Rajni Tanwar,
Ramesh Kumar,
Amandeep Mor,
Dinesh Kumar,
- Associate Professor, School of Pharmacy, Desh Bhagat University, Mandi Gobindgarh,, Punjab, India
- Research Scholar, GJU&ST, Hisar, Haryana, India
- Research Scholar, Om Sterling Global University, Hisar, Haryana, India
- Associate Professor, School of Pharmacy, Desh Bhagat University, Mandi Gobindgarh, Punjab, India
Abstract
In terms of public health, tuberculosis continues to have a significant impact on people’s lives. The process of determining the underlying cause of a sickness or condition in a patient is known as clinical diagnosis. It entails the use of numerous diagnostic methods, including physical examinations, laboratory testing, and imaging procedures for medical purposes. Smear microscopy, which are frequently utilized, have poor sensitivity and lengthy turnaround times, respectively. An innovative, quick, cartridge-based molecular test called the GeneXpert (GX) assay has a well-established use for the quick identification of pulmonary and extrapulmonary Mycobacterium tuberculosis (MTB) infections. This device delivers a report indicating whether the sample tested positive or negative for TB after around two hours. The report offers details on the quantity of TB bacteria found in the sample, which can help determine treatment choices. HIV, hepatitis C, and gonorrhea are just a few of the infectious disorders that the GeneXpert system can identify. It is also used to detect the SARS-COV-2 virus-related illness known as COVID-19.
Keywords: GeneXpert, mycobacterium tuberculosis, diagnosis, infection, rapid testing technologies
[This article belongs to International Journal of Pathogens ]
Rajni Tanwar, Ramesh Kumar, Amandeep Mor, Dinesh Kumar. Cartridge-Based Nucleic Acid Amplification for Rapid Tuberculosis Diagnosis. International Journal of Pathogens. 2024; 01(01):43-48.
Rajni Tanwar, Ramesh Kumar, Amandeep Mor, Dinesh Kumar. Cartridge-Based Nucleic Acid Amplification for Rapid Tuberculosis Diagnosis. International Journal of Pathogens. 2024; 01(01):43-48. Available from: https://journals.stmjournals.com/ijpg/article=2024/view=146949
References
- Morales-López SE, Yepes JA, Anzola I, Aponte H, Llerena-Polo CR. Paradoxical results of two automated real-time PCR assays in the diagnosis of pleural tuberculosis. Int J Infect Dis. 2017;54:36-8.
- Macedo R, Antunes AF, Villar M, Portugal I. Multidrug and extensively drug-resistant tuberculosis in Lisbon and Vale do Tejo, Portugal, from 2008 to 2010. Int J Mycobacteriol. 2012;1(3):131-6.
- Garg RK, Somvanshi DS. Spinal tuberculosis: a review. J Spinal Cord Med. 2011;34(5):440-54. doi:10.1179/2045772311Y.0000000023.
- Sharma SK, Ryan H, Khaparde S, Sachdeva KS, Singh AD, Mohan A, et al. Index-TB guidelines: guidelines on extrapulmonary tuberculosis for India. Indian J Med Res. 2017;145(4):448-63.
- Sharma SK, Mohan A. Extrapulmonary tuberculosis. Indian J Med Res. 2004;120:316-53.
- Lawn SD, Zumla AI. Diagnosis of extrapulmonary tuberculosis using the Xpert® MTB/RIF assay. Expert Rev Anti Infect Ther. 2012;10(6):631-5.
- Arockiaraj J, Michael JS, Amritanand R, David KS, Krishnan V. The role of Xpert MTB/RIF assay in the diagnosis of tubercular spondylodiscitis. Eur Spine J. 2017;26:3162-9.
- Solanki AM, Basu S, Biswas A, Roy S, Banta A. Sensitivity and specificity of gene Xpert in the diagnosis of spinal tuberculosis: a prospective controlled clinical study. Glob Spine J. 2020;10(5):553-8.
- Reither K, Manyama C, Clowes P, Rachow A, Mapamba D, Steiner A, et al. Xpert MTB/RIF assay for diagnosis of pulmonary tuberculosis in children: a prospective, multi-centre evaluation. J Infect. 2015;70(4):392-9.
- Steingart KR, Sohn H, Schiller I, Kloda LA, Boehme CC, Pai M, et al. Xpert® MTB/RIF assay for pulmonary tuberculosis and rifampicin resistance in adults. Cochrane Database Syst Rev. 2013;(1):CD009593.
- Denkinger CM, Schumacher SG, Boehme CC, Dendukuri N, Pai M, Steingart KR. Xpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis: a systematic review and meta-analysis. Eur Respir J. 2014;44(2):435-46.
- Steingart KR, Ng V, Henry M, Hopewell PC, Ramsay A, Cunningham J, et al. Sputum processing methods to improve the sensitivity of smear microscopy for tuberculosis: a systematic review. Lancet Infect Dis. 2006;6(10):664-74.
- Moore DF, Guzman JA, Mikhail LT. Reduction in turnaround time for laboratory diagnosis of pulmonary tuberculosis by routine use of a nucleic acid amplification test. Diagn Microbiol Infect Dis. 2005;52(3):247-54.

International Journal of Pathogens
| Volume | 01 |
| Issue | 01 |
| Received | 11/05/2024 |
| Accepted | 15/05/2024 |
| Published | 20/05/2024 |
| Publication Time | 9 Days |
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