Rathi C R,
Thara Pradeep,
Prem Jose Vazhacharickal,
Sajeshkumar N K,
- Assistant Professor, Department of Biotechnology, Mar Augusthinose College Ramapuram, Kottayam, Kerala, India
- Assistant Professor, Department of Biotechnology, Mar Augusthinose College Ramapuram, Kottayam, Kerala, India
- Student, Student, Department of Agricultural Economics, University of Agricultural Economics, Bangalore, India
- Assistant Professor, Department of Biotechnology, Mar Augusthinose College Ramapuram, Kottayam, Kerala, India
Abstract
Petroleum pollutants pose a serious and persistent threat to environmental ecosystems, particularly affecting soil and water quality. Effective and sustainable remediation strategies are crucial to mitigate their impact and safeguard environmental health. In this context, bioremediation—using microorganisms to degrade harmful contaminant —emerges as a promising and eco-friendly approach. The present study explores the biodegradation capabilities of the bacterium Priestia megaterium, a species previously recognized for its ability to break down hydrocarbons. The primary objective of this research is to isolate and characterize Priestia megaterium from soil samples obtained
from areas in close proximity to a petrol pump, a site typically subjected to high levels of petroleum contamination. The study aims to evaluate the bacterium’s efficiency in degrading petroleum-based pollutants and investigate its applicability in bioremediation efforts. The research methodology involves the isolation of P. megaterium from petroleum-contaminated soil, followed by a comprehensive analysis through both biochemical assays and molecular characterization techniques. Further, the
study assesses the hydrocarbon degradation potential of the bacterium under controlled conditions. This investigation aspires to contribute to the development of sustainable, low-cost, and environmentally friendly bioremediation technologies. By validating the efficacy of Priestia megaterium in degrading petroleum hydrocarbons, the findings are expected to support its application in field-scale bioremediation programs. Ultimately, the study aims to inform future strategies and enhance the efficiency of microbial-based clean-up of petroleum-contaminated sites.
Keywords: Biochemical methods, Biodegradation potential, Bioremediation, Environmental sustainability, Hydrocarbon degradation, Molecular methods, Petroleum contaminants, Priestia megaterium.
[This article belongs to Research and Reviews : A Journal of Biotechnology ]
Rathi C R, Thara Pradeep, Prem Jose Vazhacharickal, Sajeshkumar N K. Application of Priestia Megaterium in the Bioremediation of Petroleum Hydrocarbon Contaminated Environments. Research and Reviews : A Journal of Biotechnology. 2026; 16(01):-.
Rathi C R, Thara Pradeep, Prem Jose Vazhacharickal, Sajeshkumar N K. Application of Priestia Megaterium in the Bioremediation of Petroleum Hydrocarbon Contaminated Environments. Research and Reviews : A Journal of Biotechnology. 2026; 16(01):-. Available from: https://journals.stmjournals.com/rrjobt/article=2026/view=238730
References
- Leahy, J. G., & Colwell, R. R. (1990). Microbial degradation of hydrocarbons in the environment. Microbiological Reviews, 54(3), 305–315. https://doi.org/10.1128/mr.54.3.305-315.1990
- Yakimov, M. M., Timmis, K. N., & Golyshin, P. N. (2007). Obligate oil-degrading marine bacteria. Current Opinion in Biotechnology, 18(3), 257–266. https://doi.org/10.1016/j.copbio.2007.04.006
- Das, N., & Chandran, P. (2011). Microbial degradation of petroleum hydrocarbon contaminants: An overview. Biotechnology Research International, 2011, 941810. https://doi.org/10.4061/2011/941810
