Enhancing Rhizoremediation of Oil-Contaminated Soil Using Brachiaria ramosa: A Study on Plant Growth Promotion and Degradation Potential

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Year : 2026 | Volume : 16 | Issue : 01 | Page : 24 41
    By

    N. K. Madhumita,

  • Shellsiya M,

  • R. Nirmal Kumar,

  • D. S. Ranjith Santhosh Kumar,

  • P. Suganya,

  1. Student, PSGR Krishnammal College for Women, Coimbatore, Tamil Nadu, India
  2. Student, PSGR Krishnammal College for Women, Coimbatore, Tamil Nadu, India
  3. Head, Assistant Professor, PSGR Krishnammal College for Women, Coimbatore, Tamil Nadu, India
  4. Assistant Professor, PSGR Krishnammal College for Women, Coimbatore, Tamil Nadu, India
  5. Assistant Professor, PSGR Krishnammal College for Women, Coimbatore, Tamil Nadu, India

Abstract

Soil contamination by petroleum hydrocarbons is a serious problem for agriculture and the environment since it can result in ecological hazards, decreased productivity, and soil degradation. Remedial techniques that are both economical and sustainable are especially crucial in developing nations. To improve hydrocarbon breakdown, rhizoremediation has become a popular environmentally friendly method that takes advantage of the mutually beneficial relationships between plant roots and rhizospheric microorganisms. In addition to assessing Brachiachiaria ramosa’s potential as an efficient rhizoremediator, this research looks at how plant-growth-promoting rhizobacteria (PGPR) and hydrocarbon-degrading microbial consortia can enhance plant performance and speed up biodegradation. Along with elements impacting remediation effectiveness, such as soil physicochemical characteristics, microbial composition, and environmental circumstances, important mechanisms are covered, such as root exudation, microbial stimulation, and plant–microbe interactions. The limits that exist today and the potential for widespread use are also emphasized. A sustainable and profitable method for improving agricultural resilience and recovering hydrocarbon-contaminated soils is the integration of B. ramosa with effective microbial consortia.

Keywords: Rhizoremediation, Brachiaria ramosa, petroleum hydrocarbons, plant-growth-promoting rhizobacteria (PGPR), microbial consortia, soil remediation, sustainable agriculture, environmental restoration

[This article belongs to Research and Reviews : A Journal of Biotechnology ]

How to cite this article:
N. K. Madhumita, Shellsiya M, R. Nirmal Kumar, D. S. Ranjith Santhosh Kumar, P. Suganya. Enhancing Rhizoremediation of Oil-Contaminated Soil Using Brachiaria ramosa: A Study on Plant Growth Promotion and Degradation Potential. Research and Reviews : A Journal of Biotechnology. 2026; 16(01):24-41.
How to cite this URL:
N. K. Madhumita, Shellsiya M, R. Nirmal Kumar, D. S. Ranjith Santhosh Kumar, P. Suganya. Enhancing Rhizoremediation of Oil-Contaminated Soil Using Brachiaria ramosa: A Study on Plant Growth Promotion and Degradation Potential. Research and Reviews : A Journal of Biotechnology. 2026; 16(01):24-41. Available from: https://journals.stmjournals.com/rrjobt/article=2026/view=239981


