The Integrity of Bio-Stimulant Efficiency of Mint Leaf (Mentha Piperita) on Crude Oil Remediation in Soil Environment

Year : 2026 | Volume : 04 | Issue : 01 | Page : 40 46
    By

    Vector Chukwuemeka Ukpaka,

  • Joy Chukwuemeka Peter Ukpaka,

  • Abraham Peter Ukpaka,

  • Ukpaka Chukwuemeka. Peter,

  1. Research Student, Department of Industrial Engineering, Lyceum of the Philippines University, Cavite, Philippines
  2. Research Student, Department of Pharmacy, MSB Medical School Berlin GmbH – Hochschule für Gesundheit und Medizin, Berlin, Germany
  3. Research Student, Department of Computer Engineering, Lyceum of the Philippines University, Cavite, Philippines
  4. Professor, Department of Chemical/Petrochemical Engineering, Rivers State University Port Harcourt, Rivers State, Nigeria

Abstract

This research addresses the integrity of biostimulant efficiency of mint leaf (Mentha piperita) on crude oil remediation in soil environments. The soil environment consideration in this investigation is loamy and some soil properties were examined; obtained data revealed a nitrogen value of 5.82%, phosphorus value of 46.91mg/kg, potassium value of 417.51mg/kg, and hydrogen-utilizing bacteria (HUB) value of 13.19cfu/g for unpolluted loamy soil, whereas polluted soil with crude oil data are 1.02% nitrogen, 1.326mg/kg phosphorus, 42.18mg/kg potassium, and 81.6cfu/g HUB. The properties of the biostimulant used revealed a nitrogen concentration of 6.93%, phosphorus of 52.271mg/kg, potassium of 463.902mg/kg, and HUB of 10.21cfu/g for sun-dried mint leaf samples, whereas room-dried nitrogen value is 9.47%, phosphorus value is 74.113mg/kg, potassium value is 552.542mg/kg, and HUB value is 13.98cfu/g. The density and kinematic viscosity of the crude oil used are 0.861g/ml and 2.84mm²/s. Crude oil remediation was monitored for a period of 35 days and the percentage reduction in the total petroleum hydrocarbon concentration determined. Application of sun-dried biostimulant revealed TPH reduction values in terms of percentage removal within the range of 1.43% to 77.88% for intervals of 7 to 35 days with a 50g dosage of remediant and 4.51% to 91.14% for 100g dosage, whereas room-dried biostimulant application demonstrates 2.38% to 78.53% for 7 to 35 days of 50g dosage and 8.41% to 92.29% for 100g dosage of remediant. In the control sample, obtained data revealed 1.13% to 25.67% for the period of 7 to 35 days. This research revealed that mint leaf (Mentha piperita) characteristics possess the integrity of good biostimulant efficiency for use in treating soil contaminated with crude oil. However, the room-dried sample is higher in efficiency than sun-dried, comparing percentage reduction of TPH concentration from 2.38% to 78.53% for intervals of 7 to 35 days of 50g dosage against 1.43% to 77.83% for the sun-dried value. This investigation has demonstrated the significance of the biostimulant in crude oil remediation.

Keywords: Biostimulant, crude oil, efficiency, integrity, mint leaf, remediation, soil

[This article belongs to International Journal of Pollution: Prevention & Control ]

How to cite this article:
Vector Chukwuemeka Ukpaka, Joy Chukwuemeka Peter Ukpaka, Abraham Peter Ukpaka, Ukpaka Chukwuemeka. Peter. The Integrity of Bio-Stimulant Efficiency of Mint Leaf (Mentha Piperita) on Crude Oil Remediation in Soil Environment. International Journal of Pollution: Prevention & Control. 2026; 04(01):40-46.
How to cite this URL:
Vector Chukwuemeka Ukpaka, Joy Chukwuemeka Peter Ukpaka, Abraham Peter Ukpaka, Ukpaka Chukwuemeka. Peter. The Integrity of Bio-Stimulant Efficiency of Mint Leaf (Mentha Piperita) on Crude Oil Remediation in Soil Environment. International Journal of Pollution: Prevention & Control. 2026; 04(01):40-46. Available from: https://journals.stmjournals.com/ijppc/article=2026/view=241691


