Stimulation of Plant Growth Promoting Rhizobacteria with ZnO Nanoparticles to Improve Growth and Development of Groundnut Plants (Arachis Hypogaea L.)

Open Access

Year : 2024 | Volume :12 | Special Issue : 06 | Page : 253-269
By
vector

Jahal Dangar,

vector

Gunja Vasant,

vector

Shweta Bhatt,

vector

Ragini Raghav,

  1. Research Scholar, Department of Biotechnology, Atmiya University, Rajkot, Gujarat, India
  2. Research Scholar, Department of Microbiology, Atmiya University, Rajkot, Gujarat, India
  3. Assistant Professor, Department of Biotechnology, Atmiya University, Rajkot, Gujarat, India
  4. Assistant Professor, Department of Biotechnology, Atmiya University, Rajkot, Gujarat, India

Abstract document.addEventListener(‘DOMContentLoaded’,function(){frmFrontForm.scrollToID(‘frm_container_abs_126845’);});Edit Abstract & Keyword

Rhizobacteria that stimulate plant growth have been extensively utilized as biofertilizers to improve the nutrient content of the soil for various crop plants. In the present study, we have isolated plant growth promoting rhizobacteria (PGPR) for rhizospheric soil of groundnut crops from Gujarat agricultural fields in the Saurashtra region. Fifty-two isolates with particular colony characteristics were obtained from rhizospheric soil. All strain was tested for their plant growth promoting (PGP) traits including chitinase assay, phosphate and potassium solubilisation, nitrification, zinc solubilization, indole acetic acid (IAA), ammonia and hydrogen cyanide (HCN) production, gibberellins production and siderophore production in order to explore its potential for improving plant growth. Only three isolates displayed all PGP traits positive and one of the potent isolate was identified using molecular identification. The strain RG12 has close evolutionary similarity with Bacillus haynesii. Furthermore, zinc oxide nanoparticles (ZnO NPs) were synthesized using sol gel method. The nanoparticles were characterized using UV visible spectrophotometer, scanning electron microscope (SEM) and transmission electron microscope (TEM). The ZnO NPs concentrations (100 to 800 ppm) were used evaluate the optimized concentration with Bacillus haynesii. The pot assay indicated that the combined application of RG12 and ZnO NPs (400 ppm) resulted in highest growth and development of groundnut plants in comparison to all other individual treatments.

Keywords: PGP traits, Bacillus haynesii, ZnO nanoparticles, optimization of ZnO NPs with PGPR (pot assay).

[This article belongs to Special Issue under section in Journal of Polymer and Composites (jopc)]

How to cite this article:
Jahal Dangar, Gunja Vasant, Shweta Bhatt, Ragini Raghav. Stimulation of Plant Growth Promoting Rhizobacteria with ZnO Nanoparticles to Improve Growth and Development of Groundnut Plants (Arachis Hypogaea L.). Journal of Polymer and Composites. 2024; 12(06):253-269.
How to cite this URL:
Jahal Dangar, Gunja Vasant, Shweta Bhatt, Ragini Raghav. Stimulation of Plant Growth Promoting Rhizobacteria with ZnO Nanoparticles to Improve Growth and Development of Groundnut Plants (Arachis Hypogaea L.). Journal of Polymer and Composites. 2024; 12(06):253-269. Available from: https://journals.stmjournals.com/jopc/article=2024/view=0


Full Text PDF

Browse Figures

References
document.addEventListener(‘DOMContentLoaded’,function(){frmFrontForm.scrollToID(‘frm_container_ref_126845’);});Edit

