Mamdouh Alshammari,
- Assistant Professor, Department of Biology, College of Science, University of hail, Hail, Saudi Arabia
Abstract
Effective wastewater treatment through green and sustainable approach is one of the primaries concerning issue of the Kingdom of Saudi Arabia (KSA) to resolve water scarcity. KSA has witnessed massive industrial development over the last four decades alongwith majorly generate wastewater pollution. Therefore, sustainable wastewater treatment is among primary research goal of KSA and current study is also targeted in this direction. Henceforth, The objective of the present study is to treat KSA wastewater samples using a green, sustainable, and renewable approach, by leveraging a composite treatment based on iron‐oxide nanoparticles and polymer-enzyme conjugates. In present study iron-oxide nanoparticles, synthesized via a green method, were conjugated with lignocellulolytic enzymes. These enzymes were produced by solid-state fermentation (SSF) of a polymeric, nature-based dry sludge feedstock. Three wastewater samples collected from KSA have been characterized before and after treatment and characterizations include color change, pH, BOD, COD and TS. These factors showed significant reduction after treatment. Additionally, Maximum enzyme 47 U/mL laccasse, 30 U/L FP, 59 U/mL EG and 67 U/mL BGL were produced in optimum 96 h of incubation of SSF at 4 g concentration of substrate. Additionally, effective biodegradation of wastewater samples were recorded at different concentration of Fe-En composite. Further, at optimum 3% of Fe-En Composite, 97%, 98% and 98% pollutants degradation were recorded in 48h. This research work shows promising potential in wastewater treatment of KSA.
Keywords: Green synthesis; renewable method; nanoparticle; polymer; KSA; wastewater treatment; Nanocomposite
[This article belongs to Journal of Polymer and Composites ]
Mamdouh Alshammari. Green Renewable Synthesis of Iron Oxide Nanoparticles Embedded in a Polymer-Enzyme Composite for Wastewater Treatment in Hail, Saudi Arabia. Journal of Polymer and Composites. 2025; 13(06):297-305.
Mamdouh Alshammari. Green Renewable Synthesis of Iron Oxide Nanoparticles Embedded in a Polymer-Enzyme Composite for Wastewater Treatment in Hail, Saudi Arabia. Journal of Polymer and Composites. 2025; 13(06):297-305. Available from: https://journals.stmjournals.com/jopc/article=2025/view=232852
Browse Figures
References
- Yaqoob AA, Parveen T, Umar K, Mohamad Ibrahim MN. Role of nanomaterials in the treatment of wastewater: A review. Water. 2020 Feb 12;12(2):495.
- Paramasivam G, Palem VV, Sundaram T, Sundaram V, Kishore SC, Bellucci S. Nanomaterials: Synthesis and applications in theranostics. Nanomaterials. 2021 Nov 28;11(12):3228.
- Hassan, S.E.D.; Fouda, A.; Saied, E.; Farag, M.M.S.; Eid, A.M.; Barghoth, M.G.; Awad, M.A.; Hamza, M.F.; Awad, M.F. Rhizopus Oryzae-Mediated Green Synthesis of Magnesium Oxide Nanoparticles (MgO-NPs): A Promising Tool for Antimicrobial, Mosquitocidal Action, and Tanning Effluent Treatment. J. Fungi 2021, 7, 372.
- Masroor S, Kumar A, Goswami A, Krishan H, Hussain S, Khan ME. Sustainable Approaches Towards Nanomaterial Synthesis: Strategies, Properties, and Process Integration. Sustainable Processes Connect. 2025 Sep 2;1(1):1-0.
- Prudente ES, dos Santos MM, Pantoja GV, Bezerra FW, Komesu A, Fontanari GG, Martins LH. Sustainable Valorization of Amazonian Pracaxi (Pentaclethra macroloba (Willd.)) Residues via Optimized Ultrasound-Assisted Extraction of Bioactive Compounds. Sustainable Processes Connect. 2025 Sep 22;1(1):1-0.).
- Chatterji T, Singh T, Khanna N, Bhagat T, Tyagi D, Totlani R. Microbial Bioremediation Strategies for Sustainable Wastewater Treatment. Sustainable Processes Connect. 2025 Sep 20;1(1):1-0.
- Kumar A, Devnani GL, Pal DB. Sustainable Degradation of Organic Pollutants Using Various Treatment Processes: A Review. Sustainable Processes Connect. 2025 Oct 27;1(1):1-0.
- Ajinkya N, Yu X, Kaithal P, Luo H, Somani P, Ramakrishna S. Magnetic iron oxide nanoparticle (IONP) synthesis to applications: present and future. Materials. 2020 Oct 18;13(20):4644.
- Devi HS, Boda MA, Shah MA, Parveen S, Wani AH. Green synthesis of iron oxide nanoparticles using Platanus orientalis leaf extract for antifungal activity. Green Processing and Synthesis. 2019 Jan 1;8(1):38-45.
- Niraimathee VA, Subha V, Ravindran RE, Renganathan S. Green synthesis of iron oxide nanoparticles from Mimosa pudica root extract. International Journal of Environment and Sustainable Development. 2016;15(3):227-40.
