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- Student,Al_Iraqia University,Baghdad,Iraq
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n In this study, a rapid, easy, eco-friendly and pollution-free-polution method to synthesize Ag
nanoparticles (NPs) was developed. Laser ablation technique was used to bulk target silver in harsh
environment which was simulated body fluid due to salts and ions content cause Ag NPs to aggregate
via the interactions between NPs and components of the ionic solution. Ovalbumin was added to
achieve long-term stability of colloidal Ag NPs solution. This protein has been used to decrease ionic
strength and avoid nanoparticle agglomeration in biofluids by attaching to the Ag NPs surfaces in the
mixture, thereby protecting them from the NaCl and preventing aggregation. The color change on the
sample was observed and we examined it with a transmission electron microscope to see its size and
shape, as well as the zeta potential to know its charge, in addition to measuring it in Fourier
transform infrared (FTIR) and UV-vis spectrophotometer over a month to prove the stability of the
sample. The Ag NPs solution and their dilutions were applied on cultured media of Candida albicans
which was isolated from human patients with candida infection.n
Keywords: Silver nanoparticles (Ag NPs), pulse laser ablation (PLAL), UV-vis, FTIR, z-potential, TEM, Candida albicans
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|Received||December 8, 2022|
|Accepted||January 31, 2023|
|Published||April 18, 2023|
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