Labeling transparency and heavy metal contamination in commercial protein powders: A case study

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This is an unedited manuscript accepted for publication and provided as an Article in Press for early access at the author’s request. The article will undergo copyediting, typesetting, and galley proof review before final publication. Please be aware that errors may be identified during production that could affect the content. All legal disclaimers of the journal apply.

Year : 2026 | Volume : 12 | 01 | Page :
    By

    Bavatharani V,

  • Amrita Shaw,

  • A. Sunil,

  • Jhinuk Gupta,

  1. Student, Department of Food and Nutritional Sciences, Sri Sathya Sai Institute of Higher Learning, Vidyagiri, Prasanthi Nilayam, Andhra Pradesh, India
  2. Allied PhD Research Scholar, Department of Food and Nutritional Sciences, Sri Sathya Sai Institute of Higher Learning, Vidyagiri, Prasanthi Nilayam, Andhra Pradesh, India
  3. Assistant Professor, Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Vidyagiri, Prasanthi Nilayam, Andhra Pradesh, India
  4. Assistant Professor,, Department of Food and Nutritional Sciences, Sri Sathya Sai Institute of Higher Learning, Vidyagiri, Prasanthi Nilayam, Andhra Pradesh, India

Abstract

With the recent surge in market demand, health supplements particularly protein powders, have become increasingly vulnerable to quality deterioration, adulteration, and unethical marketing practices. Non-compliance with labeling regulations is on a rise. Moreover, research indicates that heavy metals absorbed from contaminated soil often accumulate in the protein fractions of crops, thereby elevating the risk of heavy metal contamination in protein powders. These issues highlight the urgent need to systematically assess both mislabeling and heavy metal contamination in commercially available protein powders. In this study, 110 protein powders, available in India, were manually screened to check their compliance with Food Safety and Standards Authority of India (FSSAI) regulations and six of those, containing maximum number of violations, were assessed for heavy metal contamination through Microwave Plasma – Atomic Emission Spectroscopy (MP-AES). Target Hazard Quotients (THQs) and Hazard Index (HI) values were calculated to assess potential health risks of the heavy metal contamination. The findings revealed widespread mislabeling. Around 25% products lacked “how to use” instructions, increasing the risk of excessive protein intake and protein poisoning. 30% products omitted mandatory warning statements and 42% failed to mention potential allergens. 75% of cocoa-based powder contained stevia, a sweetener regulated by FSSAI in cocoa based formulations. Additionally, 8% of the samples had incomplete ingredient lists. The MP-AES analysis revealed Arsenic to be the most abundant heavy metal present in the studied samples, followed by lead and mercury. The calculated Estimated Daily Intake (EDI) and Hazard Indices (HI) of all six protein powders revealed the severity of the health risk associated with the regular consumption of protein powders over a period of time. These findings highlight the critical need for stringent quality control measures in the dietary supplement industry and the implementation of sustainable strategies to prevent heavy metal contamination in protein powdersa

Keywords: Protein powders; Heavy metal contamination; Mislabeling, MP-AES; Market survey.

How to cite this article:
Bavatharani V, Amrita Shaw, A. Sunil, Jhinuk Gupta. Labeling transparency and heavy metal contamination in commercial protein powders: A case study. International Journal of Nutritions. 2026; 12(01):-.
How to cite this URL:
Bavatharani V, Amrita Shaw, A. Sunil, Jhinuk Gupta. Labeling transparency and heavy metal contamination in commercial protein powders: A case study. International Journal of Nutritions. 2026; 12(01):-. Available from: https://journals.stmjournals.com/ijn/article=2026/view=242178


