Characterization of cellulose producing bacterial isolates from rotten fruits

Year : 2024 | Volume :14 | Issue : 01 | Page : 38-45
By

Samriddh Srivastava

Garima Mathur

  1. Research Scholar Department of Biotechnology, Jaypee Institute of Information Technology Uttar Pradesh India
  2. Assistant Professor Department of Biotechnology, Jaypee Institute of Information Technology Uttar Pradesh India

Abstract

Cellulose, the most abundant natural polymer, is predominantly sourced from plant wood but can also be synthesized by certain bacteria in the form of bio-cellulose. The potential applications of bio-cellulose are extensive, particularly in the biomedical field for tissue engineering, drug delivery, and more. The distinct properties and purity of bacterial cellulose, in contrast to plant-derived cellulose, underscore its importance and drive further research in the area. This study focuses on bacterial strains isolated from spoiled fruits to explore their cellulose-producing capabilities for broader industrial applications. Spoiled fruits were collected from NOIDA, India, and bacterial isolation was carried out from samples of apple, grape, banana, as well as coconut. The isolated strains were cultured in Hestrin- Schramm medium and subsequently identified using morphological, biochemical, and 16S rRNA sequencing techniques. The findings obtained from this study revealed variations in cellulose production among the isolated strains. Out of the ten bacterial isolates obtained, each fruit type contributed differently to the total number of isolates. Through the identification process, several isolates were classified as belonging to the Komagataeibacter species. Specific isolates such as BC-G1 and BC-C1, demonstrating early promising cellulose production, were further characterized via 16S rRNA sequencing, confirming their high similarity to known Komagataeibacter saccharivorans strains. Additionally, this study further utilized FTIR spectroscopy to examine the chemical properties of the produced cellulose, providing insights into its structural characteristics and potential applications. The findings from this study highlight the diversity of cellulose-producing bacterial strains isolated from spoiled fruits and their potential for industrial utilization in various sectors, including biomedicine and materials science.

Keywords: Cellulose, Komagataeibacter, Rotten Fruits, Hestrin- Schramm medium, FTIR, SEM

[This article belongs to Research & Reviews : A Journal of Biotechnology(rrjobt)]

How to cite this article: Samriddh Srivastava, Garima Mathur. Characterization of cellulose producing bacterial isolates from rotten fruits. Research & Reviews : A Journal of Biotechnology. 2024; 14(01):38-45.
How to cite this URL: Samriddh Srivastava, Garima Mathur. Characterization of cellulose producing bacterial isolates from rotten fruits. Research & Reviews : A Journal of Biotechnology. 2024; 14(01):38-45. Available from: https://journals.stmjournals.com/rrjobt/article=2024/view=141159


