Influence of Agitation and Static Cultivation on Physicochemical Characteristics of Bacterial Cellulose Produced by Gluconacetobacter liquefaciens MTCC 3135

Year : 2024 | Volume : | : | Page : –
By

Garima Singh

Pammi Gauba

Garima Mathur

  1. Research Scholar Department of Biotechnology, Jaypee Institute of Information Technology Uttar Pradesh India
  2. Professor Department of Biotechnology, Jaypee Institute of Information Technology Uttar Pradesh India
  3. Assistant Professor Department of Biotechnology, Jaypee Institute of Information Technology Uttar Pradesh India

Abstract

Bacterial cellulose (BC) is a versatile natural biopolymeric material, secreted by certain acetic acid bacteria. BC has numerous benefits over plant cellulose due to distinctive features, e.g. higher polymerization degree and purity, superior crystalline structure, water retention, biocompatibility, and biodegradability, which make BC a valuable material in creating sustainable and innovative solutions across various industries. However, the high operating cost, expensive culture media components and low productivity often limits its widespread industrial usage. In our study, BC production by Gluconacetobacter liquefaciens MTCC 3135 was analyzed under agitation and static cultivation in Hestrin–Schramm (HS) medium. Variations were observed in microbial growth kinetics parameters of G. liquefaciens under agitation and static cultivation. Highest BC yield at 3.55 ± 0.26 g/L was obtained in static cultivation, while agitated culture condition yielded the lowest BC at 2.59 ± 0.16 g/L. BC samples produced under agitation and static culture were purified using NaOH treatment and were subjected to physicochemical characterization using FTIR, XRD and DSC. FTIR spectra showed peak shifting and variations in peak intensities for BC samples produced under agitation and static culture, while compared to commercial cellulose (Himedia, India). BC produced under static culture was more crystalline compared to BC samples produced under agitation, as determined by FTIR and XRD. The research contributes valuable insights into alternative sources of BC production, targeting to fill gaps in knowledge and promote sustainable cellulose production

Keywords: Bacterial cellulose, Gluconacetobacter liquefaciens, Static, Agitation, FTIR, XRD, DSC

How to cite this article: Garima Singh, Pammi Gauba, Garima Mathur. Influence of Agitation and Static Cultivation on Physicochemical Characteristics of Bacterial Cellulose Produced by Gluconacetobacter liquefaciens MTCC 3135. Research & Reviews : A Journal of Biotechnology. 2024; ():-.
How to cite this URL: Garima Singh, Pammi Gauba, Garima Mathur. Influence of Agitation and Static Cultivation on Physicochemical Characteristics of Bacterial Cellulose Produced by Gluconacetobacter liquefaciens MTCC 3135. Research & Reviews : A Journal of Biotechnology. 2024; ():-. Available from: https://journals.stmjournals.com/rrjobt/article=2024/view=145768