  1. Dimitrijević MS. Technological progress in the function of productivity and sustainability of agriculture: The case of innovative countries and the Republic of Serbia. Journal of Agriculture and Food Research. 2023 Dec 1;14:100856.
  2. Nwachukwu BC, Babalola OO, Hassen AI. Rhizosphere competence and applications of plant growth-promoting rhizobacteria in food production-a review. Scientific African. 2024 Jan 11:e02081.
  3. Rabari KV, Patel JM, Sundesha DL. Soil fertility status: As affected by micronutrient application in groundnut.
  4. Baweja P, Kumar S, Kumar G. Fertilizers and pesticides: Their impact on soil health and environment. Soil health. 2020:265–85.
  5. Hossain MM, Sultana F, Khan S, Nayeema J, Mostafa M, Ferdus H, Tran LS, Mostofa MG. Carrageenans as biostimulants and bio-elicitors: plant growth and defense responses. Stress Biology. 2024 Jan 3;4(1):3.
  6. Etesami H, Adl SM. Plant growth-promoting rhizobacteria (PGPR) and their action mechanisms in availability of nutrients to plants. Phyto-Microbiome in stress regulation. 2020:147–203.
  7. El-Saadony MT, Saad AM, Soliman SM, Salem HM, Desoky ES, Babalghith AO, El-Tahan AM, Ibrahim OM, Ebrahim AA, Abd El-Mageed TA, Elrys AS. Role of nanoparticles in enhancing crop tolerance to abiotic stress: A comprehensive review. Frontiers in plant science. 2022 Nov 2;13:946717.
  8. Hussain F, Hadi F, Rongliang Q. Effects of zinc oxide nanoparticles on antioxidants, chlorophyll contents, and proline in Persicaria hydropiper L. and its potential for Pb phytoremediation. Environmental Science and Pollution Research. 2021 Jul;28(26):34697–713.
  9. Gordon SA, Paleg LG. Quantitative measurement of indole acetic acid. Physiol Plant. 1957;10:37–48.
  10. Cappuccino JG, Sherman N. Microbiology: a laboratory manual. Pearson Higher Ed; 2013 Feb 20.
  11. Alström S, Burns RG. Cyanide production by rhizobacteria as a possible mechanism of plant growth inhibition. Biology and Fertility of Soils. 1989 Sep;7:232–8.
  12. Sehrawat A, Sindhu SS, Glick BR. Hydrogen cyanide production by soil bacteria: Biological control of pests and promotion of plant growth in sustainable agriculture. Pedosphere. 2022 Feb 1;32(1):15–38.
  13. Vasant G, Bhatt S, Raghav R. Isolation and Molecular Characterization of Plant Growth Promoting Rhizobacteria from Groundnut (Arachis Hypogaea L.) Rhizosphere. Current Agriculture Research Journal. 2023 Apr 1;11(1).
  14. Thakur A, Parikh S. Isolation and characterization of phosphate solubilizing bacteria associated with groundnut rhizosphere. International Journal of Agricultural Science and Research (IJASR) Vol. 2017;6.
  15. Zhang C, Kong F. Isolation and identification of potassium-solubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants. Applied soil ecology. 2014 Oct 1;82:18–25.
  16. Mathivanan, S., Chidambaram, A. A., Sundramoorthy, P., Baskaran, L., and Kalaikandhan, R. (2014). Effect of combined inoculations of Plant Growth Promoting Rhizobacteria (PGPR) on the growth and yield of groundnut (Arachis hypogaea L.). International Journal of Current Microbiology and Applied Sciences, 3(8), 1010–1020.
  17. Jackson ML. Soil chemical analysis, pentice hall of India Pvt. Ltd., New Delhi, India. 1973;498:151–4.
  18. Manasa SG, Naik NM, Ramesh Y, Gundappagol RC. In vitro screening of the isolates for zinc solubilization and growth promoting attributes. Journal of Pharmacognosy and Phytochemistry. 2019;8(5):1205–9.
  19. Schwyn B, Neilands JB. Universal chemical assay for the detection and determination of siderophores. Analytical biochemistry. 1987 Jan 1;160(1):47–56.
  20. Arnon DI. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant physiology. 1949 Jan;24(1):1.
  21. Gnanasangeetha D, SaralaThambavani D. One pot synthesis of zinc oxide nanoparticles via chemical and green method. Res J Mater Sci. 2013;2320:6055.
  22. Guardiola-Márquez CE, López-Mena ER, Segura-Jiménez ME, Gutierrez-Marmolejo I, Flores-Matzumiya MA, Mora-Godínez S, Hernández-Brenes C, Jacobo-Velázquez DA. Development and evaluation of zinc and iron nanoparticles functionalized with plant growth-promoting rhizobacteria (PGPR) and microalgae for their application as bio-nanofertilizers. Plants. 2023 Oct 23;12(20):3657.
  23. Zand AD, Mikaeili Tabrizi A, Vaezi Heir A. Application of titanium dioxide nanoparticles to promote phytoremediation of Cd-polluted soil: contribution of PGPR inoculation. Bioremediation Journal. 2020 Jul 2;24(2-3):171–89.
  24. Doddavarapu B, Crasta GL, Shankar M. Comparative studies on chlorophyll concentration in some important plant families. Journal of Pharmacognosy and Phytochemistry. 2021;10(3):214–20.
  25. Zand AD, Mikaeili Tabrizi A, Vaezi Heir A. Application of titanium dioxide nanoparticles to promote phytoremediation of Cd-polluted soil: contribution of PGPR inoculation. Bioremediation Journal. 2020 Jul 2;24(2-3):171–89.
  26. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food chemistry. 1999 Mar 1;64(4):555–9.
  27. Siddhuraju P, Becker K. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. Journal of agricultural and food chemistry. 2003 Apr 9;51(8):2144–55.
