Influence of Agitation and Static Cultivation on Physicochemical Characteristics of Bacterial Cellulose Produced by Gluconacetobacter liquefaciens MTCC 3135

Year : 2024 | Volume : | : | Page : –
By

Garima Singh

Pammi Gauba

Garima Mathur

  1. Research Scholar Department of Biotechnology, Jaypee Institute of Information Technology Uttar Pradesh India
  2. Professor Department of Biotechnology, Jaypee Institute of Information Technology Uttar Pradesh India
  3. Assistant Professor Department of Biotechnology, Jaypee Institute of Information Technology Uttar Pradesh India

Abstract

Bacterial cellulose (BC) is a versatile natural biopolymeric material, secreted by certain acetic acid bacteria. BC has numerous benefits over plant cellulose due to distinctive features, e.g. higher polymerization degree and purity, superior crystalline structure, water retention, biocompatibility, and biodegradability, which make BC a valuable material in creating sustainable and innovative solutions across various industries. However, the high operating cost, expensive culture media components and low productivity often limits its widespread industrial usage. In our study, BC production by Gluconacetobacter liquefaciens MTCC 3135 was analyzed under agitation and static cultivation in Hestrin–Schramm (HS) medium. Variations were observed in microbial growth kinetics parameters of G. liquefaciens under agitation and static cultivation. Highest BC yield at 3.55 ± 0.26 g/L was obtained in static cultivation, while agitated culture condition yielded the lowest BC at 2.59 ± 0.16 g/L. BC samples produced under agitation and static culture were purified using NaOH treatment and were subjected to physicochemical characterization using FTIR, XRD and DSC. FTIR spectra showed peak shifting and variations in peak intensities for BC samples produced under agitation and static culture, while compared to commercial cellulose (Himedia, India). BC produced under static culture was more crystalline compared to BC samples produced under agitation, as determined by FTIR and XRD. The research contributes valuable insights into alternative sources of BC production, targeting to fill gaps in knowledge and promote sustainable cellulose production

Keywords: Bacterial cellulose, Gluconacetobacter liquefaciens, Static, Agitation, FTIR, XRD, DSC

How to cite this article: Garima Singh, Pammi Gauba, Garima Mathur. Influence of Agitation and Static Cultivation on Physicochemical Characteristics of Bacterial Cellulose Produced by Gluconacetobacter liquefaciens MTCC 3135. Research & Reviews : A Journal of Biotechnology. 2024; ():-.
How to cite this URL: Garima Singh, Pammi Gauba, Garima Mathur. Influence of Agitation and Static Cultivation on Physicochemical Characteristics of Bacterial Cellulose Produced by Gluconacetobacter liquefaciens MTCC 3135. Research & Reviews : A Journal of Biotechnology. 2024; ():-. Available from: https://journals.stmjournals.com/rrjobt/article=2024/view=145768


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Received March 28, 2024
Accepted April 8, 2024
Published May 10, 2024
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