References

  1. Al-Garory NH, Al-Kaabi WJ. Determination of some heavy metals concentration in some dairy products from three different regions of Basrah, Iraq. Basrah Journal of Agricultural Sciences. 2020 Aug 12;33(2):1-3.
  2. Bethencourt-Barbuzano E, Gonzalez-Weller D, Paz-Montelongo S, Gutierrez-Fernandez AJ, Hardisson A, Carrascosa C, Camara M, Rubio-Armendariz C. Whey protein dietary supplements: metal exposure assessment and risk characterization. Nutrients. 2023 Aug 11;15(16):3543.
  3. Rodriguez-Lopez P, Rueda-Robles A, Sánchez-Rodríguez L, Blanca-Herrera RM, Quirantes-Piné RM, Borrás-Linares I, Segura-Carretero A, Lozano-Sánchez J. Analysis and screening of commercialized protein supplements for sports practice. Foods. 2022 Nov 3;11(21):3500.
  4. Thøgersen J. How does origin labelling on food packaging influence consumer product evaluation and choices? A systematic literature review. Food Policy. 2023 Aug 1;119:102503.
  5. Shangguan S, Afshin A, Shulkin M, Ma W, Marsden D, Smith J, Saheb-Kashaf M, Shi P, Micha R, Imamura F, Mozaffarian D. A meta-analysis of food labeling effects on consumer diet behaviors and industry practices. American journal of preventive medicine. 2019 Feb 1;56(2):300-14.
  6. Philips CA, Theruvath AH, Ravindran R, Chopra P. Citizens protein project: A self-funded, transparent, and concerning report on analysis of popular protein supplements sold in the Indian market. Medicine. 2024 Apr 5;103(14):e37724.
  7. Wu W, Wu P, Yang F, Sun DL, Zhang DX, Zhou YK. Assessment of heavy metal pollution and human health risks in urban soils around an electronics manufacturing facility. Science of the Total Environment. 2018 Jul 15;630:53-61.
  8. Meng R, Zhu Q, Long T, He X, Luo Z, Gu R, Wang W, Xiang P. The innovative and accurate detection of heavy metals in foods: A critical review on electrochemical sensors. Food Control. 2023 Aug 1;150:109743.
  9. Wong C, Roberts SM, Saab IN. Review of regulatory reference values and background levels for heavy metals in the human diet. Regulatory Toxicology and Pharmacology. 2022 Apr 1;130:105122.
  10. Li J, Zhang M, Sun J, Mao X, Wang J, Liu H, Zheng H, Li X, Zhao H, Zou D. Heavy metal stress-associated proteins in rice and Arabidopsis: genome-wide identification, phylogenetics, duplication, and expression profiles analysis. Frontiers in Genetics. 2020 May 8;11:477.
  11. Sarip SH, Aziz R. Sensory evaluation and quantitative analysis of mineral and heavy metal content of protein supplement incorporated with herbal extract. Malaysian Journal of Analytical Sciences. 2017;21(6):1299-306.
  12. Horváth IL, Kajner G, Galbács G, Csupor D. Analysis of heavy metal content in protein powders available on the Hungarian market: a reassuring snapshot, but not a reassuring quality guarantee. Journal of Nutritional Science. 2025 Jan;14: e49.
  13. Bandara SB, Towle KM, Monnot AD. A human health risk assessment of heavy metal ingestion among consumers of protein powder supplements. Toxicology reports. 2020 Jan 1; 7:1255-62.
  1. Regulation – 1881/2006 – EN – EUR-Lex [Internet]. Europa.eu. 2023 [cited 2026 Apr 30]. Available from: https://eur-lex.europa.eu/eli/reg/2006/1881/oj/eng
  2. ‌Ali H, Khan E. What are heavy metals? Long-standing controversy over the scientific use of the term ‘heavy metals’–proposal of a comprehensive definition. Toxicological & Environmental Chemistry. 2018 Jan 2;100(1):6-19.
  1. Kurzius-Spencer M, Burgess JL, Harris RB, Hartz V, Roberge J, Huang S, Hsu CH, O’Rourke MK. Contribution of diet to aggregate arsenic exposures—an analysis across populations. Journal of exposure science & environmental epidemiology. 2014 Mar;24(2):156-62.
  2. EFSA Panel on Contaminants in the Food Chain (CONTAM). Scientific Opinion on lead in food. EFSA journal. 2010 Apr;8(4):1570.
Ahead of Print Subscription Original Research
Volume 12
01
Received 16/04/2026
Accepted 30/04/2026
Published 30/04/2026
Publication Time 14 Days
26

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