RP-HPLC method for simultaneous determination of Drospirenone and Ethinylestradiol in Bulk and their Pharmaceutical dosage form


Year : 2024 | Volume : 11 | Issue : 03 | Page : 46-67
    By

    Vipul T Prajapati,

  • Shantilal P Padhiyar,

  • Carol P Macwan,

  • Tejal G Soni,

  • B N Suhagia,

  • Rohan V. Chauhan,

  1. Assistant Professor, Department of Pharmaceutical Chemistry, Dharmsinh Desai University, Nadiad, Gujarat, India
  2. Assistant Professor, Department of Pharmaceutical Chemistry, Dharmsinh Desai University, Nadiad, Gujarat, India
  3. Assistant Professor, Department of Pharmacognosy, Dharmsinh Desai University, Nadiad, Gujarat, India
  4. Dean, Dharmsinh Desai University, Nadiad, Gujarat, India
  5. Professor, Faculty of Pharmacy, Dharmsinh Desai University, Nadiad, Gujarat, India
  6. Assistant Professor, Department of Pharmaceutical Chemistry, Dharmsinh Desai University, Nadiad, Gujarat, India

Abstract

Oral contraceptive is used to prevent the pregnancy which is contain hormones to block the release of eggs from the ovaries. Most of them contain estrogen and progesterone. Nowadays, usage of hormonal contraceptive is sharply enhancing; around 100 million of women worldwide are taking these drugs. With this drug fertility rate can be supressed as long as they want or plan their activity along with these drugs provide efficacy, reliability, low cost and overall safety of oral contraceptive. Currently, oral contraceptive is available in parenteral formulation. Contraceptive has two types: 1) Female contraceptive 2) Male contraceptive. Drospirenone and Ethylestradiol is decreasing follicle stimulating hormone (FSH) and luteinizing (LH) for preventing pregnancy by inhibiting ovulation. There isn’t a published green method for RP-HPLC analysis of DRSP (drospirenone) and ETH (ethinylestradiol) that uses green analytical chemistry (GAC). The application of GAC in the creation and verification of a green RP-HPLC method is the main topic of the current work. Green solvents, specifically acetonitrile and acetate buffer (60:40, v/v, pH 6.0), were utilised as the mobile phase in RP-PLC. At 277 nm, the detection was done. The linearity, range, precision, accuracy, limit of detection, limit of quantification, sensitivity, and specificity of the method were all validated. It was shown to be linear in the range of 10- 50 μg/mL for ETH and 300-1500 μg/mL for DRSP. In comparison to existing documented approaches, our approach showed exceptional environmental friendliness. To lessen the possible risks that organic solvents may have to the environment, the pharmaceutical industry should investigate these newly developed environmentally friendly green solutions.

Keywords: Oral contraceptive, FSH, LH, RP-HPLC, extreme greenness, method validation.

[This article belongs to Trends in Drug Delivery (tdd)]

How to cite this article:
Vipul T Prajapati, Shantilal P Padhiyar, Carol P Macwan, Tejal G Soni, B N Suhagia, Rohan V. Chauhan. RP-HPLC method for simultaneous determination of Drospirenone and Ethinylestradiol in Bulk and their Pharmaceutical dosage form. Trends in Drug Delivery. 2024; 11(03):46-67.
How to cite this URL:
Vipul T Prajapati, Shantilal P Padhiyar, Carol P Macwan, Tejal G Soni, B N Suhagia, Rohan V. Chauhan. RP-HPLC method for simultaneous determination of Drospirenone and Ethinylestradiol in Bulk and their Pharmaceutical dosage form. Trends in Drug Delivery. 2024; 11(03):46-67. Available from: https://journals.stmjournals.com/tdd/article=2024/view=180580


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Regular Issue Subscription Review Article
Volume 11
Issue 03
Received 03/09/2024
Accepted 25/10/2024
Published 30/10/2024