Ashish Kushwaha,
Ritik Srivastava,
Arun K. Maurya,
Lalit Bisht,
Arvind Negi,
Lalita Kumari,
Shipra Omar,
Abhishek Chauhan,
- Assistant Professor, Department of Pharmacology, JBIT College of Pharmacy, Dehradun,, Uttarakhand,, India
- Assistant Professor, Department of Pharmacology, JBIT College of Pharmacy, Dehradun, Uttarakhand, India
- Professor, Department of Pharmaceutical Chemistry, JBIT College of Pharmacy, Dehradun, Uttarakhand, India
- Professor, Department of Pharmaceutics, JBIT College of Pharmacy, Dehradun, Uttarakhand, India
- Professor, Department of Pharmacognosy, JBIT College of Pharmacy, Dehradun, Uttarakhand, India
- Assistant Professor, Department of Pharmaceutical Chemistry, JBIT College of Pharmacy, Dehradun, Uttarakhand, India
- Assistant Professor, Department of Pharmacy Practice, School of Pharmaceutical Sciences, Shri Guru Ram Rai University, Dehradun, Uttarakhand, India
- Assistant Professor, Department of Pharmacognosy, Subharti Institute of Pharmacy, Dehradun, Uttarakhand, India
Abstract
Ensuring the safety and quality of transfusion products is a cornerstone of transfusion medicine. The presence of leucocytes in blood components can lead to adverse reactions and increase the likelihood of transfusion-related complications, highlighting the importance of minimizing their content prior to transfusion. Elevated leucocyte levels have been detected in unfiltered fresh frozen plasma (FFP) units prepared from whole blood processed after a short holding time (SHT) – the interval between blood collection and component separation. Protein concentration, another crucial indicator of plasma quality, must also be maintained within acceptable limits. In this study, 100 non-filtered FFP units processed after SHT were compared with 100 units processed following an overnight, long holding time (LHT). Unit volumes, residual leucocyte counts (RLC), and protein concentrations (PC) were measured and statistically analysed. Results demonstrated that longer holding times were associated with significantly lower RLC and higher PC. Statistical process control (SPC) analysis revealed that the SHT process was borderline to incapable of maintaining consistent product quality, even after outlier adjustments. These findings indicate that shorter holding times may compromise plasma uniformity and quality. Therefore, revising the minimum holding time is advisable to achieve optimal leucocyte reduction and protein preservation in FFP.
Keywords: Fresh frozen plasma, holding time, residual leucocytes, plasma proteins, leukoreduction, transfusion safety
[This article belongs to International Journal of Antibiotics ]
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International Journal of Antibiotics
| Volume | 03 | |
| Issue | 02 | |
| Received | 08/04/2026 | |
| Accepted | 11/04/2026 | |
| Published | 21/04/2026 | |
| Publication Time | 13 Days |