Molecular Characterization of Circulating Rota Viral Strain causing infantile diarrhoea in Tripura, North East India

Open Access

Year : 2021 | Volume : | Issue : 3 | Page : 6-12
By

    Ankurita Bhowmik

  1. Apurba Sarkar

  2. Saikat Majumder

  3. Ayan Modak

  4. Niladri Sekhar Das

  5. Sanjib Kumar Debbarma

  6. Harpreet Kaur

  7. Tapan Majumdar

  1. Research Assistant, Department of Microbiology, Agartala Government Medical College, Agartala, Tripura, India
  2. Research Assistant, Department of Microbiology, Agartala Government Medical College, Agartala, Tripura, India
  3. Non-medical Scientist, Department of Microbiology, Agartala Government Medical College, Agartala, Tripura, India
  4. SRF, Department of Microbiology, Agartala Government Medical College, Agartala, Tripura, India
  5. Medical Scientist, Department of Microbiology, Agartala Government Medical College, Agartala, Tripura, India
  6. Professor, Department of Microbiology, Agartala Government Medical College, Agartala, Tripura, India
  7. Scientist F, Division of Epidemiology and Communicable Diseases, ICMR/DHR, MoHFW, New Delhi, India
  8. Professor, Department of Microbiology, Agartala Government Medical College, Agartala, Tripura, India

Abstract

Introduction: North Eastern India is geographically distinct from rest of India and the trend of Rota viral diarrhoea among children below 5 years of age group is not well studied yet. The Government of India introduced the oral rotavirus vaccine (ROTAVAC) in 2016 as part of the Universal Immunization Programme in different parts of India. Objective: (1). To determine the proportion of circulating Rotavirus strains causing acute viral gastroenteritis (AGE) among children below 5 years of age group. (2). Molecular characterization of Rotavirus genotypes. (3). Impact of vaccination on pattern of circulation of different Rotavirus genotype. Methods: Stool samples were collected between August 2016 to May 2019 and subjected to VP6 antigen detection of Group A Rotavirus by ELISA followed by genotyping by Semi-nested Polymerase Chain Reaction. The statistical analysis was done using Graph pad Prism 9. The categorical variables were distributed as proportions and the p-value was calculated by using Pearson’s chi-square test and Fisher’s exact test. p-Value of <0.05 was considered statistically significant. Results: Among the diarrheal episodes of the study subjects 39.3% was positive for rotavirus. G3P(8) was the predominant genotype followed by G1P(8). These genotypes alone contributed 51.1% and 23.8% respectively among the positive cases. A relatively new genotype, G9P(4) emerged during 2018–2019 with a prevalence of 6.8% that was not noticed during 2016–2017. Conclusion: This study highlights the importance of monitoring the trend of circulating rotavirus strains, which may help in appropriate management and control of Rotaviral diarrhoea among paediatric population

Keywords: Acute gastroenteritis, Rotavirus, Circulating Genotype, Semi-nested PCR, ROTAVAC

[This article belongs to Research & Reviews: A Journal of Microbiology & Virology(rrjomv)]

How to cite this article: Ankurita Bhowmik, Apurba Sarkar, Saikat Majumder, Ayan Modak, Niladri Sekhar Das, Sanjib Kumar Debbarma, Harpreet Kaur, Tapan Majumdar Molecular Characterization of Circulating Rota Viral Strain causing infantile diarrhoea in Tripura, North East India rrjomv 2021; 11:6-12
How to cite this URL: Ankurita Bhowmik, Apurba Sarkar, Saikat Majumder, Ayan Modak, Niladri Sekhar Das, Sanjib Kumar Debbarma, Harpreet Kaur, Tapan Majumdar Molecular Characterization of Circulating Rota Viral Strain causing infantile diarrhoea in Tripura, North East India rrjomv 2021 {cited 2021 Nov 29};11:6-12. Available from: https://journals.stmjournals.com/rrjomv/article=2021/view=92442