- Liu JF, Jang B, Issadore D, Tsourkas A. Use of magnetic fields and nanoparticles to trigger drug release and improve tumor targeting. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology. 2019 Nov;11(6):e1571.
- Kirdat PN, Dandge PB, Hagwane RM, Nikam AS, Mahadik SP, Jirange ST. Synthesis and characterization of ginger (Z. officinale) extract mediated iron oxide nanoparticles and its antibacterial activity. Materials Today: Proceedings. 2021 Jan 1;43:2826-31.
- Badmapriya D, Asharani IV. Dye degradation studies catalysed by green synthesized iron oxide nanoparticles. International Journal of ChemTech Research. 2016;9(6):409-16..
- Katata-Seru L, Moremedi T, Aremu OS, Bahadur I. Green synthesis of iron nanoparticles using Moringa oleifera extracts and their applications: Removal of nitrate from water and antibacterial activity against Escherichia coli. Journal of Molecular Liquids. 2018 Apr 15;256:296-304.
- Mohammed MK, Khudhair SH, Jabber AD. Production, purification, and characterization of laccase enzyme from local isolate Bacillus cereus TY10 isolated from soil samples. J. Nat. Sci. Biol. Med. 2024;15:90-6.
- Ghose TK. Measurement of cellulase activities. Pure and applied Chemistry. 1987 Jan 1;59(2):257-68.
- Kubicek CP. β-Glucosidase excretion by Trichoderma pseudokoningii: correlation with cell wall bound β-1.3-glucanase activities. Archives of Microbiology. 1982 Oct;132(4):349-54.
- Toan VP, Vinh MP, Dung VN, Jiro K. Treatment efficiency of a combination of alternative technologies in removing pollutants from pesticide containing wastewater. Environ Eng Res. 2021;26(6):200263. doi:10.4491/eer.2020.263.]
- Utari AW, Herdiansyah H. Filtration as a water treatment method: Used to remove TSS and COD in household wastewater. InAIP Conference Proceedings 2020 Jul 8 (Vol. 2245, No. 1, p. 060004). AIP Publishing LLC.
- Sidhu GK, Singh S, Kumar V, Dhanjal DS, Datta S, Singh J. Toxicity, monitoring and biodegradation of organophosphate pesticides: a review. Critical reviews in environmental science and technology. 2019 Jul 3;49(13):1135-87.
- Annadurai Y, Balasubramanian B, Arumugam VA, Liu W, Pushparaj K, Pappusamy M, Bhotla HK, Meyyazhagan A, Easwaran M, Piramanayagam S. Comprehensive strategies of Lignocellulolytic enzyme production from microbes and their applications in various commercial-scale faculties.
- Civzele A, Stipniece-Jekimova AA, Mezule L. Biodegradation of screenings from sewage treatment by white rot fungi. Fungal Biology and Biotechnology. 2025 May 14;12(1):7.
- Rana P, Inbaraj BS, Gurumayum S, Sridhar K. Sustainable production of lignocellulolytic enzymes in solid-state fermentation of agro-industrial waste: Application in pumpkin (cucurbita maxima) juice clarification. Agronomy. 2021 Nov 24;11(12):2379.
- Lin R, Li Y, Yong T, Cao W, Wu J, Shen Y. Synergistic effects of oxidation, coagulation and adsorption in the integrated fenton-based process for wastewater treatment: A review. Journal of Environmental Management. 2022 Mar 15;306:114460.
- Al-Jassim N, Ansari MI, Harb M, Hong PY. Removal of bacterial contaminants and antibiotic resistance genes by conventional wastewater treatment processes in Saudi Arabia: Is the treated wastewater safe to reuse for agricultural irrigation?. Water research. 2015 Apr 15;73:277-90.
- Haneef T, Mustafa MR, Wan Yusof K, Isa MH, Bashir MJ, Ahmad M, Zafar M. Removal of polycyclic aromatic hydrocarbons (PAHs) from produced water by ferrate (VI) oxidation. Water. 2020 Nov 9;12(11):3132.
- Dong H, Jiang Z, Zhang C, Deng J, Hou K, Cheng Y, Zhang L, Zeng G. Removal of tetracycline by Fe/Ni bimetallic nanoparticles in aqueous solution. Journal of colloid and interface science. 2018 Mar 1;513:117-25.
- Alhajri MF, Jamil R, Abdalla EM, Alqahtany A, Almazroua S, Al-Gehlani WA. Status of wastewater treatment and reuse in Saudi Arabia and its forecasting for the year 2035. Applied Water Science. 2025 Jul;15(7):167.
- Dawoud MA, Ewea HA, Alaswad SO. The future of wastewater treatment and reuse in Kingdom of Saudi Arabia. Desalination and Water Treatment. 2022 Jul 1;263:127-38.
- Ouda OK. Treated wastewater use in Saudi Arabia: challenges and initiatives. International Journal of Water Resources Development. 2016 Sep 2;32(5):799-809.

Journal of Polymer & Composites
| Volume | 13 |
| Issue | 06 |
| Received | 15/11/2025 |
| Accepted | 19/11/2025 |
| Published | 25/11/2025 |
| Publication Time | 10 Days |
Login
PlumX Metrics