V, Musa L, Di Giacinto G, Bahmid NA, Mehdizadeh M, Castro-Muñoz R. An overview of fermentation in the food industry-looking back from a new perspective. Bioresources and bioprocessing. 2023 Nov 28;10(1):85.Available from: http://dx.doi.org/10.1186/s40643-023-00702-y
3. Singh R, Kumar M, Mittal A, Mehta PK. Microbial enzymes: industrial progress in 21st century. 3 Biotech. 2016 Dec;6(2):174.Available from: http://dx.doi.org/10.1007/s13205-016-0485-8
4. Mascarin GM, Golo PS, de Souza Ribeiro-Silva C, Muniz ER, de Oliveira Franco A, Kobori NN, et al. Advances in submerged liquid fermentation and formulation of entomopathogenic fungi. Appl Microbiol Biotechnol.2024;108(1):451. Available from: http://dx.doi.org/10.1007/s00253-024-13287-z
5. Boukid F, Ganeshan S, Wang Y, Tülbek MÇ, Nickerson MT. Bioengineered enzymes and precision fermentation in the food industry. Int J Mol Sci . 2023;24(12):10156. Available from: http://dx.doi.org/10.3390/ijms241210156
6. Olempska-Beer ZS, Merker RI, Ditto MD, DiNovi MJ. Food-processing enzymes from recombinant microorganisms–a review. Regul Toxicol Pharmacol.2006;45(2):144–58. Available from: http://dx.doi.org/10.1016/j.yrtph.2006.05.001
7. Rebholz GF, Sebald K, Dirndorfer S, Dawid C, Hofmann T, Scherf KA. Impact of exogenous α-amylases on sugar formation in straight dough wheat bread. Eur Food Res Technol. 2021;247(3):695–706. Available from: http://dx.doi.org/10.1007/s00217-020-03657-y
8. Espinosa-Ramírez J, Pérez-Carrillo E, Serna-Saldívar SO. Maltose and glucose utilization during fermentation of barley and sorghum lager beers as affected by β-amylase or amyloglucosidase addition. J Cereal Sci. 2014;60(3):602–9. Available from: http://dx.doi.org/10.1016/j.jcs.2014.07.008
9. Zhao X, Zheng Z, Zhang J, Sarwar A, Aziz T, Yang Z. Change of proteolysis and sensory profile during ripening of Cheddar-style cheese as influenced by a microbial rennet from rice wine. Food Sci Nutr.2019;7(4):1540–50. Available from: http://dx. doi: 10.1002/fsn3.1003
10. Madhusankha GDMP, Thilakarathna RCN. Meat tenderization mechanism and the impact of plant exogenous proteases: A review. Arab J Chem. 2021;14(2):102967. Available from: http://dx.doi.org/10.1016/j.arabjc.2020.102967
11. Gu M, Hong T, Ma Y, Xi J, Zhao Q, Xu D, et al. Effects of a commercial peptidase on rheology, microstructure, gluten properties of wheat dough and bread quality. Lebenson WissTechnol.2022;160(113266):113266.Availablefrom:http://dx.doi.org/10.1016/j.lwt.2022.113266
12. McSweeney PLH, Sousa MJ. Biochemical pathways for the production of flavour compounds in cheeses during ripening: A review. Le Lait. 2000 May;80(3):293–324. Available from: https://doi.org/10.1051/lait:2000127
13. Reyes-Reyes AL, Valero Barranco F, Sandoval G. Recent advances in lipases and their applications in the food and nutraceutical industry. Catalysts. 2022;12(9):960. Available from: http://dx.doi.org/10.3390/catal12090960
14. Sivakanthan S, Madhujith T. Current trends in applications of enzymatic interesterification of fats and oils: A review. Lebenson Wiss Technol . 2020;132(109880):109880. Available from: http://dx.doi.org/10.1016/j.lwt.2020.109880