References

  1. Naomi, R., Bt Hj Idrus, R. and Fauzi, M.B., 2020. Plant-vs. Bacterial-derived cellulose for wound healing: A review. International journal of environmental research and public health, 17(18), p.6803.
  2. Liu, Y., Ahmed, S., Sameen, D.E., Wang, Y., Lu, R., Dai, J., Li, S. and Qin, W., 2021. A review of cellulose and its derivatives in biopolymer-based for food packaging application. Trends in Food Science & Technology, 112, pp.532-546.
  3. Singh, A.K., Itkor, P. and Lee, Y.S., 2023. State-of-the-Art Insights and potential applications of Cellulose-Based hydrogels in food packaging: Advances towards sustainable trends. Gels, 9(6), p.433.
  4. Mokhena, T.C. and John, M.J., 2020. Cellulose nanomaterials: New generation materials for solving global issues. Cellulose, 27, pp.1149-1194.
  5. Davis, C.A., 2022. Advanced overview of bacterial nanocellulose manufacturing for novel applications. Analysis of biosynthesis, chemical treatments, processing, and properties (Master’s thesis, Universitat Politècnica de Catalunya).
  6. Han, T., New, N. and Win, P., 2018. Bacterial cellulose and its applications. In Handbook of Biopolymers (pp. 183-222). Jenny Stanford Publishing.
  7. Raut, M.P., Asare, E., Syed Mohamed, S.M.D., Amadi, E.N. and Roy, I., 2023. Bacterial cellulose-based blends and composites: Versatile biomaterials for tissue engineering applications. International Journal of Molecular Sciences, 24(2), p.986.
  8. Sharma, P., Mathur, G., Dhakate, S.R., Chand, S., Goswami, N., Sharma, S.K. and Mathur, A., 2016. Evaluation of physicochemical and biological properties of chitosan/poly (vinyl alcohol) polymer blend membranes and their correlation for Vero cell growth. Carbohydrate polymers, 137, pp.576-583.
  9. Dey, B., Behera, B., Parvathy, K.K., Jayaraman, S. and Paramasivan, B., 2023. Functionalized Bacterial Cellulose-Based Biopolymers for Biomedical Applications: Current Research Trends and Challenges. Biotic Resources, pp.175-191.
  10. Sharma, G., Thakur, B., Naushad, M., Kumar, A., Stadler, F.J., Alfadul, S.M. and Mola, G.T., 2018. Applications of nanocomposite hydrogels for biomedical engineering and environmental protection. Environmental chemistry letters, 16, pp.113-146.
  11. Li, J., Wu, Y., Yao, X., Tian, Y., Sun, X., Liu, Z., Ye, X. and Wu, C., 2023. Preclinical Research of Stem Cells: Challenges and Progress. Stem Cell Reviews and Reports, 19(6), pp.1676-1690.
  12. Ul-Islam, M., Khan, S., Fatima, A., Ahmad, M.W., Khan, M.S., Islam, S.U., Manan, S. and Ullah, M.W., 2022. Production of bio-cellulose from renewable resources: Properties and applications. In Renewable Polymers and Polymer-Metal Oxide Composites (pp. 307-339). Elsevier.
  13. Katyal, M., Singh, R., Mahajan, R., Sharma, A., Gupta, R., Aggarwal, N.K. and Yadav, A., 2023. Bacterial cellulose: Nature’s greener tool for industries. Biotechnology and Applied Biochemistry, 70(5), pp.1629-1640.
  14. Suryanto, H., Yanuhar, U., Mansingh, B.B. and Binoj, J.S., 2022. Bacterial Nanocellulose From Agro-Industrial Wastes. In Handbook of Biopolymers (pp. 1-39). Singapore: Springer Nature Singapore.
  15. Moreno, A.D., Alvira, P., Ibarra, D. and Tomás-Pejó, E., 2017. Production of ethanol from lignocellulosic biomass. Production of platform chemicals from sustainable resources, pp.375-410.
  16. Pasaribu, K.M., Masruchin, N. and Karina, M., 2024. Potential Application of Agro-Industrial Byproduct for Bacterial Cellulose Production; Its Challenges and Emerging Trends for Food Packaging. Biomass Conversion and Sustainable Biorefinery: Towards Circular Bioeconomy, pp.43-66.
