Adaptive Mechanisms of Rumen Microbiota to High-Fiber Diets: Mystery of Fiber Degradation and Nutrient Utilization for Improved Host Performance

Year : 2026 | Volume : 15 | Issue : 01 | Page : 7 17
    By

    Md. Emran Hossain,

  1. Professor, Department of Animal Science and Nutrition, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram, Bangladesh

Abstract

The rumen microbiota plays a critical role in the digestion of plant fibers, enabling herbivores to derive essential nutrients from fibrous feed sources. The adaptation of these microbial communities to high-fiber diets is crucial for optimizing fiber degradation and nutrient utilization. This study explores the mechanisms by which rumen microbes adjust to handle the complex structure of fibrous materials such as cellulose, hemicellulose, and lignin. Through an integrated approach, we examine key processes such as the upregulation of cellulolytic and fibrolytic bacteria, enzymatic production, shifts in microbial diversity, and the role of fungi in breaking down lignocellulosic compounds. Additionally, we explore microbial interactions, such as cross-feeding between fiber-degrading bacteria and methanogens, and how these symbiotic relationships enhance fermentation efficiency. The study highlights how microbial biofilm formation and buffering compounds protect against the acidic by-products of fermentation, supporting sustained microbial activity. Moreover, we investigate microbial–host interactions, focusing on how the rumen microbiota influences nutrient absorption and overall host performance. Understanding these adaptive mechanisms provides valuable insights into improving livestock nutrition, particularly under conditions of high-fiber feeding. By enhancing fiber fermentation, these microbial adaptations contribute to improved energy production, optimal nutrient absorption, and overall animal performance, offering potential strategies for more efficient and sustainable livestock management.

Keywords: Adaptation, biofilm formation, cellulolytic bacteria, enzymatic activity, fiber degradation, microbial diversity, microbial–host interaction

[This article belongs to Research and Reviews : Journal of Dairy Science and Technology ]

How to cite this article:
Md. Emran Hossain. Adaptive Mechanisms of Rumen Microbiota to High-Fiber Diets: Mystery of Fiber Degradation and Nutrient Utilization for Improved Host Performance. Research and Reviews : Journal of Dairy Science and Technology. 2026; 15(01):7-17.
How to cite this URL:
Md. Emran Hossain. Adaptive Mechanisms of Rumen Microbiota to High-Fiber Diets: Mystery of Fiber Degradation and Nutrient Utilization for Improved Host Performance. Research and Reviews : Journal of Dairy Science and Technology. 2026; 15(01):7-17. Available from: https://journals.stmjournals.com/rrjodst/article=2026/view=243609


References

  1. Shi H, Guo P, Wang Z, Zhou J, He M, Shi L, et al. Cellulase enhancing rumen microbiome of Tan sheep indicates plastic responses to seasonal variations of diet in the typical steppe. BMC Microbiol. 2025 Mar 18;25(1):154. doi: 1186/s12866-025-03799-7.
  2. Pu G, Hou L, Zhao Q, Liu G, Wang Z, Zhou W, et al. Interactions between gut microbes and host promote degradation of various fiber components in Meishan pigs. mSystems. 2025 Feb 18;10(2):
    e01500–24. doi: 1128/msystems.01500-24.
  3. Li T, Raja BR, Liao J, Zheng L, Yin F, Gan S, et al. The characteristics, influence factors, and regulatory strategies of growth retardation in ruminants: A review. Front Vet Sci. 2025 Mar 26;12:1566427. doi: 3389/fvets.2025.1566427.
  4. Wang F, Xie B, Ji H, Xia J, Hao Y, Cao Z, et al. Temporal modulation of duodenal microbiota in dairy cows: Effects of dietary shift from high forage to high concentration. Front Vet Sci. 2025 Apr 4;12:1551327. doi: 3389/fvets.2025 .1551327.
  5. Wang Y, Yin X, Hao K, Wang C, Wei W, Li Y, et al. Consumption of steam explosion and fermentation-pretreated corn stover affects the growth performance of sheep by shifting the rumen microbiota community structure. Front Microbiol. 2025 Apr 3;16:1532746. doi: 3389/fmicb.2025.1532746.
