Application Of Silver Nanoparticles On Experimental Wound Infection Of Pseudomonas aeruginosa In Rats

Year : 2025 | Volume : 02 | Issue : 01 | Page : 1 8
    By

    Wijdan Abdulghani Saleh Alhammadi,

  • Donia Suad Shakor,

  • Parzhin Sardar Mohammed,

  • Asmaa Jamal Mohammed,

  • Afaf Saud Hussein,

  • Mohannad Hamid Jasim,

  • Mariam Ahmed Kamal,

  1. Lecturer, Department of Biology, College of Science, University of Kirkuk, , Iraq
  2. Lecturer, Department of Biology, College of Science, University of Kirkuk, , Iraq
  3. Lecturer, Department of Biology, College of Science, University of Kirkuk, , Iraq
  4. Lecturer, Department of Biology, College of Science, University of Kirkuk, , Iraq
  5. Lecturer, Department of Biology, College of Science, University of Kirkuk, , Iraq
  6. Assistant Lecturer, Biotechnology and Environmental center, University of Fallujah, Iraq, , Iraq
  7. Assistant Lecturer, Department of Criminal Evidence College of Science, University of Kirkuk, , Iraq

Abstract

This study aimed to evaluate the application of silver nanoparticles on experimental wound infection of Pseudomonas aeruginosa in rats. Infected patients at a private hospital in Baghdad had fifty swab samples taken from their wounds and burns (25 samples in total). These swabs were cultured on primary medium and then routine bacteriological tests were done for identification. Molecular confirmation of bacteria was done by using 16S rRNA gene. The research found that out of 25 swabs taken from wound and burn regions, 15 (60%) and 16 (64%), respectively, of P. aeruginosa tested positive for the 16S rRNA gene. The bacterial suspension was applied to each wound bed after twenty-one rats were sedated and full-thickness skin wounds were made on their backs. Wound infection was evaluated by counting the total number of bacteria in the wound, and the healing process was seen under a microscope. The following treatments were given topically to the wound bed in all groups: G1 without treatment, G2 with bacterial infection but no therapy, and G3 with bacterial infection and subsequent treatment with 10 µL of AgNPs (0.04 mg/cm2). There is a tight relationship between the percentage of wound contraction in the AgNPs group and the other groups. The concentration of surface bacteria was shown to be lower in all groups treated with AgNPs by day 12 when compared to G2. The numbers of deep skin bacteria were significantly lower in the AgNPs group compared to the G2 group at any time point (P .

Keywords: P. aeruginosa, Nanoparticle, Wound infection, 16S rRNA, PCR.

[This article belongs to International Journal of Antibiotics ]

How to cite this article:
Wijdan Abdulghani Saleh Alhammadi, Donia Suad Shakor, Parzhin Sardar Mohammed, Asmaa Jamal Mohammed, Afaf Saud Hussein, Mohannad Hamid Jasim, Mariam Ahmed Kamal. Application Of Silver Nanoparticles On Experimental Wound Infection Of Pseudomonas aeruginosa In Rats. International Journal of Antibiotics. 2024; 02(01):1-8.
How to cite this URL:
Wijdan Abdulghani Saleh Alhammadi, Donia Suad Shakor, Parzhin Sardar Mohammed, Asmaa Jamal Mohammed, Afaf Saud Hussein, Mohannad Hamid Jasim, Mariam Ahmed Kamal. Application Of Silver Nanoparticles On Experimental Wound Infection Of Pseudomonas aeruginosa In Rats. International Journal of Antibiotics. 2024; 02(01):1-8. Available from: https://journals.stmjournals.com/ijab/article=2024/view=169733