  17. Singh, R., Mathur, A., Goswami, N. and Mathur, G., 2016. Effect of carbon sources on physicochemical properties of bacterial cellulose produced from Gluconacetobacter xylinus MTCC 7795. e-Polymers, 16(4), pp.331-336.
  18. Hestrin, S. and Schramm, M.J.B.J., 1954. Synthesis of cellulose by Acetobacter xylinum. 2. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. Biochemical Journal, 58(2), p.345.
  19. Mohammadkazemi, F., Azin, M. and Ashori, A., 2015. Production of bacterial cellulose using different carbon sources and culture media. Carbohydrate polymers, 117, pp.518-523.
  20. Zeng, X., Small, D.P. and Wan, W., 2011. Statistical optimization of culture conditions for bacterial cellulose production by Acetobacter xylinum BPR 2001 from maple syrup. Carbohydrate Polymers, 85(3), pp.506-513.
  21. Wang, S.S., Han, Y.H., Chen, J.L., Zhang, D.C., Shi, X.X., Ye, Y.X., Chen, D.L. and Li, M., 2018. Insights into bacterial cellulose biosynthesis from different carbon sources and the associated biochemical transformation pathways in Komagataeibacter sp. Polymers, 10(9), p.963.
  22. Mikkelsen, D., Flanagan, B.M., Dykes, G.A. and Gidley, M.J., 2009. Influence of different carbon sources on bacterial cellulose production by Gluconacetobacter xylinus strain ATCC 53524. Journal of applied microbiology, 107(2), pp.576-583.
  23. Jayalakshmi, A., Sivarajasekar, N., Kumar, M. and Mekala, V., 2022, November. Growth kinetics of cellulose producing bacteria. In AIP Conference Proceedings (Vol. 2446, No. 1). AIP Publishing.
  24. Kovárová-Kovar, K. and Egli, T., 1998. Growth kinetics of suspended microbial cells: from single-substrate-controlled growth to mixed-substrate kinetics. Microbiology and molecular biology reviews, 62(3), pp.646-666.
  25. Srivastava, S. and Mathur, G., 2022. Komagataeibacter saccharivorans strain BC-G1: an alternative strain for production of bacterial cellulose. Biologia, 77(12), pp.3657-3668.
  26. Mohite, B.V. and Patil, S.V., 2014. Physical, structural, mechanical and thermal characterization of bacterial cellulose by hansenii NCIM 2529. Carbohydrate polymers, 106, pp.132-141.
  27. Rusdi, R.A.A., Halim, N.A., Nurazzi, M.N., Abidin, Z.H.Z., Abdullah, N., Che Ros, F., Ahmad, N. and Azmi, A.F.M., 2022. Pre-treatment effect on the structure of bacterial cellulose from Nata de Coco (Acetobacter xylinum). Polimery, 67(3).
  28. Nelson, M.L. and O’Connor, R.T., 1964. Relation of certain infrared bands to cellulose crystallinity and crystal lattice type. Part II. A new infrared ratio for estimation of crystallinity in celluloses I and II. Journal of Applied Polymer Science, 8(3), pp.1325-1341.
  29. Bandyopadhyay, S., Saha, N. and Sáha, P., 2018. Characterization of bacterial cellulose produced using media containing waste apple juice. Applied biochemistry and microbiology, 54(6), pp.649-657.
  30. Mohite, B.V., Koli, S.H., Rajput, J.D., Patil, V.S., Agarwal, T. and Patil, S.V., 2019. Production and characterization of multifacet exopolysaccharide from an agricultural isolate, Bacillus subtilis. Biotechnology and Applied Biochemistry, 66(6), pp.1010-1023.
  31. Segal, D., 1991. Chemical synthesis of advanced ceramic materials (No. 1). Cambridge University Press.
  32. Colombo, A., Ribotta, P.D. and LEOn, A.E., 2010. Differential scanning calorimetry (DSC) studies on the thermal properties of peanut proteins. Journal of agricultural and food chemistry, 58(7), pp.4434-4439.
  33. Vasconcelos, N.F., Andrade, F.K., Vieira, L.D.A.P., Vieira, R.S., Vaz, J.M., Chevallier, P., Mantovani, D., Borges, M.D.F. and Rosa, M.D.F., 2020. Oxidized bacterial cellulose membrane as support for enzyme immobilization: properties and morphological features. Cellulose, 27(6), pp.3055-3083.
