To Investigate Hormonal Fluctuations in Male Rattus norvegicus after Treatment with 50% Methanolic Extract of Acacia arabica

Year : 2024 | Volume : 13 | Issue : 03 | Page : 28-38
    By

    Alka Qureshi,

  • Sonalika Singh,

  • Madhvi Senwelka,

  1. Scholar, Department of Zoology, Institute of Allied Medical Sciences and Technology. Nims University, Jaipur, Rajasthan, India
  2. Associate Professor, Department of Zoology, Institute of Allied Medical Sciences and Technology. Nims University, Jaipur, Rajasthan, India
  3. Assistant Professor, Department of Pathology, National Institute of Medical sciences. Nims University , Jaipur, Rajasthan, India

Abstract

The use of medicinal plants in traditional and modern medicine has garnered significant interest for their therapeutic potential. Acacia arabica, commonly known as babul, is renowned for its bioactive compounds and diverse pharmacological properties. This study investigates the hormonal fluctuations induced by 50% methanolic extract of Acacia arabica in male Rattus norvegicus, aiming to explore its potential as a male contraceptive. Male contraception remains limited primarily to condoms and vasectomy, necessitating the development of new, effective, and reversible methods. Because of their organic nature and reputed safety, organic contraception provides a possible option. Acacia arabica has shown antifertility effects in previous studies, suggesting it may interfere with spermatogenesis or hormonal regulation. the present research, sixty consecutive days of oral administration of Acacia arabica extract at dosages of 100, 200, and 300 mg/kg were given to male albino Wistar rats. To determine how it affected male reproductive health, the levels of the hormones testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were measured. Results indicated a dose-dependent reduction in sperm motility and density, with significant declines observed at higher doses. Fertility tests revealed decreased reproductive success, highlighting compromised sperm function and viability. Histopathological analysis of testicular tissues further supported these findings, showing dose-dependent degeneration of seminiferous tubules and reduction in spermatogenic cells. Similar degenerative changes were observed in the epididymis, seminal vesicles, and prostate gland, suggesting broad systemic effects of the extract on male reproductive organs. These findings underscore the potential of Acacia arabica as a modulator of male reproductive hormones and fertility. Further research is warranted to elucidate its mechanisms of action and assess long-term effects, aiming towards the development of novel, plant-based contraceptives that are both effective and safe.

Keywords: Acacia arabica, male contraception, spermatogenesis, hormonal fluctuations, Rattus norvegicus.

[This article belongs to Research & Reviews : Journal of Herbal Science ]

aWQ6MTc2ODE2fGZpbGVuYW1lOjcyMDBjMmE2LWZpLXBuZy53ZWJwfHNpemU6dGh1bWJuYWls
How to cite this article:
Alka Qureshi, Sonalika Singh, Madhvi Senwelka. To Investigate Hormonal Fluctuations in Male Rattus norvegicus after Treatment with 50% Methanolic Extract of Acacia arabica. Research & Reviews : Journal of Herbal Science. 2024; 13(03):28-38.
How to cite this URL:
Alka Qureshi, Sonalika Singh, Madhvi Senwelka. To Investigate Hormonal Fluctuations in Male Rattus norvegicus after Treatment with 50% Methanolic Extract of Acacia arabica. Research & Reviews : Journal of Herbal Science. 2024; 13(03):28-38. Available from: https://journals.stmjournals.com/rrjohs/article=2024/view=172357


