Recent Advancements in High-Performance Liquid Chromatography: A Comparative Approach

Open Access

Year : 2025 | Volume : 13 | Special Issue 01 | Page : 40 48
    By

    Aayushi Agarwal Bansal,

  • Samixa Patel,

  1. PhD Research Scholar, Department of Pharmacy, School of Pharmaceutical Sciences,Atmiya University, Rajkot, Gujarat, India
  2. Associate Professor, Department of Pharmacy, School of Pharmaceutical Sciences,Atmiya University, Rajkot, Gujarat, India

Abstract

Modern separation science relies heavily on chromatography, which is used in all research institutes and pharmaceutical industries all around the globe. Of all the chromatography approaches, HPLC i.e. high performance liquid chromatography is the one that is most frequently used analytically. Recent improvements in chromatographic supports and liquid chromatography (LC) equipment have made rapid and incredibly effective separations possible. This summary emphasizes the key milestones and foundational advances in HPLC instrumentation during the last few decades. It provides an overview of recent advancements in HPLC, including as UFLC, RRLC, UPLC, and Nano LC, along with their uses. This article mainly contrasts recent developments in HPLC with regard to instrumental operational parameters such injection volume application, flow rate, and column temperature, as well as benefits of each approach over HPLC. Pharmaceutical and biological materials are assessed using HPLC technology, which is important for both quantitative and qualitative analysis. It is the most rapid, versatile, and secures chromatographic analysis technique known for drug component quality control. It is anticipated that the capabilities of High-Performance Liquid Chromatography (HPLC) would be further expanded by forthcoming improvements. Two significant advancements are the equipment’s miniaturization, which will enable more efficient and compact systems, and its integration with mass spectrometry, which will increase the analytical capabilities of HPLC. Automation will streamline procedures, increasing speed and consistency, while improvements in stationary phase chemistry will boost separation performance. This review will help the readers to understand the role and significance of this pivotal analytical technique in biologicals and pharmaceutical quality control.

Keywords: Chromatography, HPLC, Applications, Advancements, RRLC, UPLC, UFLC, Nano Liquid Chromatography.

[This article belongs to Special Issue under section in Journal of Polymer and Composites (jopc)]

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How to cite this article:
Aayushi Agarwal Bansal, Samixa Patel. Recent Advancements in High-Performance Liquid Chromatography: A Comparative Approach. Journal of Polymer and Composites. 2024; 13(01):40-48.
How to cite this URL:
Aayushi Agarwal Bansal, Samixa Patel. Recent Advancements in High-Performance Liquid Chromatography: A Comparative Approach. Journal of Polymer and Composites. 2024; 13(01):40-48. Available from: https://journals.stmjournals.com/jopc/article=2024/view=187832


