Rakhi Pandey,
Garima Mathur,
- Research Scholar, Centre of Excellence for Microbial and Plant Biotechnology, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Uttar Pradesh, India, Uttar Pradesh, India
- Associate Professor, Centre of Excellence for Microbial and Plant Biotechnology, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Uttar Pradesh, India, Uttar Pradesh, India
Abstract
Polyhydroxyalkanoates (PHAs) are microbial polymers that have attracted substantial interest in recent times due to their use as biodegradable substitutes for petroleum-derived plastics and also as precursors for nano-enabled biomaterials. The aim of the present work was to evaluate the effect of static and shaking culture conditions on growth kinetics, biomass formation, and PHA synthesis by Priestia flexa JP1. The bacterial strain was cultured in mineral salt media (MSM) at 37 ± 0.2°C under static and shaking culture conditions (120 rpm) for 24-hour intervals. PHA was isolated using a conventional solvent extraction procedure and was weighed. Functional groups of the isolated polymer were analyzed using FTIR spectroscopy. Results showed that agitation led to enhanced PHA synthesis (0.47 g/L) when compared with static cultures (0.42 g/L), while the reverse was observed with biomass formation where static cultures yielded more biomass (0.65 g/L) than shaking cultures (0.57 g/L). Growth kinetics revealed better exponential growth under shaking condition showing increased oxygen transfer efficiency and increased metabolism. FTIR spectroscopy confirmed the presence of characteristic functional groups of PHA which include ester carbonyl group (C=O), C–O–C stretching, and aliphatic C–H bonds under both static and agitation conditions.
Keywords: Priestia flexa JP1, PHA, growth kinetics, FTIR, static, agitation
[This article belongs to Research and Reviews : A Journal of Biotechnology ]
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Research and Reviews : A Journal of Biotechnology
| Volume | 16 | |
| Issue | 02 | |
| Received | 07/08/2026 | |
| Accepted | 25/08/2026 | |
| Published | 05/09/2026 | |
| Publication Time | 29 Days |