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References

  1. Parveen S, Bhat I U, Khanam Z, Rak A E, Yusoff H M and Akhter M S. Phytoremediation: In situ Alternative for Pollutant Removal from Contaminated Natural Media: A Brief Review. Biointerface Research in Applied Chemistry. 2021 Oct 17;12(4):4945–60. https://doi.org/10.33263/briac124.49454960
  2. Nunes SP, Çulfaz-Emecen PZ, Ramon G, Visser T, Koops GH, Jin W, et al. Thinking the future of membranes: Perspectives for advanced and new membrane materials and manufacturing processes. Journal of Membrane Science.2020 Mar 1; 598:117761. https://doi.org/10.1016/j.memsci.2019.117761
  3. Krystynowicz A, Czaja W, Wiktorowska-Jezierska A, Gonçalves-Miśkiewicz M, Turkiewicz M, Bielecki S. Factors affecting the yield and properties of bacterial cellulose. Journal of Industrial Microbiology and Biotechnology. 2002 Oct 1;29(4):189–95. https://doi.org/10.1038/sj.jim.7000303
  4. Gorgieva S, Trček J. Bacterial cellulose: Production, modification and perspectives in biomedical applications. Nanomaterials.2019 Sep 20;9(10):1352. https://doi.org/10.3390/nano9101352
  5. Srivastava S, Mathur G. Bacterial cellulose: a multipurpose biomaterial for manmade world. Current Applied Science and Technology.2022 Oct 27;23(3). https://doi.org/10.55003/cast.2022.03.23.014
  6. Urbina L, Corcuera MÁ, Gabilondo N, Eceiza A, Retegi A. A review of bacterial cellulose: sustainable production from agricultural waste and applications in various fields. Cellulose. 2021 Jul 10;28(13):8229–53. https://doi.org/10.1007/s10570-021-04020-4
  7. Pogorelova N, Rogachev E, Digel I, Chernigova S and Nardin D. Bacterial cellulose nanocomposites: morphology and mechanical properties. Materials. 2020 Jun 25;13(12):2849. https://doi.org/10.3390/ma13122849
  8. Swingler S, Gupta A, Gibson H, Kowalczuk M, Heaselgrave W, Radecka I. Recent advances and applications of bacterial cellulose in biomedicine. Polymers. 2021 Jan 28;13(3):412. https://doi.org/10.3390/polym13030412
  9. Dutt MA, Hanif MA, Nadeem F, Bhatti HN. A review of advances in engineered composite materials popular for wastewater treatment. Journal of Environmental Chemical Engineering. 2020 Oct 1;8(5):104073. https://doi.org/10.1016/j.jece.2020.104073
  10. Schramm M., Hestrin S., Factors affecting production of cellulose at the air/liquid interface of a culture of Acetobacter xylinum. Microbiology.1954, 11:123.
  11. Srivastava S, Mathur G. Komagataeibacter saccharivorans strain BC-G1: an alternative strain for production of bacterial cellulose. Biologia. 2022 Nov 10;77(12):3657–68. https://doi.org/10.1007/s11756-022-01222-4
  12. Lavasani PS, Motevaseli E, Shirzad M, Modarressi MH. Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegar and molecular analyses. Iranian Journal of Microbiology. 2017 Dec 1;9(6):338–47. https://pubmed.ncbi.nlm.nih.gov/29487732
  13. Liu M, Liu LP, Jia S, Li S, Zou Y, Zhong C. Complete genome analysis of Gluconacetobacter xylinus CGMCC 2955 for elucidating bacterial cellulose biosynthesis and metabolic regulation. Scientific Reports. 2018 Apr 19;8(1).https://doi.org/10.1038/s41598-018-24559-wv
  14. Voon WWY, Rukayadi Y, Hussin ASM. Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits. Letters in Applied Microbiology.2016 Apr 13;62(5):428–33. https://doi.org/10.1111/lam.12568
  15. Rangaswamy BE, Vanitha KP, Hungund BS. Microbial cellulose production from bacteria isolated from rotten fruit. International Journal of Polymer Science. 2015 Jan 1; 2015:1–8. https://doi.org/10.1155/2015/280784
  16. Ibrahim S.A., Abd-El-Aal S.K., AG A., ElSayd M.A. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2023 Mar 1;6(3). https://doi.org/10.26655/jmchemsci.2023.3.19
  17. Yanti NA, Ahmad SW, Ambardini S, Muhiddin NH, Sulaiman LOI. Screening of acetic acid bacteria from pineapple waste for bacterial cellulose production using sago liquid waste. Biosaintifika: Journal of Biology & Biology Education.2017 Dec 31;9(3):387.https://doi.org/10.15294/biosaintifika.v9i3.10241
  18. Andritsou V, De Melo EM, Tsouko E, Ladakis D, Maragkoudaki S, Koutinas A, et al. Synthesis and characterization of bacterial cellulose from citrus-based sustainable resources. ACS Omega.2018 Aug 31;3(8):10365–73. https://doi.org/10.1021/acsomega.8b01315
  19. Raiszadeh-Jahromi Y, Bari MR, Almasi H, Amiri S. Optimization of bacterial cellulose production by Komagataeibacter xylinus PTCC 1734 in a low-cost medium using optimal combined design. Journal of Food Science and Technology.2020 Feb 3;57(7):2524–33. https://doi.org/10.1007/s13197-020-04289-6
Regular Issue Subscription Original Research
Volume 14
Issue 01
Received March 28, 2024
Accepted April 8, 2024
Published April 19, 2024
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