First Insights into Whole Genome Sequencing of the Mycobacterium tuberculosis Complex: Molecular Diversity and Drug Susceptibility Patterns in Senegal

Year : 2025 | Volume : 02 | Issue : 01 | Page : –
    By

    Abdou Padane,

  • Seyni Ndiaye,

  • Malle Gueye,

  • Mamadou Nda,

  • Marièma Sarr,

  • Aminata Mboup,

  • Marilyne Aza-Gnandji,

  • Darrel Ornelle Elion Assiana,

  • Ndèye Astou Dabo,

  • Abdoulaye Leye Sarr,

  • Yacine Amet Dia,

  • Mame Sokhna Gueye,

  • Ndeye Ndack Cissé,

  • Bineta Diouf,

  • Joséphine Khady Badiane,

  • Papa Alassane Diaw,

  • Abdoulaye Samba Diallo,

  • Ousmane Diop,

  • Ousmane Diouf,

  • Raghavendra Amachawadi,

  • Fatoumata Ba,

  • Yacine Mar Diop,

  • Souleymane Mboup,

  1. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Senegal
  2. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Senegal
  3. Researcher, Programme National de lutte contre la Tuberculose (PNT), Dakar, Senegal
  4. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Senegal
  5. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Senegal
  6. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Senegal
  7. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Senegal
  8. Laboratory Assistant, Laboratoire National de Référence des Mycobactéries (LNRM) du Programme National de lutte contre la Tuberculose (PNT), Brazzaville, Congo
  9. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Senegal
  10. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Senegal
  11. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Senegal
  12. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Senegal
  13. Researcher, Programme National de lutte contre la Tuberculose (PNT), Dakar, Sénégal
  14. Researcher, Programme National de lutte contre la Tuberculose (PNT), Dakar, Sénégal
  15. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF)Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Sénégal
  16. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF)Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Sénégal
  17. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF)Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Sénégal
  18. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF)Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Sénégal
  19. Pharmacist Researcher, Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF)Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Sénégal
  20. Researcher, Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, USA
  21. Researcher, Programme National de lutte contre la Tuberculose (PNT), Dakar, Sénégal
  22. Researcher, Programme National de lutte contre la Tuberculose (PNT), Dakar, Sénégal
  23. Pharmacist Researcher, Institute de Recherche en Santé, de Surveillance Épidémiologique et de Formation (IRESSEF), Dakar, Sénégal

Abstract

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Background: We conducted a bibliographic analysis of the Mycobacterium tuberculosis complex (MTBC) in sub-Saharan Africa, which included the analysis of 8,139 genomic sequences from 34 of the 49 sub-Saharan African countries. Notably, only one complete sequence from Senegal was identified, which had been generated in the United States. Our primary objective was to utilize whole genome sequencing (WGS) to detect resistance in anti-tuberculous strains of the MTBC in Senegal. This work seeks to advance the understanding of the molecular diversity and distribution of tuberculosis in Senegal. Methods: A prospective study was conducted in Senegal involving patients diagnosed with tuberculosis (TB). Genomic DNA was extracted from positive culture isolates and subjected to whole genome sequencing (WGS) using Illumina MiSeq technology. This approach enabled the characterization of Mycobacterium tuberculosis complex (MTBC) species and lineages, as well as the identification of mutations associated with treatment resistance. The findings confirmed the significant presence of MTBC and diverse lineages in Senegal. Results: Our results showed that all strains isolated from patients were Mycobacterium tuberculosis sensu stricto, with a predominance of Euro-American sub-lineages. Through bioinformatics analysis, we predicted resistance to 20 anti-tuberculosis (first and second line) drugs for each sample. In silico antibiogram analysis identified strains resistant to isoniazid and other multi- drug-resistant strains, with resistance associated mutations detected in the katG and rpoB genes. Conclusion: This study significantly contributed to the molecular surveillance of Mycobacterium tuberculosis complex in Senegal. The species identification of M. tuberculosis isolates is crucial for the rapid diagnosis of tuberculosis in patients, thereby facilitating improved clinical management and treatment outcomes.

Keywords: Mycobacterium tuberculosis complex, Lineage 4, Molecular distribution, Drug susceptibility test, Senegal.

[This article belongs to Recent Trends in Infectious Diseases ]

How to cite this article:
Abdou Padane, Seyni Ndiaye, Malle Gueye, Mamadou Nda, Marièma Sarr, Aminata Mboup, Marilyne Aza-Gnandji, Darrel Ornelle Elion Assiana, Ndèye Astou Dabo, Abdoulaye Leye Sarr, Yacine Amet Dia, Mame Sokhna Gueye, Ndeye Ndack Cissé, Bineta Diouf, Joséphine Khady Badiane, Papa Alassane Diaw, Abdoulaye Samba Diallo, Ousmane Diop, Ousmane Diouf, Raghavendra Amachawadi, Fatoumata Ba, Yacine Mar Diop, Souleymane Mboup. First Insights into Whole Genome Sequencing of the Mycobacterium tuberculosis Complex: Molecular Diversity and Drug Susceptibility Patterns in Senegal. Recent Trends in Infectious Diseases. 2025; 02(01):-.
How to cite this URL:
Abdou Padane, Seyni Ndiaye, Malle Gueye, Mamadou Nda, Marièma Sarr, Aminata Mboup, Marilyne Aza-Gnandji, Darrel Ornelle Elion Assiana, Ndèye Astou Dabo, Abdoulaye Leye Sarr, Yacine Amet Dia, Mame Sokhna Gueye, Ndeye Ndack Cissé, Bineta Diouf, Joséphine Khady Badiane, Papa Alassane Diaw, Abdoulaye Samba Diallo, Ousmane Diop, Ousmane Diouf, Raghavendra Amachawadi, Fatoumata Ba, Yacine Mar Diop, Souleymane Mboup. First Insights into Whole Genome Sequencing of the Mycobacterium tuberculosis Complex: Molecular Diversity and Drug Susceptibility Patterns in Senegal. Recent Trends in Infectious Diseases. 2025; 02(01):-. Available from: https://journals.stmjournals.com/rtid/article=2025/view=0


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Regular Issue Subscription Original Research
Volume 02
Issue 01
Received 24/01/2025
Accepted 13/02/2025
Published 17/02/2025
Publication Time 24 Days
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