References

  1. Abhigna D, Kalpana R, Radhamani S, Ravichandran V, Janaki P, Geetha P. Performance of brown top millet (Brachiaria ramosa L.) grown under problematic soils. J Appl Nat Sci. 2024;16(2):503–7. doi:10.31018/jans.v16i2.5471.
  2. Atlas RM, Hazen TC. Oil biodegradation and bioremediation: A tale of the two worst spills in U.S. history. Environ Sci Technol. 2011;45(16):6709–15. doi:10.1021/es2013227.
  3. Backer R, Rokem JS, Ilangumaran G, Lamont J, Praslickova D, Ricci E, et al. Plant growth-promoting rhizobacteria: Context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Front Plant Sci. 2018;9:1473. doi:10.3389/fpls.2018.01473.
  4. Barea JM, Pozo MJ, Azcón R, Azcón-Aguilar C. Microbial co-operation in the rhizosphere. J Exp Bot. 2005;56(417):1761–78. doi:10.1093/jxb/eri197.
  5. Bashan Y, de-Bashan LE, Prabhu SR, Hernandez JP. Advances in plant growth-promoting bacterial inoculant technology: Formulations and practical perspectives (1998–2013). Plant Soil. 2014;378(1–2):1–33. doi:10.1007/s11104-013-1956-x.
  6. Bento FM, Camargo FAO, Okeke BC, Frankenberger WT Jr. Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. Bioresour Technol. 2005;96(9):1049–55. doi:10.1016/j.biortech.2004.09.008.
  7. Das N, Chandran P. Microbial degradation of petroleum hydrocarbon contaminants: An overview. Biotechnol Res Int. 2011;2011:941810. doi:10.4061/2011/941810.
  8. Glick BR, Cheng Z, Czarny J, Duan J. Promotion of plant growth by ACC deaminase-producing soil bacteria. Eur J Plant Pathol. 2007;119(3):329–39. doi:10.1007/s10658-007-9162-4.
  9. Head IM, Jones DM, Röling WFM. Marine microorganisms make a meal of oil. Nat Rev Microbiol. 2006;4(3):173–82. doi:10.1038/nrmicro1348.
  10. Jansson JK, Hofmockel KS. Soil microbiomes and climate change. Nat Rev Microbiol. 2020;18(1):35–46. doi:10.1038/s41579-019-0265-7.
  11. Jørgensen KS, Puustinen J, Suortti AM. Bioremediation of petroleum hydrocarbon-contaminated soil by composting in biopiles. Environ Pollut. 2000;107(2):245–54. doi:10.1016/S0269-7491(99)00144-X.
  12. Kästner M, Breuer-Jammali M, Mahro B. Impact of inoculation protocols, salinity, and pH on degradation of PAHs and survival of introduced bacteria in soil. Appl Environ Microbiol. 1998;64(1):359–62. doi:10.1128/AEM.64.1.359-362.1998.
  13. Kong Z, Liu H. Modification of rhizosphere microbial communities: A possible mechanism of PGPR enhancing plant growth and fitness. Front Plant Sci. 2022;13:920813. doi:10.3389/fpls.2022.920813.
  14. Kuiper I, Lagendijk EL, Bloemberg GV, Lugtenberg BJJ. Rhizoremediation: A beneficial plant–microbe interaction. Mol Plant Microbe Interact. 2004;17(1):6–15. doi:10.1094/MPMI.2004.17.1.6.
  15. Lakshmi PM, Jaison S, Muthukumar T, Muthukumar M. Assessment of metal accumulation capacity of Brachiaria ramosa from cement waste area. Ecol Eng. 2013;60:96–8. doi:10.1016/j.ecoleng.2013.07.043.
  16. Liu D, Luan X, Guo J, Cui T, An J, Zheng R. A new approach of oil spill detection using time-resolved LIF. Sensors (Basel). 2016;16(9):1347. doi:10.3390/s16091347.
  17. Lugtenberg B, Kamilova F. Plant-growth-promoting rhizobacteria. Annu Rev Microbiol. 2009;63:541–56. doi:10.1146/annurev.micro.62.081307.162918.
  18. Majeed BK, Shwan DMS, Rashid KA. Environmental contamination of petroleum hydrocarbons: Effects and remediation approaches. Environ Sci Process Impacts. 2025;27(3):526–48. doi:10.1039/d4em00548a.
  19. Margesin R, Schinner F. Biodegradation and bioremediation of hydrocarbons in extreme environments. Appl Microbiol Biotechnol. 2001;56(5–6):650–63. doi:10.1007/s002530100701.
  20. Melzi A, Zecchin S, Gomarasca S, Abruzzese A, Cavalca L. Ecological indicators and resources for hydrocarbon rhizoremediation. Front Bioeng Biotechnol. 2024;12:1379947. doi:10.3389/fbioe.2024.1379947.
  21. Merkl N, Schultze-Kraft R, Infante C. Phytoremediation in the tropics—Effect of crude oil on plant growth. Bioremediat J. 2004;8(3–4):177–84. doi:10.1080/10889860490887527.
  22. Mekonnen BA, Aragaw TA, Genet MB. Bioremediation of petroleum hydrocarbon-contaminated soil: Principles and advancements. Front Environ Sci. 2024;12:1354422. doi:10.3389/fenvs.2024.1354422.
  23. Nannipieri P, Ascher J, Ceccherini MT, Landi L, Pietramellara G, Renella G. Microbial diversity and soil functions. Eur J Soil Sci. 2003;54(4):655–70. doi:10.1046/j.1351-0754.2003.0556.x.
  24. Oleńska E, Małek W, Wójcik M, Świecicka I, Thijs S, Vangronsveld J. Beneficial features of PGPR in challenging conditions: A review. Sci Total Environ. 2020;743:140682. doi:10.1016/j.scitotenv.2020.140682.
  25. Ossai IC, Ahmed A, Hassan A, Hamid FS. Remediation of petroleum hydrocarbon-contaminated soil and water: A review. Environ Technol Innov. 2020;17:100526. doi:10.1016/j.eti.2019.100526.
  26. Prince RC, Garrett RM, Bare RE, Grossman MJ, Townsend T, Suflita JM, et al. Roles of photooxidation and biodegradation in long-term weathering of oils. Spill Sci Technol Bull. 2003;8(2):145–56. doi:10.1016/S1353-2561(03)00017-3.
  27. Prince RC, Gramain A, McGenity TJ. Prokaryotic hydrocarbon degraders. In: Timmis KN, editor. Handbook of hydrocarbon and lipid microbiology. Berlin: Springer; 2010. p. 1669–92. doi:10.1007/978-3-540-77587-4_118.
  28. Semple KT, Morriss AWJ, Paton GI. Bioavailability of hydrophobic organic contaminants in soils. Eur J Soil Sci. 2003;54(4):809–18. doi:10.1046/j.1351-0754.2003.0564.x.
  29. Singh A, Yadav VK, Chundawat RS, Soltane R, Awwad NS, Ibrahium HA, et al. Enhancing PGPR activities through consortium exposure: A review. Front Bioeng Biotechnol. 2023;11:1099999. doi:10.3389/fbioe.2023.1099999.
  30. Tang JC, Wang RG, Niu XW, Wang M, Chu HR, Zhou QX. Characterisation of rhizoremediation of petroleum-contaminated soil. Biogeosciences. 2010;7(12):3961–9. doi:10.5194/bg-7-3961-2010.
  31. Tyagi M, da Fonseca MMR, de Carvalho CCCR. Bioaugmentation and biostimulation strategies in bioremediation. Biodegradation. 2011;22(2):231–41. doi:10.1007/s10532-010-9394-4.
  32. Wang Z, Fingas M, Page DS. Oil spill identification. J Chromatogr A. 1999;843(1–2):369–411. doi:10.1016/S0021-9673(99)00120-X.
Regular Issue Subscription Review Article
Volume 16
Issue 01
Received 06/03/2026
Accepted 19/03/2026
Published 10/04/2026
Publication Time 35 Days
18

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