Browse Figures

References

  1. Farnsworth NR. Screening plants for new medicines. In: Wilson EO, editor. Biodiversity. National Academy Press; 1988. p. 83–97.
  2. Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001;109(Suppl1):69–75.
  3. Newman DJ, Cragg GM. Natural products as sources of new drugs from 1981 to 2014. J Nat Prod. 2016;79(3):629–61.4.
    Farooq S, et al. Acacia arabica: Traditional uses and pharmacological properties. J Complement Integr Med. 2017;14(2):20160077.
  4. Parekh J, Chanda S. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk J Biol. 2007;31(1):538.
  5. Khalid M, Bilal A. Acacia arabica: An overview of its traditional uses, phytochemistry, and pharmacology. J Ethnopharmacol. 2020;257:112903.
  6. Sharma A, Kaur IP, Vaidya S. Bioactivity-guided fractionation and antimicrobial evaluation of the crude methanolic extract of Acacia arabica bark. Pharm Biol. 2010;48(7):753–8.
  7. Das S, Vasudeva N, Sharma S. Phytochemical analysis and free radical scavenging activity of Acacia arabica L. Int J Pharm Sci Res. 2011;2(5):1122–8.
  8. Uddin G, Rauf A, Patel S. Acacia arabica: A potential herb of prophylactic and therapeutic significance. Beni-Suef Univ J Basic Appl Sci. 2018;7(1):1–6.
  9. Sharma A, Kaur IP, Vaidya S. Bioactivity-guided fractionation and antimicrobial evaluation of the crude methanolic extract of Acacia arabica bark. Pharm Biol. 2010;48(7):753–758. doi:10.3109/13880200903290094
  10. Das S, Vasudeva N, Sharma S. Phytochemical analysis and free radical scavenging activity of Acacia arabica L. Int J Pharm Sci Res. 2011;2(5):1122–1128. Retrieved from https://ijpsr.com/
  11. Uddin G, Rauf A, Patel S. Acacia arabica: a potential herb of prophylactic and therapeutic significance. Beni-Suef Univ J Basic Appl Sci. 2018;7(1):1–6. doi:10.1016/j.bjbas.2017.09.003
  12. Gaur RD, Tiwari AK, Rai PK. Antifertility effects of Acacia nilotica extract on male rats. J Ethnopharmacol. 2013;150(2):804–12.
  13. Singh S, Singh SK, Kumar D. Effect of ethanolic extract of Acacia arabica bark on the reproductive system of male rats. Int J Pharm Sci Res. 2014;5(9):3842–6.
  14. Raji Y, Akinsomisoye OS, Salman TM. Antispermatogenic activity of Morinda lucida extract in male rats. Asian J Androl. 2006;8(1):69–73.
  15. Jeyaseelan I, Kumar PP, Manoharan S. Antifertility effects of various plant extracts in male albino rats. Int J Androl. 2007;30(2):135–40.
  16. Ratnasooriya WD, Dharmasiri MG. Effects of Acacia nilotica extract on male reproductive system in rats. Indian J Exp Biol. 2000;38(9):927–30.
  17. Sharma DK, Mali PC. Inhibition of spermatogenesis with the treatment of 50% methanolic extract of Maytenusemarginata leaves in albino rats. Int J Pharm Sci Res. 2016;7(8):3379–87.
  18. Lasheen SS, Refaat SH, El-Nefiawy NE, Abd-Elgawad RA, Othman AI. Developmental characteristics of rat testicular tissue and the impact of chronic noise stress exposure in the prenatal and postnatal periods. Anat Physiol Curr Res. 2015;4:1–15.
  19. Gautam M, Bhattacharya I, Devi YS, Arya SP, Majumdar SS. Hormone responsiveness of cultured Sertoli cells obtained from adult rats after their rapid isolation under less harsh conditions. Andrology. 2016;4:509–19.
  20. Majumdar SS, Tsuruta J, Griswold MD, Bartke A. Isolation and culture of Sertoli cells from the testes of adult Siberian hamsters. Biol Reprod. 1995;52:658–66.
  21. Salva A, Klinefelter GR, Hardy MP. Purification of rat Leydig cells: Increased yields after unit gravity sedimentation of collagenase dispersed interstitial cells. J Androl. 2001;22(4):665–71.
Regular Issue Subscription Original Research
Volume 13
Issue 03
Received 28/08/2024
Accepted 10/09/2024
Published 17/09/2024
Total Visits: 290


My IP

PlumX Metrics