  6. Wang S, Kong F, Dai D, Li C, Hao Y, Wang E, et al. Deterministic succession patterns in the rumen and fecal microbiome associate with host metabolic shifts in peripartum dairy cattle. GigaScience. 2025;14:giaf042. doi: 1093/gigascience /giaf042.
  7. Fu R, Han L, Li Q, Li Z, Dai Y, Leng J. Studies on the concerted interaction of microbes in the gastrointestinal tract of ruminants on lignocellulose and its degradation mechanism. Frontiers in Microbiology. 2025 May 9;16:1554271. [Online]. Available: https://pmc.ncbi.nlm.nih.gov/articles/
    PMC12098361/.
  8. Li B, Jia G, Wen D, Zhao X, Zhang J, Xu Q, et al. Rumen microbiota of indigenous and introduced ruminants and their adaptation to the Qinghai–Tibetan plateau. Front Microbiol. 2022 Oct 10;13:
    doi: 10.3389/fmicb.2022.1027138.
  9. Zhu W, Chang L, Shi S, Lu N, Du S, Li J, et al. Gut microbiota reflect adaptation of cave-dwelling tadpoles to resource scarcity. ISME J. 2024 Jan 1;18(1):wrad009. doi: 1093/ismejo/wrad009.
  10. Chen Q, Sha Y, Liu X, He Y, Chen X, Yang W, et al. Unique rumen micromorphology and microbiota–metabolite interactions: Features and strategies for Tibetan sheep adaptation to the plateau. Front Microbiol. 2024 Oct 9;15:1471732. doi: 3389/fmicb.2024.1471732.
  11. Hu B, Wang J, Li Y, Ge J, Pan J, Li G, et al. Gut microbiota facilitates adaptation of the plateau zokor (Myospalax baileyi) to the plateau living environment. Front Microbiol. 2023 Feb 24;14:
    doi: 10.3389/fmicb.2023.1136845.
  12. Zhao J, Yao Y, Dong M, Xiao H, Xiong Y, Yang S, et al. Diet and high altitude strongly drive convergent adaptation of gut microbiota in wild macaques, humans, and dogs to high altitude environments. Front Microbiol. 2023 Feb 23;14:1067240. doi: 3389/fmicb.2023 .1067240.
  13. Chang S, Ma Y, Cheng Y, Kong X, Li H, Li C, et al. Mannan oligosaccharides enhance rumen cellulose degradation by modulating microbial composition and glycoside hydrolase expression. J Biotech Res. 2024;19:230–41 . Available: https://www.btsjournals.com/assets/2024v19p230-241.pdf
  14. Liang Y, Wang F, Jia R, Li J, Wang L, Li Y, et al. Cellulase activity and age-based variation of intestinal microbiota in Hezuo pigs. Front Microbiol. 2025 May 9;16:1599847. doi: 3389/fmicb.2025.1599847.
  15. Weimer PJ, Waghorn GC, Odt CL, Mertens DR. Effect of diet on populations of three species of ruminal cellulolytic bacteria in lactating dairy cows. J Dairy Sci. 1999;82(1):122–34. doi: 3168 /jds.S0022-0302(99)75216-1.
  16. Zhang Y, Wang Y, Wanyan R, Yao B, Tan Z, Wang R, et al. The influence of multi-generational high-fiber diet on the gut microbiota of root voles (Microtus oeconomus). 2024 Aug. doi: 10.21203/rs.3.rs-4858686/v1.
  17. Ran T, Saleem AM, Shen Y, Ribeiro GO Jr, Beauchemin KA, Tsang A, et al. Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance, and total tract digestibility of heifers fed a high forage diet. J Anim Sci. 2019 Jul 30;97(8):3578–87. doi: 1093/jas/skz216.
  18. Miccoli FE, Pérez CD, Vargas-Bello-Perez E, Danelón JL, Cantet JM, Martínez R, et al. Effects of high fiber energy supplements on production performance, milk composition and milk fatty acid profile from dairy ewes fed fresh cut Lolium multiflorum. Small Rumin Res. 2022 Apr 1;209:106640. doi: 1016/j.smallrumres.2022.106640.
