Screening of Pharmaceutically Significant Methioninase-Producing Fungi from the Satpura Range of Hoshangabad District and Its Large-Scale Production

Year : 2025 | Volume : 12 | Issue : 02 | Page : 33 43
    By

    Apurva Munje,

  • Umesh Kumar Dhurwe,

  • Sunil Kumar Snehi,

  1. PhD Research Scholar, Department of Microbiology, Barkatullah University, Bhopal, Madhya Pradesh, India
  2. Principal, Department of Microbiology, Government College, Seoni Malwa, Madhya Pradesh, India
  3. Assistant Professor, Department of Microbiology, Barkatullah University, Bhopal, Madhya Pradesh, India

Abstract

L-methioninase, an enzyme with notable anticancer properties, has emerged as a promising therapeutic agent due to its ability to selectively degrade methionine, a critical amino acid for the survival of methionine-dependent cancer cells. Methionine dependency, observed in many tumor cells, is a metabolic vulnerability that can be exploited for targeted cancer therapy. The Satpura Range in the Narmadapuram District, India, with its diverse microbial ecosystems, offers a rich reservoir for the discovery of methioninase-producing fungi. This study aimed to systematically explore the fungal biodiversity of this region to identify potent methioninase producers, optimize enzyme production, and evaluate their potential for large-scale synthesis. Soil and organic matter samples were collected from four distinct ecological locations, and fungal isolates were screened for methioninase activity using selective culture media containing methionine as the sole carbon source. Promising isolates were subjected to morphological, microscopic, and molecular identification to ensure accurate taxonomy. Key production parameters, including temperature, pH, carbon and nitrogen sources, and trace elements, were optimized to enhance enzyme yield. Large-scale production was achieved through liquid-state fermentation, yielding significant quantities of methioninase with high purity as confirmed by SDS-PAGE analysis. The findings underscore the untapped potential of Satpura’s microbial diversity in pharmaceutical enzyme research and establish a foundation for the cost-effective and sustainable production of methioninase for therapeutic applications. This study paves the way for future research into the clinical efficacy of methioninase-based cancer therapies, contributing to advancements in enzyme biotechnology and cancer treatment.

Keywords: Methioninase, Indigenous fungal isolates, Liquid-state fermentation, Cancer therapy, Personalized medicine, Enzyme characterization

[This article belongs to Research & Reviews: A Journal of Bioinformatics ]

How to cite this article:
Apurva Munje, Umesh Kumar Dhurwe, Sunil Kumar Snehi. Screening of Pharmaceutically Significant Methioninase-Producing Fungi from the Satpura Range of Hoshangabad District and Its Large-Scale Production. Research & Reviews: A Journal of Bioinformatics. 2025; 12(02):33-43.
How to cite this URL:
Apurva Munje, Umesh Kumar Dhurwe, Sunil Kumar Snehi. Screening of Pharmaceutically Significant Methioninase-Producing Fungi from the Satpura Range of Hoshangabad District and Its Large-Scale Production. Research & Reviews: A Journal of Bioinformatics. 2025; 12(02):33-43. Available from: https://journals.stmjournals.com/rrjobi/article=2025/view=203653


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Regular Issue Subscription Original Research
Volume 12
Issue 02
Received 29/01/2025
Accepted 31/01/2025
Published 17/03/2025
Publication Time 47 Days
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