Isolation, Purification, and Identification of Antibiotic-Resistant Bacterial Strain from Food Stuffs

Year : 2026 | Volume : 03 | Issue : 02 | Page : 37 45
By

Kaushal Chaudhary,

Shikha Yadav,

  1. Research Scholar, Department of Microbiology, Chaudhary Charan Singh University, Meerut, Uttar Pradesh, India
  2. Research Scholar, Department of Microbiology, Chaudhary Charan Singh University, Meerut, Uttar Pradesh, India

Abstract

The widespread presence of bacteria in foodstuffs poses significant trouble to health and food safety. Pathogens in consumable products increases the transmission of resistance genes to human, potentially compromising the efficacy of conventional antibiotics. This study aims to isolate, purify, and identify antibiotic-resistant bacterial strains from food items, meals, and vegetables. The investigation employed selective culture techniques, antibiotic susceptibility assays, and molecular identification methods to detect and characterize resistant strains. Total of 120 food samples were collected from different retail outlets, and standard microbiological techniques were used for isolation. Resistant strains were further characterized using biochemical tests and 16S rRNA gene sequencing for precise identification. The results revealed a considerable presence of antibiotic-resistant strains in multiple food categories, highlighting the potential risk antimicrobial resistance (AMR). This study emphasizes necessity for stringent hygiene practices, responsible in agriculture, continuous monitoring of antibiotic resistance in products. Overall, this research provides crucial insights into the appearance and characteristics of antibiotic-resistant bacteria in foodstuffs and contributes to the understanding of their potential impact on health.

Keywords: Isolation and purification, antibiotic susceptibility testing, 16S rRNA sequencing, food safety, multidrug resistance, dairy products, ready-to-eat meals, public health

[This article belongs to Recent Trends in Infectious Diseases ]

How to cite this article: Kaushal Chaudhary, Shikha Yadav. Isolation, Purification, and Identification of Antibiotic-Resistant Bacterial Strain from Food Stuffs. Recent Trends in Infectious Diseases. 2026; 03(02):37-45.
How to cite this URL: Kaushal Chaudhary, Shikha Yadav. Isolation, Purification, and Identification of Antibiotic-Resistant Bacterial Strain from Food Stuffs. Recent Trends in Infectious Diseases. 2026; 03(02):37-45. Available from: https://journals.stmjournals.com/rtid/article=2026/view=252302