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References

  1. Ettre LS, Tswett MS. The invention of chromatography. LC-GC. 2003;21:458-467.
  2. Yandamuri N. Comparative Study of New Trends in HPLC: A Review. Int J Pharm Sci Rev Res. 2013;23(2):52-57.
  3. Yoshida T, Ronald EM, Kumagai H. High-Speed Analyses using Rapid Resolution Liquid Chromatography on ZORBAX column packed 1.8 μm Particles. Chromatography. 2007;28:81-87.
  4. Van Deemter JJ, Zuiderweg EJ, Klinkenberg A. Longitudinal diffusion and resistance to mass transfer as causes of nonideality in chromatography. Chem Eng Sci. 1956;5:271-289.
  5. Elisha RP. Comparative study of new trends in HPLC. IJPRBS. 2013;2(3):167-172.
  6. Ian A, Darwin A, Eddie G, Christensen J, Ryan M. Rapid Resolution Liquid Chromatography with Charged Aerosol Detection. Magellan Biosciences. 567-576.
  7. Maa YC, Wanga XQ, Houa FF, Luoa MCA, Jin P, Lua SXI. Rapid resolution liquid chromatography (RRLC) analysis and studies on the stability of Shuang-Huang-Lian preparations. J Pharm Biomed Anal. 2011;54:265-272.
  8. Haripriya P. A review on rapid resolution liquid chromatography of Agilent1200 RRLC series. WJPPS. 2013;4(07):1581-1595.
  9. Lodha L, Sharma N, Viswas A, Khinchi MP. A review on chromatography techniques. Asian J Pharm Res Devel. 2017;5(2):1-8.
  • Swartz ME, Murphy BJ. Ultra performance liquid chromatography: Tomorrow’s HPLC technology today, novel phases for HPLC. LPI. 2004 Jun;21:147-156.
  1. Meenakshi D, Meenaxi M. A Review on Ultra Performance Liquid Chromatography. Int J Drug Dev Res. 2013;5:29-34.
  2. Kumar A, Gautam S, Anroop N, Rishbha S. Uplc: A Preeminent Technique in Pharmaceutical Analysis. Acta Pol Pharm. 2012;69:371-380.
  3. Swartz ME, Murphy. Formulation Quality. 2004;6-40.
  4. Reddy SK, et al. Ultra Performance Liquid Chromatography: An Introduction and Review. 2012;2(1):24-31.
  5. Nguyen DT, Guillarme D, Rudaz S, Veuthey JL. Fast analysis in liquid chromatography using small particle size and high pressure. J Sep Sci. 2006;29(12):1836-1848.
  6. Jerkovitch AD, Mellors JS, Jorgenson JW. LC-GC. 2003;21(7):23-32.
  7. Goodwin L, White SA, Spooner N. Evaluation of ultra-performance liquid chromatography in the bioanalysis of small molecule drug candidates in plasma. J Chromatogr Sci. 2007;45(6):298-304.
  8. Swartz M. LC-GC. 2005;23(1):46-53.
  9. Broske AD, et al. Agilent Technologies application note. 2004:5988-9251EN.
  10. Ultra-fast liquid chromatography [Internet]. Available from: http//Schimadzu.co.UK.
  11. Analysis of artificial colorants by UFLC Mass.
  12. Malviya R, Bansal V. High performance liquid chromatography: A short review. J Global Pharm Technol. 2010;2(5):22-26.
  13. Bansal AA. Determination of Etoricoxib in bulk formulations by Rp-Hplc Method. World J Pharmaceutical Sciences. 2022; 11(15): 1128-1136.
  14. Koli RS, Patel AS, Chaudhari KN, Patil KR. A review on HPLC and its new trends. Asian J Pharm Anal. 2018;8(4):233-236.
  15. Sun N, et al. An ultra-fast LC method for the determination of iodiconazole in micro-dialysis samples and its application in the calibration of laboratory-made linear probes. J Pharm Biomed Anal. 2010;51:248-251.
  16. Wang XP, Zeng K, Li GF, Chen LY, Ma AD, Wang L. Quantitative determination of podophyllotoxin in dermal and blood micro-dialysis samples of rats by liquid chromatography-tandem mass spectrometry. J Southern Med Univ. 2010;30:1256-1267.
  17. Qiao F, Yan H. Simultaneous analysis of fluoroquinolones and xanthine derivatives in serum by molecularly imprinted matrix solid-phase dispersion coupled with liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2011;879:3551-5.
  18. Borge JH, Aturki Z. Recent applications in nano-liquid chromatography. J Sep Sci. 2007;30:1589-1610.
  19. Qing H. Integrated nano liquid chromatography system on a chip [PhD thesis]. Pasadena (CA): California Institute of Technology; 2005 Jun 21.
  20. Nano Liquid Chromatography [Internet]. [cited 2007 Jun 18].
  21. Available from: http://www.justchromatography.com/nanohplc/columns-in-nano-lc.
  22. Srinivas P, Muralikrishna P, Sadanandam M. Int.J.Pharm.Sci. 2010;2:728-737.
  23. Orazio GD, Rocchi S, Fanali S. Nano-liquid chromatography coupled with mass spectrometry: separation of sulfonamides employing non-porous core-shell particles. J Chromatogr A. 2012;1255:277-285.
  24. Tao XS, Yuan H, Zhou Y. Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and peptide separation for proteome analysis. J Sep Sci. 2012;35:1764-1770.
  25. Hua S, Lebrilla C, An HJ. Application of nano-LC-based glycomics towards biomarker discovery. Bioanalysis. 2011;3:2573-2585.
  26. Kirsch S, Bindila L. Nano-LC and HPLC-chip-ESI-MS: an emerging technique for glycobioanalysis. Bioanalysis. 2009;1:1307-1327.
  27. Saito Y, Jinno K, Greibrokk T. Capillary columns in liquid chromatography: Between conventional columns and microchips. J Sep Sci. 2004;27:1379-1390.
Special Issue Open Access Original Research
Volume 13
Special Issue 01
Received 02/03/2024
Accepted 09/09/2024
Published 05/12/2024
Publication Time 278 Days
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