  19. Le Sciellour M, Labussière E, Zemb O, Renaudeau D. Effect of dietary fiber content on nutrient digestibility and fecal microbiota composition in growing-finishing pigs. PLoS One. 2018 Oct 24;13(10):e0206159. doi: 1371/journal .pone.0206159.
  20. Baniel A, Amato KR, Beehner JC, Bergman TJ, Mercer A, Perlman RF, et al. Seasonal shifts in the gut microbiome indicate plastic responses to diet in wild geladas. Microbiome. 2021 Jan 23;9(1):26. doi: 1186/s40168-020-00977-9.
  21. Zhao X, Zhang Y, Rahman A, Chen M, Li N, Wu T, et al. Rumen microbiota succession throughout the perinatal period and its association with postpartum production traits in dairy cows: A review. Anim Nutr. 2024 Sep 1;18:17–26. doi: 1016/j.aninu.2024.04.013.
  22. Li K, Shi B, Na R. The Colonization of Rumen Microbiota and Intervention in Pre-Weaned Ruminants. Animals. 2023;13(6). doi: 3390/ani13060994.
  23. Hu C, Ding L, Jiang C, Ma C, Liu B, Li D, et al. Effects of management, dietary intake, and genotype on rumen morphology, fermentation, and microbiota, and on meat quality in yaks and cattle. Front Nutr. 2021 Nov 11;8:755255. doi: 3389/fnut.2021.755255.
  24. Fan Q, Cui X, Wang Z, Chang S, Wanapat M, Yan T, et al. Rumen microbiota of Tibetan sheep (Ovis aries) adaptation to extremely cold season on the Qinghai-Tibetan Plateau. Front Vet Sci. 2021 May 25;8:673822. doi: 3389/fvets.2021.673822.
  25. Zou H, Li Q, Liu J, Wang X, Gao Q, Yang Y, et al. Fecal microbiota reveal adaptation of herbivores to the extreme environment of the Qinghai–Tibet Plateau. Grassl Res. 2024 Jun;3(2):155–70. doi: 1002/glr2.12075.
  26. Wang Y, Zhou R, Yu Q, Feng T, Li H. Gut microbiome adaptation to extreme cold winter in wild plateau pika (Ochotona curzoniae) on the Qinghai-Tibet Plateau. FEMS Microbiol Lett. 2020 Aug;367(16):fnaa134. doi: 1093/femsle/fnaa134.
  27. Xiao K, Liang X, Lu H, Li X, Zhang Z, Lu X, et al. Adaptation of gut microbiome and host metabolic systems to lignocellulosic degradation in bamboo rats. ISME J. 2022 Aug;16(8):1980–92. doi: 1038/s41396-022-01247-2.
  28. Yan XT, Yan BY, Ren QM, Dou JJ, Wang WW, Zhang JJ, et al. Effect of slow-release urea on the composition of ruminal bacteria and fungi communities in yak. Anim Feed Sci Technol. 2018 Oct 1;244:18–27. doi: 1016/j.anifeedsci.2018 .07.016.
  29. Liang Z, Zhang J, Ahmad AA, Han J, Gharechahi J, Du M, et al. Forage lignocellulose is an important factor in driving the seasonal dynamics of rumen anaerobic fungi in grazing yak and cattle. Microbiol Spectr. 2023 Oct 17;11(5):e00788–23. doi: 1128 /spectrum.00788-23.
  30. Xue F, Nan X, Sun F, Pan X, Guo Y, Jiang L, et al. Metagenome sequencing to analyze the impacts of thiamine supplementation on ruminal fungi in dairy cows fed high-concentrate diets. AMB Express. 2018 Oct 3;8(1):159. doi: 1186/s13568-018-0680-6.
  31. Langda S, Zhang C, Zhang K, Gui B, Ji D, Deji C, et al. Diversity and composition of rumen bacteria, fungi, and protozoa in goats and sheep living in the same high-altitude pasture. Animals (Basel). 2020 Jan 22;10(2):186. doi: 3390/ani10020186.
