Recent Advancements and Comprehensive Review on Hyphenated Techniques

Year : 2025 | Volume : 16 | Issue : 03 | Page : 37 50
    By

    Shreyas K. Reddy,

  • Anjali Nayak,

  • Devika H. G.,

  1. Assistant Professor, Department of Quality Assurance, Krupanidhi College of Pharmacy, Bangalore, Karnataka, India
  2. Assistant Professor, Department of Quality Assurance, Krupanidhi College of Pharmacy, Bangalore, Karnataka, India
  3. Assistant Professor, Department of Quality Assurance, Krupanidhi College of Pharmacy, Bangalore, Karnataka, India

Abstract

Hyphenated techniques represent a powerful class of analytical methods that combine two or more established techniques – typically a separation method with a spectroscopic detection technique – to achieve enhanced analytical performance. First introduced by Hirschfeld in 1980, the term “hyphenation” refers to the online coupling of such methods, enabling more precise, sensitive, and comprehensive analysis of complex samples. These techniques exploit the strengths of individual methods while overcoming their limitations, offering superior sensitivity, selectivity, and structural insight. Hyphenated techniques are indispensable in modern analytical chemistry due to their ability to analyse intricate biological, pharmaceutical, environmental, and material samples with greater speed and accuracy. Their applications span drug development, food safety, forensic analysis, and pollutant monitoring. Common examples include GC-MS, LC-MS, LC-IR, LC-NMR, CE-MS, and ICP-MS – each with distinct advantages and drawbacks. For instance, GC-MS offers excellent separation and detection of volatile compounds but may not handle thermally labile substances. LC-NMR provides structural data but is limited by sensitivity and cost. These integrated systems address critical challenges such as limited selectivity, low sensitivity, time consumption, and destructive analysis often encountered with standalone methods. They also help overcome technique incompatibility through innovations in interface design. Ongoing developments in hyphenated technologies are driven by the need for higher throughput, automation, and real-time monitoring. Recent advancements include miniaturized systems, enhanced detectors, and software algorithms for improved data processing. These innovations are broadening the scope of hyphenated techniques, cementing their role in advancing scientific discovery across disciplines.

Keywords: Hyphenation, analytical chemistry, separation techniques, spectroscopic detection, GC-MS, interface design

[This article belongs to Research and Reviews: A Journal of Pharmaceutical Science ]

How to cite this article:
Shreyas K. Reddy, Anjali Nayak, Devika H. G.. Recent Advancements and Comprehensive Review on Hyphenated Techniques. Research and Reviews: A Journal of Pharmaceutical Science. 2025; 16(03):37-50.
How to cite this URL:
Shreyas K. Reddy, Anjali Nayak, Devika H. G.. Recent Advancements and Comprehensive Review on Hyphenated Techniques. Research and Reviews: A Journal of Pharmaceutical Science. 2025; 16(03):37-50. Available from: https://journals.stmjournals.com/rrjops/article=2025/view=228680


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Regular Issue Subscription Review Article
Volume 16
Issue 03
Received 25/06/2025
Accepted 28/07/2025
Published 04/10/2025
Publication Time 101 Days
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