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References

1. Giri, S., Nair, N.P., Mathew, A. et al. Rotavirus gastroenteritis in Indian children < 5 years hospitalized for diarrhoea, 2012 to 2016. BMC Public Health 19, 69 (2019). https://doi.org/10.1186/s12889-019-6406-0.
2. Jacob John, Rajiv Sarkar, Jayaprakash Muliyil, et al., Rotavirus gastroenteritis in India, 2011–2013: Revised estimates of disease burden and potential impact of vaccines, Vaccine, Volume 32, Supplement 1,2014,Pages A5-A9,ISSN 0264-410X,https://doi.org/10.1016/j.vaccine.2014.03.004.
3. Bhandari N, Rongsen-Chandola T, Bavdekar A, et al. Efficacy of a monovalent human-bovine (116E) rotavirus vaccine in Indian infants: a randomised, double-blind, placebo-controlled trial. Lancet. 2014;383(9935):2136-2143. doi:10.1016/S0140-6736(13)62630-6.
4. World Health Organization. Manual of rotavirus detection and characterization methods. Geneva: World Health Organization; 2009.https://apps.who.int/iris/bitstream/handle/10665/70122/ WHO_IVB_08.17_eng.pdf?sequence=1&isAllowed=y. Accessed on 15th March 2021.
5. Gouvea, V, R I Glass, P Woods et al. “Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens.” Journal of clinical microbiology vol. 28,2 (1990): 276-82. doi:10.1128/JCM.28.2.276-282.1990.
6. Grimwood, K., Lambert, S.B. & Milne, R.J. Rotavirus Infections and Vaccines. Pediatr-Drugs 12, 235–256 (2010). https://doi.org/10.2165/11537200-000000000-00000.
7. Van Doorn LJ, Kleter B, Hoefnagel E, et al. Detection and genotyping of human rotavirus VP4 and VP7 genes by reverse transcriptase PCR and reverse hybridization. J ClinMicrobiol. 2009;47(9):2704-2712. doi:10.1128/JCM.00378-09.
8. Saluja T, Dhingra MS, Sharma SD, et al. Association of rotavirus strains and severity of gastroenteritis in Indian children. Hum Vaccin Immunother. 2017;13(3):711-716. doi:10.1080/21645515.2016.1238994.
9. Kang G, Desai R, Arora R, et al. Diversity of circulating rotavirus strains in children hospitalized with diarrhea in India, 2005-2009. Vaccine. 2013;31(27):2879-2883. doi:10.1016/j.vaccine.2013.04.030.
10. Babji S, Arumugam R, Sarvanabhavan A, et al. Multi-center surveillance of rotavirus diarrhea in hospitalized children <5 years of age in India, 2009-2012. Vaccine. 2014;32Suppl 1:A10-A12. doi:10.1016/j.vaccine.2014.03.001.
11. Saluja T, Sharma SD, Gupta M, et al. A multicenter prospective hospital-based surveillance to estimate the burden of rotavirus gastroenteritis in children less than five years of age in India. Vaccine. 2014;32Suppl 1:A13-A19. doi:10.1016/j.vaccine.2014.03.030.
12. Boni-cisse, C., Meite, S., Mlan, A.B. et al. Genotypic characterization of rotavirus in children under 5 years circulating in Côte D’Ivoire from 2010 to 2013. Virol J 15, 78 (2018). https://doi.org/10.1186/s12985-018-0973-z.
13. Tate JE, Burton AH, Boschi-Pinto C, et al. 2008 estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis. Lancet Infect Dis. 2012;12(2):136-141.
14. Kahn, G., Fitzwater, S., Tate, J. et al. Epidemiology and prospects for prevention of rotavirus disease in India. Indian Pediatr 49, 467–474 (2012). https://doi.org/10.1007/s13312-012-0076-7.
15. Anca IA, Furtunescu FL, Pleşca D, Streinu-Cercel A, Rugină S, Holl K. Hospital-based surveillance to estimate the burden of rotavirus gastroenteritis in children below five years of age in Romania. Germs. 2014;4(2):30-40. Published 2014 Jun 2. doi:10.11599/germs.2014.1053.
16. Rahman, M., Matthijnssens J, Yang X et al. Evolutionary history and global spread of the emerging g12 human rotaviruses. Journal of virology, 81(5), 2382–2390. https://doi.org/10.1128/JVI.01622-06.


Regular Issue Open Access Article
Volume 11
Issue 3
Received August 19, 2021
Accepted October 27, 2021
Published November 29, 2021