15. Bendelja Ljoljić D, Dolenčić Špehar I, Kos I, Hulak N, Vnučec I. Lactose-free dairy products: A growing market and nutritional advantages. In: SBORNÍK L KONFERENCE O JAKOSTI POTRAVIN A POTRAVINOVÝCH SUROVIN / BOOK OF THE 50th FOOD QUALITY AND SAFETY CONFERENCE. Mendel University in Brno; 2024. p. 41–5. Available from: https://doi.org/10.11118/978-80-7509-996-9-0041
16. Ribeiro DS, Henrique SMB, Oliveira LS, Macedo GA, Fleuri LF. Enzymes in juice processing: a review: Enzymes in juice processing. Int J Food Sci Technol. 2010;45(4):635–41. Available from: http://dx.doi.org/10.1111/j.1365-2621.2010.02177.x
17. Liu X, Wang C, Zhang X, Zhang G, Zhou J, Chen J. Application prospect of protein-glutaminase in the development of plant-based protein foods. Foods. 2022;11(3):440. Available from: http://dx.doi.org/10.3390/foods11030440
18. Li L, Pei Y, Cheng K, Deng Y, Dong X, Fang R, et al. Production and evaluation of enzyme-modified cheese adding protease or lipase to improve quality properties. J Biosci Bioeng. 2023;135(5):389–94. Available from: http://dx.doi.org/10.1016/j.jbiosc.2023.02.006
19. Marcellino N, Beuvier E, Grappin R, Guéguen M, Benson DR. Diversity of Geotrichum candidum strains isolated from traditional cheesemaking fabrications in France. Appl Environ Microbiol. 2001;67(10):4752–9. Available from: http://dx.doi.org/10.1128/aem.67.10.4752-4759.2001
20. Yin L, Wei Y, Wu W, Peng Y, Ding W, Xiao S, et al. Effect of enzymes on baking quality of wheat dough and storage quality of toast bread. Int J Food Sci Technol. 2024;59(12):9479–89. Available from: http://dx.doi.org/10.1111/ijfs.17597
21. Decamps K, Joye IJ, Courtin CM, Delcour JA. Glucose and pyranose oxidase improve bread dough stability. J Cereal Sci.2012;55(3):380–4. Available from: http://dx.doi.org/10.1016/j.jcs.2012.01.007
22. Evans E. Brewing: science and practice. DE Briggs, CA Boulton, PA Brookes and R Stevens Woodhead Publishing, Cambridge UK/CRC Press, Boca Raton, Florida, USA, 2004. 881 pp ISBN 0‐8493‐2547‐1. J Sci Food Agric.2006;86(1):169–169. Available from: http://dx.doi.org/10.1002/jsfa.2344
23. Stanek-Wandzel N, Krzyszowska A, Zarębska M, Gębura K, Wasilewski T, Hordyjewicz-Baran Z, et al. Evaluation of cellulase, pectinase, and hemicellulase effectiveness in extraction of phenolic compounds from grape pomace. Int J Mol Sci. 2024;25(24):13538. Available from: http://dx.doi.org/10.3390/ijms252413538
24. Hailemichael F. Production and industrial application of microbial aspartic protease: A review. International Journal of Food Engineering and Technology [Internet]. 2021;5(2):85. Available from: http://dx.doi.org/10.11648/j.ijfet.20210502.17
25. González-Osuna MF, Bernal-Mercado AT, Wong-Corral FJ, Ezquerra-Brauer JM, Soto-Valdez H, Castillo A, et al. Bioactive peptides and protein hydrolysates used in meat and meat products’ Preservation─A review. ACS Food Sci Technol. 2024;4(5):1003–16. Available from: http://dx.doi.org/10.1021/acsfoodscitech.3c00605
26. Fan Y, Zheng S, Annamalai PK, Bhandari B, Prakash S. Enhancement of the texture and microstructure of faba bean-based meat analogues with brewers’ spent grain through enzymatic treatments. Sustain Food Technol. 2024;2(3):826–36. Available from: http://dx.doi.org/10.1039/d4fb00045e
27. Ye H, Tao X, Zhang W, Chen Y, Yu Q, Xie J. Food-derived bioactive peptides: production, biological activities, opportunities and challenges. Journal of Future Foods. 2022;2(4):294–306. Available from: http://dx.doi.org/10.1016/j.jfutfo.2022.08.002