  34. Singh, R., Mathur, A., Goswami, N. and Mathur, G., 2016. Effect of carbon sources on physicochemical properties of bacterial cellulose produced from Gluconacetobacter xylinus MTCC 7795. e-Polymers, 16(4), pp.331-336.
  35. Vigentini, I., Fabrizio, V., Dellacà, F. and Foschino, R., 2019. Set-up of bacterial cellulose production from the genus Komagataeibacter and its use in a gluten-free bakery product as a case study. Frontiers in microbiology, 10, p.475675.
  36. Lahiri, D., Nag, M., Dutta, B., Dey, A., Sarkar, T., Pati, S., Edinur, H.A., Abdul Kari, Z., Mohd Noor, N.H. and Ray, R.R., 2021. Bacterial cellulose: Production, characterization, and application as antimicrobial agent. International journal of molecular sciences, 22(23), p.12984.
  37. Lahiri, D., Nag, M., Dutta, B., Dey, A., Sarkar, T., Pati, S., Edinur, H.A., Abdul Kari, Z., Mohd Noor, N.H. and Ray, R.R., 2021. Bacterial cellulose: Production, characterization, and application as antimicrobial agent. International journal of molecular sciences, 22(23), p.12984.
  38. Chandana, A., Mallick, S.P., Dikshit, P.K., Singh, B.N. and Sahi, A.K., 2022. Recent developments in bacterial nanocellulose production and its biomedical applications. Journal of Polymers and the Environment, 30(10), pp.4040-4067.
  39. Wong, S.S., Kasapis, S. and Tan, Y.M., 2009. Bacterial and plant cellulose modification using ultrasound irradiation. Carbohydrate Polymers, 77(2), pp.280-287.
  40. Ciolacu, D., Ciolacu, F. and Popa, V.I., 2011. Amorphous cellulose—structure and characterization. Cellulose chemistry and technology, 45(1), p.13.
  41. Wang, S.S., Han, Y.H., Chen, J.L., Zhang, D.C., Shi, X.X., Ye, Y.X., Chen, D.L. and Li, M., 2018. Insights into bacterial cellulose biosynthesis from different carbon sources and the associated biochemical transformation pathways in Komagataeibacter sp. Polymers, 10(9), p.963.
  42. Czaja, W., Romanovicz, D. and Brown, R.M., 2004. Structural investigations of microbial cellulose produced in stationary and agitated culture. Cellulose, 11, pp.403-411.
  43. Mohite, B.V. and Patil, S.V., 2014. Physical, structural, mechanical and thermal characterization of bacterial cellulose by G. hansenii NCIM 2529. Carbohydrate polymers, 106, pp.132-141.
  44. Thongwai, N., Futui, W., Ladpala, N., Sirichai, B., Weechan, A., Kanklai, J. and Rungsirivanich, P., 2022. Characterization of bacterial cellulose produced by Komagataeibacter maltaceti P285 isolated from contaminated honey wine. Microorganisms, 10(3), p.528.
  45. Meng, F.H., Schricker, S.R., Brantley, W.A., Mendel, D.A., Rashid, R.G., Fields Jr, H.W., Vig, K.W. and Alapati, S.B., 2007. Differential scanning calorimetry (DSC) and temperature-modulated DSC study of three mouthguard materials. Dental Materials, 23(12), pp.1492-1499.
  46. Vasconcelos, N.F., Feitosa, J.P.A., da Gama, F.M.P., Morais, J.P.S., Andrade, F.K., de Souza, M.D.S.M. and de Freitas Rosa, M., 2017. Bacterial cellulose nanocrystals produced under different hydrolysis conditions: Properties and morphological features. Carbohydrate polymers, 155, pp.425-431.
  47. Potivara, K. and Phisalaphong, M., 2019. Development and characterization of bacterial cellulose reinforced with natural rubber. Materials, 12(14), p.2323.
  48. Oliveira, R.L., Vieira, J.G., Barud, H.S., Assunção, R., R Filho, G., Ribeiro, S.J. and Messadeqq, Y., 2015. Synthesis and characterization of methylcellulose produced from bacterial cellulose under heterogeneous condition. Journal of the Brazilian Chemical Society, 26, pp.1861-1870.

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Received March 28, 2024
Accepted April 8, 2024
Published May 10, 2024