References

  1. Rizzi SC, Upton Z, Bott K, Dargaville TR. Recent advances in dermal wound healing: Biomedical device approaches. Expert Rev Med Devices. 2010;7(1):143–54.
  2. Burke JP. Infection control—A problem for patient safety. N Engl J Med. 2003;348(7):651–6.
  3. Mahmoudi M, Gould LJ. Opportunities and challenges of the management of chronic wounds: a multidisciplinary viewpoint. Chronic Wound Care Manag Res. 2020;7:27–36.
  4. Reynolds D, Kollef M. The epidemiology, pathogenesis, and treatment of Pseudomonas aeruginosa infections: An update. Drugs. 2021;81(18):2117–31.
  5. Hameed FA. Isolation of Pseudomonas Aeruginosa and studying their resistance and Pyocyanin production. Kirkuk J Sci. 2024;19(1).
  6. Simonetti O, Rizzetto G, Radi G, Molinelli E, Cirioni O, Giacometti A, Offidani A. New perspectives on old and new therapies of staphylococcal skin infections: The role of biofilm targeting in wound healing. Antibiotics. 2021;10(11):1377.
  7. Ozhathil DK, Wolf SE. Prevention and treatment of burn wound infections: The role of topical antimicrobials. Expert Rev Anti Infect Ther. 2022;20(6):881–96.
  8. Snelling CF, Roberts FJ, Germann E, Gillespie K, Coderre S, Henry D. Comparison of standard and chlorhexidine-derivative topical antibacterial agents on the infected burned rat wound. Burns. 1988;14(2):91–100.
  9. Ignacio C, Barcellos L, Ferreira MD, Moura SA, Soares IA, Oréfice RL. In vivo tests of a novel wound dressing based on biomaterials with tissue adhesion controlled through external stimuli. J Mater Sci Mater Med. 2011;22(5):1357–64.
  10. Al-Hadeithi ZS, Jasim SA, Salahdin OD. Relation between resistance of Klebsiella pneumoniae to certain antibiotics and ESBL/PBP genes. Biodiversitas. 2022;23(8).
  11. Lansdown AB. Silver in health care: antimicrobial effects and safety in use. Biofunctional Text Skin. 2006;33:17–34.
  12. Zhang G, Niu A, Peng S, Jiang M, Tu Y, Li M, Wu C. Formation of novel polymeric nanoparticles. Acc Chem Res. 2001;34(3):249–56.
  13. Wani IA, Ahmad T. Size and shape dependent antifungal activity of gold nanoparticles: A case study of Candida. Colloids Surf B Biointerfaces. 2013;101:162–70.
  14. Martinez-Gutierrez F, Olive PL, Banuelos A, Orrantia E, Nino N, Sanchez EM, Ruiz F, et al. Synthesis characterization and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles. Nanomed Nanotechnol Biol Med. 2010;6(5):681–8.
  15. Mohanty S, Mishra S, Jena P, Jacob B, Sarkar B, Sonawane A. An investigation on the antibacterial cytotoxic and antibiofilm efficacy of starch-stabilized silver nanoparticles. Nanomed Nanotechnol Biol Med. 2012;8(6):916–24.
  16. Jiang B, Larson JC, Drapala PW, Pérez‐Luna VH, Kang‐Mieler JJ, Brey EM. Investigation of lysine acrylate containing poly (N‐isopropylacrylamide) hydrogels as wound dressings in normal and infected wounds. J Biomed Mater Res B Appl Biomater. 2012;100(3):668–76.
  17. Tian J, Wong KK, Ho CM, Lok CN, Yu WY, Che CM, Chiu JF, et al. Topical delivery of silver nanoparticles promotes wound healing. Chem Med Chem. 2007;2(1):129–36.
  18. Hatem ZA, Jasim SA, Mahdi ZH. Phenotypic and genotypic characterization of antibiotic resistance in Staphylococcus aureus isolated from different sources. Jundishapur J Microbiol. 2021;14(4).
  19. Jasim SA, Farhan SM. Variation of 16SrRNA gene for some nosocomial bacteria isolated from Ramadi Teaching Hospital for Women and Children. Indian J Forensic Med Toxicol. 2020;14(4).
  20. Baumans V, Van Loo PL. How to improve housing conditions of laboratory animals: The possibilities of environmental refinement. Vet J. 2013;195(1):24–32.
