HPLC and Tandem MS Device for the Recognition of Emtricitabine and Tenofovir Alafenamide in K2EDTA Human Plasma Utilizing Emtricitabine 15ND2 Tenofovir D5 as an IS

Open Access

Year : 2022 | Volume : 12 | Issue : 02 | Page : 30-38
By

    Arnav Walia

  1. Mansingh Rajput

  2. Neel Lahoti

  3. Vinayak Bhosale

  4. Arif Khan

  5. Rishikesh Yadav

  6. Snehal Patil

  1. Associate Research Trainee, Bioanalytical Research, Synergen Bio Pvt. Ltd.,, Maharashtra, India
  2. Head of Bio-analytical Research, h, Bioanalytical Research, Synergen Bio Pvt. Ltd., Maharashtra, India

Abstract

Developed very impressionable, discriminating, and exact extreme-efficiency liquid chromatographytandem bulk spectrometry form for measurement of Emtricitabine and Tenofovir Alafenamide in human plasma. Emtricitabine15ND2 Tenofovir D5 was used as a within the standard (IS). The samples were divided on Phenomenex Kinetex C18 5 μm 100A, 100*4.6 mm procession utilizing Acetonitrile: 0.2% formic acid, 60:40 V/V as movable state, the retention period of Emtricitabine: 1.36 minutes (±0.5 minutes), Emtricitabine 15ND2: 1.36 minutes (±0.5 minutes), Tenofovir Alafenamide: 1.41 minutes (±0.5 minutes) and Tenofovir Alafenamide D5: 1.41 minutes (±0.5 minutes) individually. The precursor to product ion evolutions of Emtricitabine 130.200/248.200 m/z and Tenofovir 176.200/477.250 m/z were used to discover and compute Emtricitabine and Tenofovir Alafenamide and Emtricitabine 15ND2 Tenofovir D5 individually. A Shimadzu LC-MS-8045 wholeequipped accompanying vehicle propelled by ejection of pressurized gas or liquid ion spray (TIS) source and conducted in multiple reactions monitoring (MRM) style was pre-owned for the study.

Keywords: Emtricitabine, LC-MS/MS, Chromatography, Extraction, Tenofovir, Antipsychotic, Depot Drug, HIV.

[This article belongs to Research & Reviews: A Journal of Toxicology(rrjot)]

How to cite this article: Arnav Walia, Mansingh Rajput, Neel Lahoti, Vinayak Bhosale, Arif Khan, Rishikesh Yadav, Snehal Patil HPLC and Tandem MS Device for the Recognition of Emtricitabine and Tenofovir Alafenamide in K2EDTA Human Plasma Utilizing Emtricitabine 15ND2 Tenofovir D5 as an IS rrjot 2022; 12:30-38
How to cite this URL: Arnav Walia, Mansingh Rajput, Neel Lahoti, Vinayak Bhosale, Arif Khan, Rishikesh Yadav, Snehal Patil HPLC and Tandem MS Device for the Recognition of Emtricitabine and Tenofovir Alafenamide in K2EDTA Human Plasma Utilizing Emtricitabine 15ND2 Tenofovir D5 as an IS rrjot 2022 {cited 2022 Aug 27};12:30-38. Available from: https://journals.stmjournals.com/rrjot/article=2022/view=97392

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References

1. Hernández-Tenorio R, Guzmán-Mar JL, Hinojosa-Reyes L et al. Determination of pharmaceuticals discharged in wastewater from a public hospital using lc-ms/ms technique. Journal of the Mexican Chemical Society. 2021; 65 (1): 94–108.
2. 2.Laxman, B., Yojana, K., Chaitali, K. A Rapid and Sensitive stability indicating Rp-HPLC method development for the quantitative analysis of empagliflozin & linagliptin in bulk & synthetic mixture. International Journal of Health Sciences.2022: 5526–5538. 3. Ocque AJ, Hagler CE, Morse GD, et al. Development and validation of an LC–MS/MS assay for tenofovir and tenofovir alafenamide in human plasma and cerebrospinal fluid. Journal of pharmaceutical and biomedical analysis. 2018; 156: 163–9. 4. Okahashi N, Yamada Y, Iida J, Matsuda F. Isotope Calculation Gadgets: A Series of Software for Isotope-Tracing Experiments in Garuda Platform. Metabolites. 2022; 12 (7): 646. 5. Patel SH, Ismaiel OA, Mylott Jr WR, et al. Simultaneous determination of intracellular concentrations of tenofovir, emtricitabine, and dolutegravir in human brain microvascular endothelial cells using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Analytica chimica acta. 2019; 1056: 79–87. 6. Šafranko S, Živković P, Stanković A, et al. Designing ColorX, image processing software for colorimetric determination of concentration, to facilitate students’ investigation of analytical chemistry concepts using digital imaging technology. Journal of Chemical Education. 2019; 96 (9): 1928–37. 7. Schauer AP, Sykes C, Cottrell ML, et al. Validation of an LC–MS/MS assay to simultaneously monitor the intracellular active metabolites of tenofovir, emtricitabine, and lamivudine in driedblood spots. Journal of pharmaceutical and biomedical analysis. 2018; 149: 40–5. 8. Shi H, Yu P. Correlation patterns prevalence, and co-occurrence of ergot alkaloids in cool-season adapted cereal grains revealed with molecular spectroscopy and LC-MS/MS equipped HPLC system. Food Chemistry. 2022: 133322. 9. Uszkoreit J, Barkovits K, Pacharra S, et al. Dataset containing physiological amounts of spike-in proteins into murine C2C12 background as a ground truth quantitative LC-MS/MS reference. Data in Brief. 2022; 43: 108435. 10. Vineetha VP, Asha G, Devika P. Withania somnifera attenuates Tilapia lake virus (TiLV)‐induced mortality by inhibiting stress and strengthening the innate antioxidant defence system. Aquaculture Research. 2021; 52 (11): 5493–505.


Regular Issue Open Access Article
Volume 12
Issue 02
Received August 8, 2022
Accepted August 20, 2022
Published August 27, 2022