Evaluation of Antioxidant and Cytotoxic activities of Mycelia Biomass and Culture broth of three Edible Mushrooms, Morchella conica, Hericium erinaceus and Pleurotus florida Grown in Submerged Culture

Year : 2024 | Volume : 01 | Issue : 01 | Page : 37-50
    By

    Sneha Das,

  • Frangleena P S,

  • Kainoor Krishnankutty Janardhanan,

  1. Research Associate, Amala Cancer Research Centre Society, Amala Nagar, Thrissur, Kerala, India
  2. Project Fellow, Department of Biomedical Sciences, Vellore Institute of Technology, Vellore, Chennai, India
  3. Professor, Amala Cancer Research Centre Society, Amala Nagar, Thrissur, Kerala, India

Abstract

Mycelia biomass of three edible mushrooms  namely, Morchella conica, Pleurotus florida and Hericium erinaceus  were grown as submerged culture in potato dextrose broth supplemented with yeast extract, potassium dihydrogen orthophosphate and magnesium sulphate. The mycelia biomass was extracted with 70% ethanol and culture broth  with ethyl acetate. The solvents were evaporated to dryness at low temperature using a rotary vacuum evaporator and the residues thus obtained were used for the studies.. The extracts of  mycelia biomass and culture broth were evaluated for antioxidant, cytotoxic  and antiproliferative activities. Antioxidant activity of extracts was assayed by DPPH (2,2-dyphenyl- 1- picrylhydozyl ) radical scavenging assay, FRAP (Ferric ion reducing antioxidant power), superoxide radical scavenging  and lipid peroxidation inhibiting assays. Cytotoxicity of mycelia and culture broth extracts were assayed by MTT ( 3-(4,5-Dimethylthiazol-2-yl)-2-5 diphenyltetrazolium bromide ) assay using HepG2 liver cancer cell line and antiproliferative activity by using DLA (Dalton’s Lymphoma Ascites ) cell line.  Among three mushrooms examined, ethanol extract of mycelia of M.conica and ethyl acetate extract of culture broth of  H. erinaceus possessed highest antioxidant activity.The extracts of M. conica and H. erinaceus had a significant cytotoxic effect against DLA cells than P. florida.. Ethyl acetate extract of culture broth of H. erinaceus showed the highest cytotoxic activity against HepG2 liver cancer cell lines.  The experimental results suggest the possible use of the mycelia and culture broth extracts of these mushrooms as supplements in cancer treatment. The findings also reveal the mycelia and culture broth of medicinal mushrooms have similar therapeutic potential as the fruiting bodies.

Keywords: Morchella conica, Pleurotus florida, Hericium erinaceus, mycelia biomass, culture broth.

[This article belongs to International Journal of Fungi ]

How to cite this article:
Sneha Das, Frangleena P S, Kainoor Krishnankutty Janardhanan. Evaluation of Antioxidant and Cytotoxic activities of Mycelia Biomass and Culture broth of three Edible Mushrooms, Morchella conica, Hericium erinaceus and Pleurotus florida Grown in Submerged Culture. International Journal of Fungi. 2024; 01(01):37-50.
How to cite this URL:
Sneha Das, Frangleena P S, Kainoor Krishnankutty Janardhanan. Evaluation of Antioxidant and Cytotoxic activities of Mycelia Biomass and Culture broth of three Edible Mushrooms, Morchella conica, Hericium erinaceus and Pleurotus florida Grown in Submerged Culture. International Journal of Fungi. 2024; 01(01):37-50. Available from: https://journals.stmjournals.com/ijf/article=2024/view=157744



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Regular Issue Subscription Original Research
Volume 01
Issue 01
Received 15/06/2024
Accepted 22/07/2024
Published 23/07/2024
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