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 Special Issue Topics

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Open Access
Special Issue
Topic

Mechanics of Molecules and Thermodynamics analysis of plasmid DNA

Guest Editor:

        • Abstract Submission Deadline : 30/11/2023

        Manuscript Submission Deadline : 25/12/2023

        [This article belongs to Special Issue Mechanics of Molecules and Thermodynamics analysis of plasmid DNA under section ijbbr, ijbbr in (ijbbr, ijbbr)]

        Special Issue Description

        Isothermal titration calorimetry (ITC), dynamic light scattering, surface tension measurements, and UV spectroscopy were used to examine the chemical mechanism and thermodynamics of the interactions between plasmid DNA and cationic surfactants. The cationic surfactants investigated include cetylpyridinium chloride, benzyldimethyldodecylammonium chloride, and cetyl dimethyl tetradecyl ammonium chloride. The findings point to a critical aggregation concentration (CAC) of a surfactant, below which there is no discernible interaction between DNA and surfactant. Above the CAC, the surfactant clumps with plasmid DNA. Longer hydrocarbon chains in surfactants result in smaller CAC, suggesting that hydrophobic interaction is important for DNA-surfactant complexation. Additionally, an increase in ionic strength (I) raises the critical micellization concentration (CMC) while lowering the CAC (CMC). Due to these opposing effects, a critical ionic strength (I(c)) is reached at CAC = CMC; if I(c) is lower, CAC decreases; if I(c) is higher, DNA does not form complexes with surfactant micelles. Since the CAC is constant throughout a large range of DNA concentrations, DNA in the interaction displays a pseudo-phase feature. The ITC data demonstrated that the reaction is exclusively driven by entropy because both delta H(o) and deltaS(o) have positive values, ranging from roughly 70 to 110 J K(-1) mol(-1). The combination has a DNA phosphate to surfactant molar ratio of 0.63 to 0.05. Submicrometer-sized primary particles are produced by the process, which agglomerates at high surfactant concentrations.

        Editor Keywords

        Isothermal titration calorimetry , DNA , Critical micellization concentration , DNA phosphate , Plasmid DNA

        Manuscript Submission information

        Manuscripts should be submitted online by registering and logging in to this link. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed.
        Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the email address:[email protected] for announcement on this website.
        Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for the submission of manuscripts is available on the Instructions for Authors page.

        Participating journals:

        Journal Name

        International Journal of Biochemistry and Biomolecule Research, International Journal of Biochemistry and Biomolecule Research

        Abbrivation

        ijbbr, ijbbr

        ISSN

        Since

        2023

        APC

        950  $