Akshat Mishra,
Sandeep Gavel,
Cheshtarani Yadav,
DSVGK Kaladhar,
- Student, Department of Microbiology and Bioinformatics, Chhattisgarh, India
- Student, Department of Biotechnology, Guru Ghasidas University, Bilaspur,, Chhattisgarh, India
- Student, Department of Biotechnology, Guru Ghasidas University, Bilaspur, Chhattisgarh, India
- professor, Department of Microbiology and Bioinformatics, UTD, Atal Bihari Vajpayee University, Bilaspur, Chhattisgarh, India
Abstract
The rising demand for renewable and green energy sources has encouraged researchers to seek sustainable alternatives to fossil fuels. In the present study, an investigation was conducted on the synergistic production of biofuel from plant materials of Clitoria ternatea and Cymbopogon citratus with microbial fermentation by Saccharomyces cerevisiae. The study also evaluated the refractometric monitoring and trypsin digestion activity for the understanding of biochemical changes during biofuel production. The oil was obtained from the seeds of Clitoria ternatea and the dried leaves of Cymbopogon citratus using cold pressing machine. The extracted oil was further processed through transesterification with methanol, petroleum ether, and sodium hydroxide to prepare biofuel. The prepared biofuel mixture was added with yeast for fermentation studies. Changes in soluble compounds and fermentation activity were monitored by refractometric analysis for three consecutive days. The results showed that the samples with combined plant extracts and yeast have higher Brix values, indicating increased biochemical activity and better fermentation potential. The highest refractometric readings were obtained in the mixture of biofuel with both plant extracts and yeast from all samples, indicating efficient utilization of the substrate and synergistic interaction between plant biomass and microbial fermentation. In the trypsin digestion studies with the X-ray film assay, partial inhibition of proteolytic activity was observed in the plant extracts, which suggested the presence of bioactive compounds with probable trypsin inhibitory properties. Results of experiments indicate that synergistic interactions of Clitoria ternatea, Cymbopogon citratus and Saccharomyces cerevisiae can improve the efficiency of biofuel production. The work demonstrates the potential of plant biomass and microbial systems as sustainable resources for renewable energy production and provides a simple refractometric approach for monitoring biofuel synthesis.
Keywords: Biofuel production, Bioethanol, Saccharomyces cerevisiae, Microbial fermentation, Fermentation technology, Plant-based biofuel
[This article belongs to Research and Reviews : A Journal of Biotechnology ]
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Research and Reviews : A Journal of Biotechnology
| Volume | 16 | |
| Issue | 02 | |
| Received | 17/06/2026 | |
| Accepted | 14/07/2026 | |
| Published | 24/07/2026 | |
| Publication Time | 37 Days |