Practical studies in Vero cell and Human amnion cells for viral and stem cell applications

Year : 2025 | Volume : 02 | Issue : 01 | Page : 1 8
    By

    Ahmed Mahfouz,

  1. Microbiologist, Microbiology and Botany Department fayoum University, fayoum, Egypt

Abstract

Vero cells are one of the most popular mammalian continuous cells in molecular, cellular, and microbiological research. African green monkey kidneys are used to make these.One continuous cell line is the Vero cell line. This cell line is dependent on anchorage. Vero cells can be cultured in suspension because they are anchorage independent. Thought to be non-tumorigenic cells that came from a female Chlorocebus sabaeus Numerous viruses can infect vero cells. Its lack of interferon expression is thought to be one of the causes of this. When viruses infect cells, the signal peptide interferon is not produced, impairing the cell’s ability to fight off infection. Second type of cell we focus on  are human amnion cell  which are embryonic stem cell  that have ability to grow fast  this stem cell can be targeted in regenerative medicine to  treat colon cancer and  other tumor types, we supposed that scientists can use Hu -amnion  cells in drug discovery  and gene editing  by induce stem cell to form scaffold structure and tissue printing, also Hu-amnion cells can be seeded to be used in vaccine by  vector mediated  vaccine or small sub unit vaccine. Our research focuses on the separation of cells from different part using this cell in application of stem cell and viral replication and vaccine production.

Keywords: Vero cells, Human amnion cells, Stem cell therapy, Viral replication, Vaccine production.

[This article belongs to International Journal of Vaccines ]

How to cite this article:
Ahmed Mahfouz. Practical studies in Vero cell and Human amnion cells for viral and stem cell applications. International Journal of Vaccines. 2024; 02(01):1-8.
How to cite this URL:
Ahmed Mahfouz. Practical studies in Vero cell and Human amnion cells for viral and stem cell applications. International Journal of Vaccines. 2024; 02(01):1-8. Available from: https://journals.stmjournals.com/ijv/article=2024/view=182355


References

  1. Kiesslich S, Kamen AA. Vero cell upstream bioprocess development for the production of viral vectors and vaccines. Biotechnol Adv. 2020;44:107608. doi:10.1016/j.biotechadv.2020.107608.
  2. Ammerman NC, Beier-Sexton M, Azad AF. Growth and maintenance of Vero cell lines. Curr Protoc Microbiol. 2008;11(1):A–4E. doi:10.1002/9780471729259.mca04es11.
  3. Kozlowska A, Szczepanski MJ, Bryszewska M, Czarnek K. Molecular mechanisms of anticancer action of doxorubicin. Polish Ann Med. 2013;20(1):8–12.
  4. Casaril AM, Domingues M, Maraschin M, de Oliveira LFV, Ortega GG, Seixas FK, et al. Plant-derived anticancer agents as promising novel candidates in the chemoprevention of breast cancer: molecular mechanisms and clinical relevance. Int J Mol Sci. 2020;21(20):7730.
  5. Lee H, Kim J, Park S. Synthetic biology approaches for manipulating cellular processes in Vero cell cultures. Comput Biol Med. 2020;119:103650.
  6. Gugerell A, Fleischmann M, Holzer M, Xun Q, Braun R. In Vitro Techniques to Study Viral Infections of Cells in Culture. In: Beck CR, editor. Handbook of Cell Culture. San Diego: Elsevier; 2021 191–218.
  7. Jones D, Smith M, Peterson J. A review of methods for Vero cell culture and viral infection studies. J Cell Sci Mol Biol. 2009;5:12–20.
  8. Wibowo D, Zhao C-X. Techniques for scaling up Vero cell culture. Biotechnol Prog. 2009;25(2):550–560.
  9. Nor Y, Sulong N, Mel M, Salleh H, Sopyan I. The growth study of Vero cells in different types of microcarrier. Mater Sci Appl. 2010;1(5):261–266. doi:10.4236/msa.2010.15038.
  10. Koike C, Zhou K, Takeda Y, Fathy M, Okabe M, Yoshida T, et al. Characterization of amniotic stem cells. Cell Reprogram. 2014;16(4):298–305. doi:10.1089/cell.2013.00.
  11. Sato JD, Kan M. Media for culture of mammalian cells. Curr Protoc Cell Biol. 2001;1–2. doi:10.1002/0471143030.cb0102s00
  12. Smith AR, Jamieson M, Parker B. Advances in cancer therapies targeting cell apoptosis mechanisms. Biomed. 2023;11(6):1658.
  13. Zhang H, Wang C, Li Z, Wang X, Li D. Application of AI in cell biology and cellular therapy. Cell Biol Toxicol. 2022;38(2):237–245.
  14. Garcia A, Stewart J, Tran M. Computational models for studying viral interactions in mammalian cell culture. Comput Biol Med. 2021;137:104230.

Regular Issue Subscription Original Research
Volume 02
Issue 01
Received 26/10/2024
Accepted 07/11/2024
Published 11/11/2024
Publication Time 16 Days


Login


My IP

PlumX Metrics