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Hawraa Ghassan Hussein Salim,
Afrah Mahdi Aldhalimi,
Kawther Kadhem Abd-Alrudhe Hassan,
Abstract
The experiment was conducted during the 2022–2023 academic year in the laboratories of the Department of Biology, Faculty of Education for Girls, University of Kufa, to investigate the effects of varying concentrations of gibberellic acid (GA₃) — specifically 0, 2, and 3 ppm — and salicylic acid (SA) (0, 2.5, 5 ppm) as well as the interaction between them on the germination percentage and some growth traits of fenugreek seedlings and the production of the active compound Trigonelline. The results showed that treating fenugreek seeds with gibberellin at a concentration of 3 ppm and spraying the seedlings with salicylic acid at a concentration of 5 ppm gave the highest rate of germination percentage, number of leaves, shoot length, root length, fresh and dry weights of the seedling, reaching (86.82%, 5.54 leaves, 7.40 cm, 4.00 cm, 0.64 mg, 0.42 mg) respectively. Also, the results showed the highest accumulation of the alkaloid Trigonelline, reaching (0.99 µg) at the 5 ppm compared to the rest of the other treatments.
These findings suggest that the combined application of GA₃ and SA may enhance both early growth and secondary metabolite production in fenugreek. Such treatments could be beneficial for improving the medicinal value and agronomic performance of this important plant.
Keywords: Fenugreek, Trigonella foenum, gibberellic acid, Trigonelline, Germination
[This article belongs to Research and Reviews : A Journal of Biotechnology ]
Hawraa Ghassan Hussein Salim, Afrah Mahdi Aldhalimi, Kawther Kadhem Abd-Alrudhe Hassan. Study of the response of Fenugreek Seedlings (Trigonella foenum-graecum L.) to some Plant Growth Regulators and Their effect on the production of the active Compound (Trigonelline). Research and Reviews : A Journal of Biotechnology. 2025; 15(02):-.
Hawraa Ghassan Hussein Salim, Afrah Mahdi Aldhalimi, Kawther Kadhem Abd-Alrudhe Hassan. Study of the response of Fenugreek Seedlings (Trigonella foenum-graecum L.) to some Plant Growth Regulators and Their effect on the production of the active Compound (Trigonelline). Research and Reviews : A Journal of Biotechnology. 2025; 15(02):-. Available from: https://journals.stmjournals.com/rrjobt/article=2025/view=210064
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Research and Reviews : A Journal of Biotechnology
| Volume | 15 |
| Issue | 02 |
| Received | 30/04/2025 |
| Accepted | 08/05/2025 |
| Published | 13/05/2025 |
| Publication Time | 13 Days |
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