References

  1. Lovins AB. How big is the energy efficiency resource?. In: Smith J, editor. Energy Efficiency Systems. 1st edition. London, UK: Academic Press; 2018. pp. 1-15.
  2. Glaser RA, Shulman S, Klinger P. Data supporting a provisional American Society for Testing and Materials (ASTM) method for metalworking fluids. In: Brown T, editor. Industrial Fluid Testing. 2nd edition. City, Country: ASTM International; 1999. pp. 131–136.
  3. American Public Health Association. Standard methods for the examination of water and wastewater. In: Rice EW, editor. Standard Methods. 23rd edition. Washington, USA: APHA; 2017. pp. 100–500.
  4. Asim N, Badiei M, Torkashvand M. Wastes from the petroleum industries as sustainable resource materials in construction sectors. In: Sopian K, editor. Sustainable Construction Materials. 2nd edition. Selangor, Malaysia: UKM Press; 2021. pp. 1254–1259.
  5. Azeez ZA, AL-Jobori KM, Ali AJ. Isolation of Degraded Bacteria and Fungi of Petroleum Hydrocarbons from Two Polluted Soil. In: Ali AJ, editor. Iraqi Biotechnology Handbook. 1st edition. Baghdad, Iraq: University of Baghdad; 2021. pp. 20-35.
  6. Cole M, Lindeque P, Fileman E. Microplastic ingestion by zooplankton. In: Galloway TS, editor. Marine Pollution and Ecosystems. 1st edition. Exeter, UK: University of Exeter; 2013. pp. 6646–6655.
  7. Cui J, Chen H, Sun M, Wen J. Comparison of bacterial community structure and function under petroleum hydrocarbon degradation. In: Wen J, editor. Bioprocess Engineering. 4th edition. Beijing, China: Science Press; 2020. pp. 303–313.
  8. Dindar E, Şağban FO, Başkaya HS. Variations of soil enzyme activities in petroleum-hydrocarbon contaminated soil. In: Başkaya HS, editor. International Biodeterioration. 3rd edition. Bursa, Turkey: Uludag University; 2015. pp. 268–275.
  9. Di Toro DM, McGrath JA, Stubblefield WA. Predicting the toxicity of neat and weathered crude oil. In: Toro DM, editor. Environmental Toxicology. 1st edition. Newark, USA: University of Delaware; 2007. pp. 24–36.
  10. Walls WD. Petroleum refining industry in China. In: Walls WD, editor. Energy Policy in Asia. 1st edition. Calgary, Canada: University of Calgary; 2010. pp. 2110–2115.
  11. Ere W, Chie-Amadi GO, Amagbo LG. Variations in Properties of Hydrocarbon Contaminated Soil under Bio-Wastes Treatments. In: Ere W, editor. Advanced Academic Research. 1st edition. Port Harcourt, Nigeria: IJAAR; 2020. pp. 10-25.
  12. McGill WB. Soil restoration following oil spills-a review. In: McGill WB, editor. Canadian Petroleum Technology. 2nd edition. Alberta, Canada: University of Alberta; 1977. pp. 16-32.
  13. Huang Y, Pan H, Wang Q. Enrichment of the soil microbial community in the bioremediation of a petroleum-contaminated soil. In: Huang Y, editor. Chemosphere Advances. 1st edition. Nanjing, China: Nanjing University; 2019. pp. 265–271.
  14. Swannell RP, Lee K, McDonagh M. Field evaluations of marine oil spill bioremediation. In: Lee K, editor. Marine Microbiology. 1st edition. Dartmouth, Canada: Fisheries and Oceans Canada; 1996. pp. 342–365.
  15. Wu M, Li W, Dick WA. Bioremediation of hydrocarbon degradation in a petroleum-contaminated soil. In: Wu M, editor. Soil Microbiology and Population. 1st edition. Columbus, USA: Ohio State University; 2017. pp. 124–130.
  16. Ukpaka CP, Lezorghia SB, Nwosu H. Crude oil degradation in loamy soil using Neem root extracts: An experimental study. In: Ukpaka CP, editor. Advances in Soil Remediation. 1st edition. Port Harcourt, Nigeria: Rivers State University; 2020. pp. 160–167.
Regular Issue Subscription Original Research
Volume 04
Issue 01
Received 20/01/2026
Accepted 21/01/2026
Published 27/02/2026
Publication Time 38 Days
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