A Sensitive SPE-ECD-GLC Procedure for Analyzing Simazine Quantitatively in Food Products

Year : 2025 | Volume : 03 | Issue : 01 | Page : 20 27
    By

    Anil Kumar,

  • Naisergik Deepika Khanna,

  1. Assistant Professor, Department of Chemistry, Government Degree College, Affiliated to Himachal Pradesh University, Nadaun, Shimla, Himachal Pradesh, India
  2. Assistant Professor, Department of Chemistry, Netaji Subhash Chander Bose Memorial Government College, Affiliated to Himachal Pradesh University, Hamirpur, Shimla, Himachal Pradesh, India

Abstract

Short and simple solid-phase extraction (SPE) method interfacing and hyphenating gas liquid chromatographic-electron capture detection (GLC-ECD) method has been developed for the qualification and quantification of simazine in some daily used agro products. United States Pharmacopeia G-42 gas chromatography stationary phase with ZB-35a, HTb Inferno-cZ (Zebron, Za) capillary column with an enlarged and extended temperature range of –60° to 400°C and electron capture detector was selected correlating some chemical, physical and structural features of Simazine. Attempted the higher temperature range which was programmed with 20–25 minutes having 0.94 ml/min of gas flow showing theoretical plates (~106) and optimum resolution (Rs ~ 2.04 min) with a limit of detection and limit of quantification values of 0.0013 μg/L and 0.13 μg/L, respectively. Accurate LOQ was achieved by using this method and is almost below the maximum residue concentrations recommended by the Indian Pharmacopeia Standards. Recoveries of the simazine obtained from agricultural samples by spiking ranged from 79.77 to 99.60% with relative standard deviations far below 3.17 %. More than five minutes of SPE residual workup makes the proposed method simple and fast having good economic importance for the quantitative analysis of simazine in common environmental institutes, medical colleges, and civil hospitals serving society.

Keywords: Simazine, LOD, LOQ, GLC-ECD, residues

[This article belongs to International Journal of Biochemistry and Biomolecule Research ]

How to cite this article:
Anil Kumar, Naisergik Deepika Khanna. A Sensitive SPE-ECD-GLC Procedure for Analyzing Simazine Quantitatively in Food Products. International Journal of Biochemistry and Biomolecule Research. 2025; 03(01):20-27.
How to cite this URL:
Anil Kumar, Naisergik Deepika Khanna. A Sensitive SPE-ECD-GLC Procedure for Analyzing Simazine Quantitatively in Food Products. International Journal of Biochemistry and Biomolecule Research. 2025; 03(01):20-27. Available from: https://journals.stmjournals.com/ijbbr/article=2025/view=196832


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Regular Issue Subscription Original Research
Volume 03
Issue 01
Received 23/11/2024
Accepted 16/12/2024
Published 03/02/2025
Publication Time 72 Days
156

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