  32. Li M, Zi X, Yang H, Ji F, Tang J, Lv R, et al. Effects of king grass and sugarcane top in the absence or presence of exogenous enzymes on the growth performance and rumen microbiota diversity of goats. Trop Anim Health Prod. 2021 Mar;53(1):106. doi: 1007/s11250-020-02544-8.
  33. Kala A, Kamra DN, Kumar A, Agarwal N, Chaudhary LC, Joshi CG. Impact of levels of total digestible nutrients on microbiome, enzyme profile and degradation of feeds in buffalo rumen. PLoS One. 2017 Feb 16;12(2):e0172051. doi: 1371/journal.pone.0172051.
  34. Song SD, Chen GJ, Guo CH, Rao KQ, Gao YH, Peng ZL, et al. Effects of exogenous fibrolytic enzyme supplementation to diets with different NFC/NDF ratios on the growth performance, nutrient digestibility and ruminal fermentation in Chinese domesticated black goats. Anim Feed Sci Technol. 2018 Feb 1;236:170–7. doi: 1016/j .anifeedsci.2017.12.008.
  35. Phungviwatnikul T, Lee AH, Belchik SE, Suchodolski JS, Swanson KS. Weight loss and high-protein, high-fiber diet consumption impact blood metabolite profiles, body composition, voluntary physical activity, fecal microbiota, and fecal metabolites of adult dogs. J Anim Sci. 2022;100(2). doi: 1093/jas/skab379.
  36. Wang Y, Zhao Y, Tang X, Nan X, Jiang L, Wang H, et al. Nutrition, gastrointestinal microorganisms and metabolites in mastitis occurrence and control. Anim Nutr. 2024 Jun 1;17:220-31. doi: 1016/j.aninu.2024.01.010.
  37. Liu M, Zhang Y, Liu Y, Li Y, Wang Z, Ge G, et al. Effect of fermented total mixed rations on rumen microbial communities and serum metabolites in lambs. Grassl Res. 2024 Sep;3(3):249–63. doi: 1002/glr2.12095.
  38. Vargas JE, López-Ferreras L, Andrés S, Mateos I, Horst EH, López S. Differential diet and pH effects on ruminal microbiota, fermentation pattern and fatty acid hydrogenation in RUSITEC continuous cultures. Fermentation. 2023 Mar 23;9(4):320. doi: 3390/fermentation9040320.
  39. Xu H, Wang G, Gao Q, Liu Z, Jia J, Xu Y, et al. Microbial insights into ruminal fiber degradation and feed efficiency of Hu sheep. Front Microbiol. 2025 Apr 22;16:1561336. doi: 3389/fmicb.
    2025.1561336.
  40. Justice KE, Smith FA. A model of dietary fiber utilization by small mammalian herbivores, with empirical results for Neotoma. Am Nat. 1992;139(2):398–416. doi: 1086/285333.
  41. Wu J, Yang D, Gong H, Qi Y, Sun H, Liu Y, et al. Multiple omics analysis reveals that high fiber diets promote gluconeogenesis and inhibit glycolysis in muscle. BMC Genomics. 2020 Sep 24;21(1):660. doi: 1186/s12864-020-07048-1.
  42. Gao Q, Sun G, Duan J, Luo C, Yangji C, Zhong R, et al. Alterations in gut microbiota improve SCFA production and fiber utilization in Tibetan pigs fed alfalfa diet. Front Microbiol. 2022 Oct 21;13:969524. doi: 10.3389/fmicb.2022.969524.
  43. Zhou Z, Zhou X, Li J, Zhong Z, Li W, Liu X, et al. Transcriptional regulation and adaptation to a high-fiber environment in Bacillus subtilis HH2 isolated from feces of the giant panda. PLoS One. 2015;10(2):e0116935. doi: 10.1371/journal.pone.0116935.
  44. Elghandour MM, Tan ZL, Abu Hafsa SH, Adegbeye MJ, Greiner R, Ugbogu EA, et al. Saccharomyces cerevisiae as a probiotic feed additive to non and pseudo-ruminant feeding: A review. J Appl Microbiol. 2020;128(3):658–674. doi: 1111/jam.14416.