  28. Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant and soil. 1973 Aug;39:205–7.
  29. Ábrahám E, Hourton-Cabassa C, Erdei L, Szabados L. Methods for determination of proline in plants. Plant stress tolerance: methods and protocols. 2010:317–31.
  30. Nawaz S, Bano A. Effects of PGPR (Pseudomonas sp.) and Ag-nanoparticles on enzymatic activity and physiology of cucumber. Recent patents on food, nutrition and agriculture. 2020 Aug 1;11(2):124–36
  31. Bonjoch NP, Tamayo PR. Protein content quantification by Bradford method. InHandbook of plant ecophysiology techniques 2001 Jun 30 (pp. 283–295). Dordrecht: Springer Netherlands.
  32. Kumar A, Radhakrishnan EK, editors. Microbial Endophytes: Functional Biology and Applications. Woodhead Publishing; 2020 Mar 5.
  33. Bhatt S, Pandhi N, Raghav R. Improved salt tolerance and growth parameters of groundnut (Arachis hypogaea L.) employing Halotolerant Bacillus cereus SVSCD1 isolated from Saurashtra Region, Gujarat. Gujarat. Ecol. Environ. Cos. 2020;26:S199–212.
  34. Verma P, Yadav AN, Khannam KS, Saxena AK, Suman A. Potassium-solubilizing microbes: diversity, distribution, and role in plant growth promotion. Microorganisms for green revolution: Volume 1: Microbes for sustainable crop production. 2017:125–49.
  35. Sindhu SS, Parmar P, Phour M, Sehrawat A. Potassium-solubilizing microorganisms (KSMs) and its effect on plant growth improvement. Potassium solubilizing microorganisms for sustainable agriculture. 2016:171–85.
  36. Devi R, Thakur R. Screening and identification of bacteria for plant growth promoting traits from termite mound soil. Journal of Pharmacognosy and Phytochemistry. 2018;7(2):1681–6.
  37. Sosnowski J, Truba M, Vasileva V. The impact of auxin and cytokinin on the growth and development of selected crops. Agriculture. 2023 Mar 21;13(3):724.
  38. Goswami D, Vaghela H, Parmar S, Dhandhukia P, Thakker JN. Plant growth promoting potentials of Pseudomonas spp. strain OG isolated from marine water. Journal of plant interactions. 2013 Dec 1;8(4):281–90.
  39. Anwar S, Ali B, Sajid I. Screening of rhizospheric actinomycetes for various in-vitro and in-vivo plant growth promoting (PGP) traits and for agroactive compounds. Frontiers in microbiology. 2016 Aug 29;7:203732.
  40. Gyaneshwar P, Kumar GN, Parekh LJ. Effect of buffering on the phosphate-solubilizing ability of microorganisms. World Journal of Microbiology and Biotechnology. 1998 Oct;14:669–73.
  41. Sahu S, Rajbonshi MP, Gujre N, Gupta MK, Shelke RG, Ghose A, Rangan L, Pakshirajan K, Mitra S. Bacterial strains found in the soils of a municipal solid waste dumping site facilitated phosphate solubilization along with cadmium remediation. Chemosphere. 2022 Jan 1;287:132320.
  42. Lenin G, Jayanthi M. Indole acetic acid, gibberellic acid and siderophore production by PGPR isolates from rhizosphericsoils of Catharanthus roseus. Int J Pharmacy Biol Arch. 2012;3(4):933–8.
  43. ALKahtani MD, Attia KA, Hafez YM, Khan N, Eid AM, Ali MA, Abdelaal KA. Chlorophyll fluorescence parameters and antioxidant defense system can display salt tolerance of salt acclimated sweet pepper plants treated with chitosan and plant growth promoting rhizobacteria. Agronomy. 2020 Aug 12;10(8):1180. 1180.
  44. Sharma IP, Sharma AK. Physiological and biochemical changes in tomato cultivar PT-3 with dual inoculation of mycorrhiza and PGPR against root-knot nematode. Symbiosis. 2017 Mar;71(3):175–83.
  45. Rezazadeh S, Ilkaee M, Aghayari F, Paknejad F, Rezaee M. The physiological and biochemical responses of directly seeded and transplanted maize (Zea mays L.) supplied with plant growth-promoting rhizobacteria (PGPR) under water stress. Iranian Journal of Plant Physiology. 2019 Oct 1;10(1):3009–21.
  46. Liu H, Wang X, Wang D, Zou Z, Liang Z. Effect of drought stress on growth and accumulation of active constituents in Salvia miltiorrhiza Bunge. Industrial Crops and Products. 2011 Jan 1;33(1):84–8.
  47. Liu M, Li X, Liu Y, Cao B. Regulation of flavanone 3-hydroxylase gene involved in the flavonoid biosynthesis pathway in response to UV-B radiation and drought stress in the desert plant, Reaumuriasoongorica. Plant physiology and biochemistry. 2013 Dec 1;73:161–7.
  48. Sardar R, Ahmed S, Shah AA, Yasin NA. Selenium nanoparticles reduced cadmium uptake, regulated nutritional homeostasis and antioxidative system in Coriandrum sativum grown in cadmium toxic conditions. Chemosphere. 2022 Jan 1;287:132332.
  49. Stefan MA, Munteanu N, Stoleru V, Mihasan M. Effects of inoculation with plant growth promoting rhizobacteria on photosynthesis, antioxidant status and yield of runner bean. Romanian Biotechnological Letters. 2013 Mar 1;18(2):8132–43.
  50. Mishra BK, Sharma A, Aishwath OP, Sharma YK, Kant K, Vishal MK, Saxena SN, Ranjan JK. Microbiological profile of coriander (Coriandrum sativum L.) crop rhizosphere in Rajasthan and screening for auxin producing rhizobacteria. International Journal of Seed Spices. 2013 Jul;3(2):59–64.

 

Special Issue Open Access Original Research
Volume 12
Special Issue 06
Received 30/04/2024
Accepted 16/07/2024
Published 09/12/2024
185