J. Samuel Kamanda,
Eric Kwasi Elliason,
Stephen Monday,
Atul Khajuria,
- Teaching Assistant, Faculty of Allied Health Sciences, Desh Bhagat University, Mandi Gobindgarh, Punjab, India
- Teaching Assistant, Faculty of Allied Health Sciences, Desh Bhagat University, Mandi Gobindgarh, Punjab, India
- Teaching Assistant, Faculty of Allied Health Sciences, Desh Bhagat University, Mandi Gobindgarh, Punjab, India
- Director, Faculty of Allied Health Sciences, Desh Bhagat University, Mandi Gobindgarh, Punjab, India
Abstract
Dengue virus (DENV) infection continues to be a public health concern in Punjab, India, where women are at higher risk because of socioeconomic factors. This cross-sectional study sought to assess the DENV seroprevalence, their neutralizing antibody profiles, and the risk factors associated with DENV among a sample of 1,200 women (aged 18–50 years) in Punjab. Serum samples were processed by IgG/IgM ELISA, and plaque reduction neutralization tests (PRNT90) were done to measure neutralizing antibodies. Findings showed that overall IgG seroprevalence was 48.0%, with higher rates among older age groups (57.8% in 41–50 year old women) and urban dwellers (52.4% vs. rural 41.7%). DENV₂ was the predominant serotype (37.2% multitypic immunity), but only 24.8% displayed monotypic immunity which suggests that most people were exposed to heterogeneous virus types. Urban residence (aOR=1.8), prolonged water storage (aOR=2.4), low income (aOR=1.5), and lack of mosquito nets (aOR=1.9) were other risk factors. This study highlights the need for effective vector control and vaccination programs and socioeconomic policies to reduce dengue burden among women in endemic areas.
Keywords: Aedes aegypti; dengue virus; neutralizing antibodies; Punjab; risk factors; seroprevalence; Women’s health
[This article belongs to Research and Reviews : A Journal of Immunology ]
J. Samuel Kamanda, Eric Kwasi Elliason, Stephen Monday, Atul Khajuria. Seroprevalence and Immunity to Dengue Virus Among Women in Punjab: A Public Health Concern. Research and Reviews : A Journal of Immunology. 2026; 16(01):6-10.
J. Samuel Kamanda, Eric Kwasi Elliason, Stephen Monday, Atul Khajuria. Seroprevalence and Immunity to Dengue Virus Among Women in Punjab: A Public Health Concern. Research and Reviews : A Journal of Immunology. 2026; 16(01):6-10. Available from: https://journals.stmjournals.com/rrjoi/article=2026/view=239743
References
- Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, et al. The global distribution and burden of dengue. Nature. 2013;496(7446):504–507.
- Sharma S, Kaur J, Singh H. Dengue outbreak in Punjab, 2022: Epidemiological and entomological insights. Indian J Public Health. 2023;67(1):45–50.
- Gupta N, Srivastava S, Jain A, Chaturvedi UC. Socioeconomic determinants of dengue transmission in rural India: A qualitative study. PLoS Negl Trop Dis. 2021;15(3):e0009255.
- Agarwal R, Kapoor S, Nagar R, Misra A. Seroprevalence of dengue in urban and rural populations in North India. Indian J Med Res. 2019;149(4):546–553.
- Murhekar MV, Kamaraj P, Kumar MS, Khan SA, Allam RR, Barde P. Burden of dengue infection in India, 2017: A cross-sectional population-based serosurvey. Lancet Glob Health. 2020;8(2):e224–e231.
- Wilder-Smith A, Vannice K, Durbin A, Hombach J, Thomas SJ, Thevarjan I, et al. Zika vaccines and therapeutics: Landscape analysis and challenges ahead. BMC Med. 2019;17(1):1–12.
- Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, et al. The global distribution and burden of dengue. Nature. 2013;496(7446):504–507.
- Gubler DJ. Dengue, urbanization and globalization: The unholy trinity of the 21st century. Trop Med Health. 2011;39(4 Suppl):3–11.
- Gupta BP, Sharma V, Lakshmi PVM. Socioeconomic determinants of dengue in Punjab: A case-control study. J Vector Borne Dis. 2021;58(2):123–129.
- Mehendale S. Dengue infection in India: A systematic review and meta-analysis. PLoS Negl Trop Dis. 2020;14(8):e0008333.
- Agarwal R, Singh N, Gupta P. Dengue seroprevalence in urban North India: A population-based survey. Indian J Med Res. 2019;150(3):249–254.
- Sharma S, Singh J, Kaur P. Dengue outbreak trends in Punjab, India: A five-year epidemiological analysis. J Infect Public Health. 2023;16(2):210–215.

Research and Reviews : A Journal of Immunology
| Volume | 16 |
| Issue | 01 |
| Received | 01/01/2026 |
| Accepted | 03/02/2026 |
| Published | 06/04/2026 |
| Publication Time | 95 Days |
Login
PlumX Metrics