References

  1. Anderson RM. The pandemic of antibiotic resistance. Nat Med. 1999;5(2):147–9.
  2. Knowles R, Chandler C, O’Neill S, Sharland M, Mays N. A systematic review of national interventions and policies to optimize antibiotic use in healthcare settings in England. J Antimicrob Chemother. 2024;79(6):1234–47.
  3. Elbehiry A, Marzouk E, Abalkhail A, Edrees HM, Ellethy AT, Almuzaini AM, et al. Microbial food safety and antimicrobial resistance in foods: A dual threat to public health. Microorganisms. 2025;13(7):1592.
  4. Fusaro C, Miranda-Madera V, Serrano-Silva N, Bernal JE, Ríos-Montes K, González-Jiménez FE, et al. Antibiotic-resistant bacteria isolated from street foods: A systematic review. Antibiotics. 2024;13(6):481.
  5. Havelaar AH, Graveland H, van de Kassteele J, Zomer TP, Veldman K, Bouwknegt M. A summary index for antimicrobial resistance in food animals in the Netherlands. BMC Vet Res. 2017;13(1):305.
  6. Rajaei M, Moosavy MH, Gharajalar SN, Khatibi SA. Antibiotic resistance in the pathogenic foodborne bacteria isolated from raw kebab and hamburger: Phenotypic and genotypic study. BMC Microbiol. 2021;21(1):272.
  7. Aguilar-Montes de Oca S, Talavera-Rojas M, Soriano-Vargas E, Barba-León J, Vazquez-Navarrete J. Determination of extended spectrum β-lactamases/AmpC β-lactamases and plasmid-mediated quinolone resistance in Escherichia coli isolates obtained from bovine carcasses in Mexico. Trop Anim Health Prod. 2015;47(5):975–81.
  8. Landers TF, Cohen B, Wittum TE, Larson EL. A review of antibiotic use in food animals: Perspective, policy, and potential. Public Health Rep. 2012;127(1):4–22.
  9. Antunes P, Machado J, Peixe L. Characterization of antimicrobial resistance and class 1 and 2 integrons in Salmonella enterica isolates from different sources in Portugal. J Antimicrob Chemother. 2006;58(2):297–304.
  10. Marshall BM, Levy SB. Food animals and antimicrobials: Impacts on human health. Clin Microbiol Rev. 2011;24(4):718–33.
  11. Neyestani Z, Khademi F, Teimourpour R, Amani M, Arzanlou M. Prevalence and mechanisms of ciprofloxacin resistance in Escherichia coli isolated from hospitalized patients, healthy carriers, and wastewaters in Iran. BMC Microbiol. 2023;23(1):191.
  12. Ghosh K, Harikrishnan R, Mukhopadhyay A, Ringø E. Fungi and actinobacteria: Alternative probiotics for sustainable aquaculture. Fishes. 2023;8(12):575.
  13. Fisk AE. An on-farm study to determine the effects of oral administration of meloxicam post farrowing on sow body temperature and preweaning piglet performance. J Swine Health Prod. 2025;33(6):234–41.
  14. Chudasama M, Kumar SD, Bharath N, Chanu NS, Pradhan RC, Thivya P. Tackling multidrug-resistant Salmonella: Innovations for food safety and sustainability. BiotechIntellect. 2025;2(1):e4–1.
  15. Smith JL, Fratamico PM. Emerging and re-emerging foodborne pathogens. Foodborne Pathog Dis. 2018;15(12):737–57.
  16. França A, Gaio V, Lopes N, Melo LD. Virulence factors in coagulase-negative staphylococci. Pathogens. 2021;10(2):170.
  17. Somda NS, Tankoano A, Métuor-Dabiré A, Kaboré D, Bonkoungou JO, Kpoda DS, et al. A systematic review and meta-analysis of antibiotic resistance of foodborne pathogenic bacteria in West Africa between 2010 and 2020. J Food Prot. 2023;86(3):100061.
  18. Van Boeckel TP, Brower C, Gilbert M, Grenfell BT, Levin SA, Robinson TP, et al. Global trends in antimicrobial use in food animals. Proc Natl Acad Sci U S A. 2015;112(18):5649–54.
  19. Ventola CL. The antibiotic resistance crisis: Part 1: Causes and threats. P T. 2015;40(4):277.
  20. Verraes C, Van Boxstael S, Van Meervenne E, Van Coillie E, Butaye P, Catry B, et al. Antimicrobial resistance in the food chain: A review. Int J Environ Res Public Health. 2013;10(7):2643–69.
  21. Cohen ML. Antimicrobial resistance: Prognosis for public health. Trends Microbiol. 1994;2(10):422–5.
  22. Ajulo S, Awosile B. Global antimicrobial resistance and use surveillance system (GLASS 2022): Investigating the relationship between antimicrobial resistance and antimicrobial consumption data across the participating countries. PLoS One. 2024;19(2):e0297921.
  23. Alhassan MY, Kabara MK, Ahmad AA, Abdulsalam J, Habib HI. Revisiting antibiotic stewardship: Veterinary contributions to combating antimicrobial resistance globally. Bull Natl Res Cent. 2025;49(1):1–9.
  24. Chinemerem Nwobodo D, Ugwu MC, Oliseloke Anie C, Al-Ouqaili MT, Chinedu Ikem J, Victor Chigozie U, et al. Antibiotic resistance: The challenges and some emerging strategies for tackling a global menace. J Clin Lab Anal. 2022;36(9):e24655.
  25. Cheong KL, Zhang Y, Li Z, Li T, Ou Y, Shen J, et al. Role of polysaccharides from marine seaweed as feed additives for methane mitigation in ruminants: A critical review. Polymers (Basel). 2023;15(15):3153.
Regular Issue Subscription Review Article
Volume 03
Issue 02
Received 27/05/2026
Accepted 29/05/2026
Published 15/07/2026
Publication Time 49 Days

Login

My IP

PlumX Metrics