The Effect of Alcoholic, Aqueous and Crude Extracts of Lemon citrus Seeds on Staphylococcus and Klebsiella Bacteria

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This is an unedited manuscript accepted for publication and provided as an Article in Press for early access at the author’s request. The article will undergo copyediting, typesetting, and galley proof review before final publication. Please be aware that errors may be identified during production that could affect the content. All legal disclaimers of the journal apply.

Year : 2024 | Volume : 14 | Issue : 03 | Page : 32 36
    By

    Nada Mohammed Reda,

  • Zainab Assim Mahdi,

  • Ameen Abd Al-Ridha Hassan Shlash,

  • Jinan Mohammed Hussein,

  1. Assistant Professor, Department of Biology, College of Education for Girls, Kufa University, , Iraq
  2. Assistant Lecture, Department of Biology, College of Education for Girls, Kufa University, , Iraq
  3. Student, Department of Biology, College of Education for Girls, Kufa University, , Iraq
  4. Professor, Department of Biology, College of Education for Girls, Kufa University, , Iraq

Abstract

Five isolates of staphylococcus bacteria identified by phytic were obtained and activated on solid saline mannitol medium, where the colonies appeared in a golden color. Three extracts of dry lemon seeds were prepared (raw, 1% aqueous extract, 1% alcoholic extract). It was celebrated that the aqueous quotation was added operative on bacterial progress than the alcoholic and rudimentary sources. Three isolates of Klebsiella bacteria identified by VITEK were also obtained, and they were activated on solid MacConkey medium, and the colonies appeared in a pink color because they were fermented to the sugar lactose. The effect of the three extracts of dry lemon seeds (raw, 1% aqueous extract, 1% alcoholic extract) was studied. It was distinguished that the liquid isolated was extra active on bacterial evolution than the ethanolic and basic isolated extract. The derivation of the lemon is unidentified, however lemons are supposed to have chief developed in northeast India. A genomic revision of the lemon specified it was a mixture among bitter orange (bitter carroty) and citron. The derivation of the expression lemon could be Central Eastern. The term allurements from the Ancient French limon, then Italian limone, from the Arabic laymūn or līmūn, and from the Persian līmūn, a generic term for citrus berry, which is a similar of Sanskrit., Lemons essential a least hotness of nearby 7 °C (45 °F), so they are not resistant year-curved in moderate climates, but converted tougher as they matured. Citrus require minimal pruning by trimming overcrowded branches, with the tallest branch cut back to embolden bushy advance. During summer, larceny spinal tips of the greatest vigorous progression promises more profuse canopy enlargement. As mature plants may produce unwanted, fast-growing shoots

Keywords: Mannitol, staphylococcus, Klebsiella, bacteria, MacConkey.

[This article belongs to Research & Reviews: A Journal of Microbiology & Virology ]

How to cite this article:
Nada Mohammed Reda, Zainab Assim Mahdi, Ameen Abd Al-Ridha Hassan Shlash, Jinan Mohammed Hussein. The Effect of Alcoholic, Aqueous and Crude Extracts of Lemon citrus Seeds on Staphylococcus and Klebsiella Bacteria. Research & Reviews: A Journal of Microbiology & Virology. 2024; 14(03):32-36.
How to cite this URL:
Nada Mohammed Reda, Zainab Assim Mahdi, Ameen Abd Al-Ridha Hassan Shlash, Jinan Mohammed Hussein. The Effect of Alcoholic, Aqueous and Crude Extracts of Lemon citrus Seeds on Staphylococcus and Klebsiella Bacteria. Research & Reviews: A Journal of Microbiology & Virology. 2024; 14(03):32-36. Available from: https://journals.stmjournals.com/rrjomv/article=2024/view=170453


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References

  1. Al-Ratab AB. Study of bacteria causing contamination of surgical operations in Misrata Central Hospital and evaluation of antibiotic sensitivity to them. (Arabic). 2020.
  2. Saeed I. Identifying the types of bacteria that cause wound infections in patients at Sabratha Hospital in western Libya. (Arabic). Int Res J Sci Technol. 2020;5:1795–1801.
  3. Iqbal A, Arina ZB. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J. Ethnopharmacol. 2001;74:113–123.
  4. Jayana BL, Prasai T, Singh A, Yami AK. Study of antimicrobial activity of lime juice against Vibrio cholera. Sci World. 2010;8(8):44–46.
  5. Sashi KJ, Ramya M, Janardhan K. Antimicrobial activity ofethnomedicinal plants of Nilgiri Biosphere reserve and WesternGhats. Asian J. Microbiol. Biotechnol. Environ. Sci. 2003;5:183– 185.
  6. Hind AA, Samira KH. Isolation and identification of Proteus mirabilis bacteria from different clinical sources and investigation of some of their virulence factors. Al-Harf J. 2023 August;18:148–160.
  7. Sujatha S. Complementary and alternative therapies in palliative care: Atransition from modern medicine to traditional medicine in India. J. Cancer Pain and Symptom Palliation. 2005;1:25–9.
  8. Mothana RA, Lindequist U. Antimicrobial activity of some medicinal plants of the island Soqotra. J. Ethnopharmacol. 2005;96(1–2):177–181.
  9. Pathan RK, Gali PR, Pathan P, Gowtham T, Pasupuleti S. In vitro antimicrobial activity of Citrus aurantifolia and its phytochemical screening. Asian Pacific J Tropical Disease. 2012;2:s238– s331.
  10. Najm R, Jinan MH. Detection of virulence genes of pathogenic Escherichia coli isolated from different sources. Al-Harf J. 2023 August;18:130–138.
  11. Ndukwe KC, Okeke IN, Lamikanra A, Adesina SK, Aboderin O. Antibacterial activity of aqueous extracts of selected chewing sticks. J Contemp Dent Pract. 2005;(6)3:086–094.
  12. Adedayo O, Anderson WA, Moo-Young, Snieckus V, Patil PA, Kolawole DO. Phytochemistry and antibacteria activity of Senna alata flower. J Pharmaceu Biol. 2001;39:408–412.
  13. Onyeagba RA, Ugbogu OC, Okeke CU, Iroakasi O. Studies on the antimicrobial effects of garlic (Allium sativum Linn), ginger (Zingiber officinale Roscoe) and Lime (Citrus aurantifolia Linn). Afri J Biotechnol. 2004;3(10):552–554.
  14. Trease and Evans. Pharmacognosy. 15 edition, Published by Elsevier. 2005. pp. 266–267.
  15. Harborne JB. Phytochemical Method. Champman and Hall. London, New York. 1973.
  16. Egroove M. Detection the antimicrobial activity of plant extract in well. J food Agrianlture. B. 1985:122–496
  17. Bahaa AL, AL-Rubai, Hind Hussein Obaid, Tahrrer Hadi Saleh. Collage of Science. University of Baghdad, Iraq. 2016.
Regular Issue Subscription Original Research
Volume 14
Issue 03
Received 13/08/2024
Accepted 23/08/2024
Published 05/09/2024
399

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