  45. Jouany JP, Medina B, Bertin G, Julliand V. Effect of live yeast culture supplementation on hindgut microbial communities and their polysaccharidase and glycoside hydrolase activities in horses fed a high-fiber or high-starch diet. J Anim Sci. 2009;87(9):2844–2852. doi: 2527/jas.2008-1602.
  46. Patel V, Patel AK, Parmar NR, Patel AB, Reddy B, Joshi CG. Characterization of the rumen microbiome of Indian Kankrej cattle (Bos indicus) adapted to different forage diet. Appl Microbiol Biotechnol. 2014;98(23):9749–9761. doi: 1007/s00253-014-6153-1.
  47. Bian G, Yu S, Cheng C, Huang H, Liu J. Ruminal microbiota-host crosstalks promote ruminal epithelial development in neonatal lambs with alfalfa hay introduction. mSystems. 2024;9(2). doi: 1128/msystems.01034-23.
  48. Hou L, Ma Y, Li J, Yang Y, Duan P, Guo T. Effects of dietary supplementation with different amounts of Lycium ruthenicum (black goji berry) branch roughage on plasma biochemical indices and rumen microflora of sheep. Front Microbiol. 2025 Apr 8;16:1556724. doi: 3389/fmicb.2025.1556724.
  49. Gao Z, Liu B, La S, Li D, Zhu X, Sun H, et al. Alfalfa hay substitution for wheat straw improves beef quality via rumen microflora alteration. Heliyon. 2023 Oct 1;9(10). doi: 1016/j.heliyon.2023.e20803.
  50. Coates LC, Storms D, Finley JW, Fukagawa NK, Lemay DG, Kalscheur KF, et al. A low-starch and high-fiber diet intervention impacts the microbial community of raw bovine milk. Curr Dev Nutr. 2022 Jun 1;6(6):nzac086. doi: 1093/cdn/nzac086.
  51. Cui X, Wang Z, Guo P, Li F, Chang S, Yan T, et al. Shift of feeding strategies from grazing to different forage feeds reshapes the rumen microbiota to improve the ability of Tibetan sheep (Ovis aries) to adapt to the cold season. Microbiol Spectr. 2023;11(2). doi: 1128/spectrum.02816-22.
  52. Marques RD, Cooke RF. Effects of ionophores on ruminal function of beef cattle. Animals (Basel). 2021 Sep 30;11(10):2871. doi: 3390/ani11102871.
  53. Hu R, Zou H, Wang H, Wang Z, Wang X, Ma J, et al. Dietary energy levels affect rumen bacterial populations that influence the intramuscular fat fatty acids of fattening yaks (Bos grunniens). Animals (Basel). 2020 Aug 22;10(9):1474. doi: 3390/ani10091474.
  54. Yang S, Zheng J, Mao H, Vinitchaikul P, Wu D, Chai J. Multiomics of yaks reveals significant contribution of microbiome into host metabolism. npj Biofilms Microbiomes. 2024 Nov 21;10(1):133. doi: 10.1038/s41522-024-00609-2.
  55. Li Q, Fei HL, Luo ZH, Gao SM, Wang PD, Lan LY, et al. Gut microbiome responds compositionally and functionally to the seasonal diet variations in wild gibbons. npj Biofilms Microbiomes. 2023 Apr 21;9(1):21. doi: 1038/s41522-023-00388-2.
  56. Zhao J, Yao Y, Li D, Zhu W, Xiao H, Xie M, et al. Metagenome and metabolome insights into the energy compensation and exogenous toxin degradation of gut microbiota in high-altitude rhesus macaques (Macaca mulatta). npj Biofilms Microbiomes. 2023 Apr 20;9(1):20. doi: 1038/s41522-023-00387-3.
  57. Zhao MA, Chu J, Feng S, Guo C, Xue B, He K, et al. Immunological mechanisms of inflammatory diseases caused by gut microbiota dysbiosis: A review. Biomed Pharmacother. 2023 Aug 1;164:114985. doi: 1016/j.biopha.2023.114985.
  58. Wen Y, Li S, Wang Z, Feng H, Yao X, Liu M, et al. Intestinal microbial diversity of free-range and captive yak in Qinghai Province. Microorganisms. 2022 Mar 31;10(4):754. doi: 3390/
    microorganisms10040754.