  21. Ziv-Polat O, Topaz M, Brosh T, Margel S. Enhancement of incisional wound healing by thrombin conjugated iron oxide nanoparticles. Biomaterials. 2010;31(4):741–7.
  22. Ahmad N. In vitro and in vivo characterization methods for evaluation of modern wound dressings. Pharmaceutics. 2022;15(1):42.
  23. Yasir M, Dutta D, Willcox MD. Mode of action of the antimicrobial peptide Mel4 is independent of Staphylococcus aureus cell membrane permeability. PLoS One. 2019;14(7).
  24. Weir E, Lawlor A, Whelan A, Regan F. The use of nanoparticles in anti-microbial materials and their characterization. Analyst. 2008;133(7):835–45.
  25. Singer M, Nambiar S, Valappil T, Higgins K, Gitterman S. Historical and regulatory perspectives on the treatment effect of antibacterial drugs for community-acquired pneumonia. Clin Infect Dis. 2008;47(Suppl 3).
  26. Kwan KH, Liu X, To MK, Yeung KW, Ho CM, Wong KK. Modulation of collagen alignment by silver nanoparticles results in better mechanical properties in wound healing. Nanomed Nanotechnol Biol Med. 2011;7(4):497–504.
  27. Williams RL, Sroussi HY, Abercrombie JJ, Leung K, Marucha PT. Synthetic decapeptide reduces bacterial load and accelerates healing in the wounds of restraint-stressed mice. Brain Behav Immun. 2012;26(4):588–96.
  28. Singer M, Nambiar S, Valappil T, Higgins K, Gitterman S. Historical and regulatory perspectives on the treatment effect of antibacterial drugs for community-acquired pneumonia. Clin Infect Dis. 2008;47(Suppl 3).
  29. Li Q, Mahendra S, Lyon DY, Brunet L, Liga MV, Li D, Alvarez PJ. Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications. Water Res. 2008;42(18):4591–602.
  30. Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv. 2009;27(1):76–83.
  31. Etheridge ML, Campbell SA, Erdman AG, Haynes CL, Wolf SM, McCullough J. The big picture on nanomedicine: the state of investigational and approved nanomedicine products. Nanomed Nanotechnol Biol Med. 2013;9(1):1–4.
  32. Pal S, Tak YK, Song JM. Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Appl Environ Microbiol. 2007;73(6):1712–20.
  33. Huang Z, Zheng X, Yan D, Yin G, Liao X, Kang Y, Yao Y, Huang D, Hao B. Toxicological effect of ZnO nanoparticles based on bacteria. Langmuir. 2008;24(8):4140–4.
  34. Jain D, Banerjee R. Comparison of ciprofloxacin hydrochloride‐loaded protein lipid and chitosan nanoparticles for drug delivery. J Biomed Mater Res B Appl Biomater. 2008;86(1):105–12.
  35. Maya S, Indulekha S, Sukhithasri V, Smitha KT, Nair SV, Jayakumar R, Biswas R. Efficacy of tetracycline encapsulated O-carboxymethyl chitosan nanoparticles against intracellular infections of Staphylococcus aureus. Int J Biol Macromol. 2012;51(4):392–9.
  36. Adams SB, Sabesan VJ, Easley ME. Wound healing agents. Foot Ankle Clin. 2006;11(4):745–51.
  37. Mahalakshmi P, Reshma G, Arthi C, Másson M, Rangasamy J. Biodegradable polymeric scaffolds and hydrogels in the treatment of chronic and infectious wound healing. Eur Polym J. 2023;180:112390.
  38. Gunasekaran T, Nigusse T, Dhanaraju MD. Silver nanoparticles as real topical bullets for wound healing. J Am Coll Clin Wound Spec. 2011;3(4):82–96.
  39. Robson MC, Mannari RJ, Smith PD, Payne WG. Maintenance of wound bacterial balance. Am J Surg. 1999;178(5):399–402.
Regular Issue Subscription Original Research
Volume 02
Issue 01
Received 06/08/2024
Accepted 16/08/2024
Published 30/08/2024
Publication Time 24 Days
331

Login


My IP

PlumX Metrics