- Atlas, R. M., & Hazen, T. C. (2011). Oil biodegradation and bioremediation: A tale of the two worst spills in U.S. history. Environmental Science & Technology, 45(16), 6709–6715. https://doi.org/10.1021/es2013227
- Prince, R. C., & Butler, J. D. (2014). A protocol for assessing the effectiveness of oil spill dispersants in stimulating the biodegradation of oil. Environmental Science & Pollution Research, 21(16), 9506–9510. https://doi.org/10.1007/s11356-013-2053-6
- Varjani, S. J., & Upasani, V. N. (2017). A new look on factors affecting microbial degradation of petroleum hydrocarbon pollutants. International Biodeterioration & Biodegradation, 120, 71–83. https://doi.org/10.1016/j.ibiod.2017.02.006
- Nguyen, V. T., Lan Anh, N. T., et al. (2021). Characterization of arsenic-resistant endophytic Priestia megaterium R2.5.2 isolated from ferns in an arsenic-contaminated multi-metal mine in Vietnam. Journal of Experimental Biology and Agricultural Sciences, 9(3). https://jebas.org/ojs/index.php/jebas/article/view/1039
- Siddiqui, Z., Grohmann, E., & Malik, A. (2023). Degradation of alkane hydrocarbons by Priestia megaterium ZS16 and sediment consortia. Chemosphere, 320, 137886. https://doi.org/10.1016/j.chemosphere.2023.137886
- Liu, X., Ji, J., Zhang, X., et al. (2024). Microbial remediation of crude oil in saline conditions by oil-degrading bacterium Priestia megaterium FDU301. Applied Biochemistry and Biotechnology, 196, 2694–2712. https://doi.org/10.1007/s12010-022-04245-4
- Ghazali, F. M., Rahman, R. N. Z. R. A., Salleh, A. B., & Basri, M. (2004). Biodegradation of hydrocarbons in soil by microbial consortium. International Biodeterioration & Biodegradation, 54(1), 61–67. https://doi.org/10.1016/j.ibiod.2004.02.002
- Darsa, K. V., Thatheyus, A. J., & Ramya, D. (2014). Biodegradation of petroleum compound using the bacterium Bacillus subtilis. DOI: 10.17311/sciintl.2014.20.25
- Oyetibo, G. O., Chien, M. F., Ikeda-Ohtsubo, W., Suzuki, H., Obayori, O. S., Adebusoye, S. A., Ilori, M. O., Amund, O. O., & Endo, G. (2017). Biodegradation of crude oil and phenanthrene by heavy metal-resistant Bacillus subtilis isolated from a multi-polluted industrial wastewater creek. International Biodeterioration & Biodegradation, 120, 143–151. https://dx.doi.org/10.1016/j.ibiod.2017.02.021
- Abd El-Rahim, W., & Moawad, H. (2025). The role of bioremediation in achieving environmental sustainability. IntechOpen. DOI: 10.5772/intechopen.1009485
- Vary, P. S., Biedendieck, R., Fuerch, T., Meinhardt, F., Rohde, M., Deckwer, W. D., & Jahn, D. (2007). Bacillus megaterium–from simple soil bacterium to industrial protein production host. Applied Microbiology and Biotechnology, 76(5), 957–967. https://doi.org/10.1007/s00253-007-1089-3
- Mukherjee, A. K., & Bordoloi, N. K. (2011). Bioremediation and reclamation of soil contaminated with petroleum oil hydrocarbons by exogenously seeded bacterial consortium: A pilot-scale study. Environmental Science and Pollution Research, 18(3), 471–478. https://doi.org/10.1007/s11356-010 0391-2
- Bento, F. M., Camargo, F. A., Okeke, B. C., & Frankenberger, W. T. (2005). Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation, and bioaugmentation. Bioresource Technology, 96(9), 1049–1055. https://doi.org/10.1016/j.biortech.2004.09.008
- Chikere, C. B., Tekere, M., & Adeleke, R. (2019). Enhanced microbial hydrocarbon biodegradation as stimulated during field-scale landfarming of crude oil-impacted soil. Sustainable Chemistry and Pharmacy, 14, 100177. https://doi.org/10.1016/j.scp.2019.100177.

Research and Reviews : A Journal of Biotechnology
| Volume | 16 |
| Issue | 01 |
| Received | 14/05/2025 |
| Accepted | 27/01/2026 |
| Published | 20/03/2026 |
| Publication Time | 310 Days |
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