  59. Keum GB, Pandey S, Kim ES, Doo H, Kwak J, Ryu S, et al. Understanding the Diversity and Roles of the Ruminal Microbiome. J Microbiol. 2024;62(3):217–230. doi: 1007/s12275-024-00121-4.
  60. Yan L, Tang L, Zhou Z, Lu W, Wang B, Sun Z, et al. Metagenomics reveals contrasting energy utilization efficiencies of captive and wild camels (Camelus ferus). Integr Zool. 2022;17(3):333–345. doi: 1111/1749-4877.12585.
  61. Mu Y, Qi W, Zhang T, Zhang J, Mao S. Multi-omics analysis revealed coordinated responses of rumen microbiome and epithelium to high-grain-induced subacute rumen acidosis in lactating dairy cows. mSystems. 2022;7(1). doi: 1128/msystems.01490-21.
  62. Jia T, Zhang W, Zhu W, Fan L. Intermittent fasting driven different adaptive strategies in Eothenomys miletus (red-backed vole) at different altitudes: based on the patterns of variations in intestinal microbiota. BMC Microbiol. 2025 Mar 31;25(1):185. doi: 1186/s12866-025-03934-4.
  63. Wang K, Ren A, Zheng M, Jiao J, Yan Q, Zhou C, et al. Diet with a high proportion of rice alters profiles and potential function of digesta-associated microbiota in the ileum of goats. Animals (Basel). 2020 Jul 24;10(8):1261. doi: 3390/ani10081261.
  64. Fan G, Su N, He Y, Yuan C, Zhao C, Hu X, et al. Carbonate buffer mixture alleviates subacute rumen acidosis induced by long-term high-concentrate feeding in dairy goats by regulating rumen microbiota. Microorganisms. 2025 Apr 19;13(4):945. doi: 3390/microorganisms13040945.
  65. Wang X, Guo C, Xu X, Zhang L, Li S, Dai D, et al. Effect of Alkaline Mineral Complex Buffer Supplementation on Rumen Fermentation, Rumen Microbiota and Rumen Epithelial Transcriptome of Newborn Calves. Fermentation. 2023;9(11). doi: 3390/fermentation9110973.
  66. Elmhadi ME, Ali DK, Khogali MK, Wang H. Subacute ruminal acidosis in dairy herds: Microbiological and nutritional causes, consequences, and prevention strategies. Anim Nutr. 2022;10:148–155. doi: 1016/j.aninu.2021.12.008.
  67. Metzler BU, Mosenthin R. A review of interactions between dietary fiber and the gastrointestinal microbiota and their consequences on intestinal phosphorus metabolism in growing pigs. Asian-Australas J Anim Sci. 2008;21(4):603–615. doi: 5713/ajas.2008.r.03.
  68. Li QS, Wang R, Ma ZY, Zhang XM, Jiao JZ, Zhang ZG, et al. Dietary selection of metabolically distinct microorganisms drives hydrogen metabolism in ruminants. ISME J. 2022;16(11):2535–2546. doi: 1038/s41396-022-01294-9.
  69. Yang R, Guo Y, Zhang S, Hao Q, Duan C, Wang Y, et al. Effect of Dioscorea opposite waste supplementation on antioxidant capacity, immune response and rumen microbiome in weaned lambs. Fermentation. 2023 Mar 4;9(3):256. doi: 3390 /fermentation9030256.
  70. Zhong Z, Sun P, Zhang Y, Li L, Han D, Pan X, et al. Differential responses of rumen and fecal fermentation and microbiota of Liaoning cashmere goats after 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester supplementation. Sci Rep. 2024 Apr 12;14(1):8505. doi: 1038/s41598-024-58581-y.
  71. Na SW. Understanding the role of rumen epithelial host-microbe interactions in cattle feed efficiency. Anim Nutr. 2022 Sep 1;10:41–53. doi: 1016/j.aninu.2022.04.002.
Regular Issue Subscription Review Article
Volume 15
Issue 01
Received 30/11/2025
Accepted 03/03/2026
Published 06/03/2026
Publication Time 96 Days
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