28. Porter JL, Rusli RA, Ollis DL. Directed evolution of enzymes for industrial biocatalysis. Chembiochem [Internet]. 2016;17(3):197–203. Available from: http://dx.doi.org/10.1002/cbic.201500280
29. Hu Z, Liu Y, Huang Y, Yu P. Advances in the directed evolution of computer-aided enzymes. Curr Top Med Chem [Internet]. 2025;26. Available from: http://dx.doi.org/10.2174/0115680266377310250526045137
30. Deckers M, Deforce D, Fraiture M-A, Roosens NHC. Genetically modified micro-organisms for industrial food enzyme production: An overview. Foods [Internet]. 2020;9(3):326. Available from: http://dx.doi.org/10.3390/foods9030326
31. Huang W, Wang S, Wei Y, Bai Y, Zhu Z, Yin D, et al. Design and evolution of artificial enzyme with in-situ biosynthesized non-canonical amino acid. Nat Commun [Internet]. 2025;16(1):8698. Available from: http://dx.doi.org/10.1038/s41467-025-63733-3
32. Hu J, Arantes V, Pribowo A, Saddler JN. The synergistic action of accessory enzymes enhances the hydrolytic potential of a “cellulase mixture” but is highly substrate specific. Biotechnol Biofuels [Internet]. 2013;6(1):112. Available from: http://dx.doi.org/10.1186/1754-6834-6-112
33. Nutrition C for FS and A. Guidance for Industry: Recommendations for Submission of Chemical and Technological Data for Food Additive Petitions and GRAS Notices for Enzyme Preparations [Internet]. U.S. Food and Drug Administration. 2022. Available from: https://www.fda.gov/regulatory-information/search-fda-guidance-documents/guidance-industry-recommendations-submission-chemical-and-technological-data-food-additive-petitions
34. Zhang Y, Geary T, Simpson BK. Genetically modified food enzymes: a review. Curr Opin Food Sci [Internet]. 2019;25:14–8. Available from: http://dx.doi.org/10.1016/j.cofs.2019.01.002
35. Modarres HP, Mofrad MR, Sanati-Nezhad A. Protein thermostability engineering. RSC Adv [Internet]. 2016;6(116):115252–70. Available from: http://dx.doi.org/10.1039/c6ra16992a
36. Du Y-H, Wang M-Y, Yang L-H, Tong L-L, Guo D-S, Ji X-J. Optimization and scale-up of fermentation processes driven by models. Bioengineering (Basel) [Internet]. 2022;9(9):473. Available from: http://dx.doi.org/10.3390/bioengineering9090473
37. Hwang H, Nam S-J. The influence of consumers’ knowledge on their responses to genetically modified foods. GM Crops Food [Internet]. 2021;12(1):146–57. Available from: http://dx.doi.org/10.1080/21645698.2020.1840911
38. Søndergaard HA, Grunert KG, Scholderer J. Consumer attitudes to enzymes in food production. Trends Food Sci Technol [Internet]. 2005;16(10):466–74. Available from: http://dx.doi.org/10.1016/j.tifs.2005.06.003
39. Khan MF. Recent advances in microbial enzyme applications for sustainable textile processing and waste management. Sci [Internet]. 2025;7(2):46. Available from: http://dx.doi.org/10.3390/sci7020046
40. Prithviraj V, Díaz LP, Lemus-Mondaca R, Ullah A, Roopesh MS. Emerging advancements in 3D food printing. Front Food Sci Technol [Internet]. 2025;5. Available from: http://dx.doi.org/10.3389/frfst.2025.1607449

Ahead of Print Subscription Review Article
Volume 15
01
Received 10/01/2025
Accepted 16/12/2025
Published 22/01/2026
Publication Time 377 Days
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