Investigations Into the Potential for Anthracene Decomposition of Novel Soil Bacterium, Pseudomonas putida P7

Year : 2024 | Volume : 14 | Issue : 02 | Page : 29-44
    By

    Kushalatha M.,

  • Umesh H.R.,

  1. Research Scholar, Departmen of Biochemistry, Maharani Lakshmi Ammanni College for Women, Bangalore, Karnataka, India
  2. Assistant Professor, Department of Biochemistry, Maharani Lakshmi Ammanni College for Women, Bengaluru, Karnataka, India

Abstract

Bioremediation of hazardous contaminants holds immense importance for the preservation of a clean and healthy environment. Conversely, the accumulation of toxicants can be mitigated with the assistance of microorganisms capable of breaking down these harmful substances into benign molecules. Anthracene is frequently used as a model molecule for studies on PAH pollution because it is found in many carcinogenic PAHs. Therefore, the hunt for new microorganisms with the ability to break down anthracene is necessary. This work intended to the soil of a petroleum refinery near Bangalore was used in this study to isolate twenty different bacterial strains. These strains were cultured on solid nutrient agar and then purified by cultivation on mineral media supplemented with anthracene. Successful bacterial growth and anthracene degradation were confirmed through optical density measurements. The growth of bacterial isolate was observed in media at a pH of 7.0 and temperature of 28°C. However, the presence of certain nitrogen and carbon sources inhibited bacterial growth during anthracene breakdown. The efficacy of anthracene degradation by the isolate )1.8×1022 colony forming units/ml and optical density of 0.92) was determined to be 85.36% after one hundred twenty hours of incubation. Gas chromatography-Mass spectrometer analysis revealed the conversion of anthracene to anthraquinone and 9, 10-dihydroxy-anthracene. Through the biochemical tests and 16S rRNA analysis, the highly effective bacterial isolate was identified as P7, a novel strain of Pseudomonas putida, with 98% homology. High-performance liquid chromatography analysis demonstrated the substantial reduction in anthracene levels (up to 83.25%) and the formation of reaction products by the bacterial strain P7 sourced from the soil. The newly identified bacterial strain P7 shows promise as an effective tool for bio-remediating hydrocarbon pollutants to protect the environment from polyaromatic hydrocarbon contaminants.

Keywords: Anthracene decomposition, strain, Pseudomonas, 16S rRNA, phylogenetic analysis

[This article belongs to Research and Reviews : A Journal of Biotechnology ]

How to cite this article:
Kushalatha M., Umesh H.R.. Investigations Into the Potential for Anthracene Decomposition of Novel Soil Bacterium, Pseudomonas putida P7. Research and Reviews : A Journal of Biotechnology. 2024; 14(02):29-44.
How to cite this URL:
Kushalatha M., Umesh H.R.. Investigations Into the Potential for Anthracene Decomposition of Novel Soil Bacterium, Pseudomonas putida P7. Research and Reviews : A Journal of Biotechnology. 2024; 14(02):29-44. Available from: https://journals.stmjournals.com/rrjobt/article=2024/view=166955



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Regular Issue Subscription Original Research
Volume 14
Issue 02
Received 28/03/2024
Accepted 08/04/2024
Published 14/08/2024
Publication Time 139 Days
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