Regional Variation in Amino Acid Composition, Antioxidant and Antimicrobial Activity of Tasar Sericin from North and South Bihar

Year : 2026 | Volume : 16 | 02 | Page :
    By

    Md Tahfizur Rahman,

  • Rahul Chaubey,

  • Kumar Manish,

  • Shagufta Nigar,

  • Tushar Kumar,

  1. Assistant Professor, P.G. Department of Zoology, Millat College, Darbhanga, Bihar, India
  2. Project Research Scientist-II, Kala-azar Medical Research center, Rambagh, Muzaffarpur, Bihar, India
  3. Assistant Professor, University P.G. Department of Zoology, L.N Mithila University, Darbhanga, ,
  4. Assistant Professor, P.G. Department of Zoology, Millat College, Darbhanga, Bihar, India
  5. Research Scholar, P.G. Department of Botany, L.N Mithila University Darbhanga, Bihar, India

Abstract

This study investigates geographical differences in biochemical and functional characteristics of sericin obtained from Antheraea mylitta cocoons sourced from two regions of Bihar, India: Bhagalpur (North) and Banka (South). Sericin was isolated via an alkali-assisted autoclaving process. Amino acid profiles were determined using HPLC. Free radical scavenging capacity was measured through DPPH, ABTS, and hydrogen peroxide assays. Antibacterial and antifungal effects were tested against Staphylococcus aureus, Escherichia coli, and Candida albicans using disk diffusion and MIC methods. Marked regional differences (p<0.05) were observed. South Bihar sericin contained higher serine (21.34% vs 18.67%) and aspartic acid (16.78% vs 14.92%). Antioxidant activity was stronger in South samples, with DPPH IC₅₀ of 82.34±3.21 μg/mL compared to 124.67±4.56 μg/mL for North samples. Antimicrobial testing revealed larger inhibition zones for South sericin against S. aureus (14.2±0.8 mm) and E. coli (12.8±0.6 mm) at 100 μg/mL. Positive correlations existed between serine content and antioxidant activity (r=0.89, p<0.01) and between phenolics and antimicrobial effects. These findings confirm that tasar sericin bioactivity varies significantly by region, with South Bihar material showing superior properties, likely due to differences in host flora, soil chemistry, and ecorace genetics.

Keywords: Tasar sericin, Antheraea mylitta, regional variation, antioxidant, antimicrobial, amino acid composition

How to cite this article:
Md Tahfizur Rahman, Rahul Chaubey, Kumar Manish, Shagufta Nigar, Tushar Kumar. Regional Variation in Amino Acid Composition, Antioxidant and Antimicrobial Activity of Tasar Sericin from North and South Bihar. Research and Reviews : A Journal of Life Sciences. 2026; 16(02):-.
How to cite this URL:
Md Tahfizur Rahman, Rahul Chaubey, Kumar Manish, Shagufta Nigar, Tushar Kumar. Regional Variation in Amino Acid Composition, Antioxidant and Antimicrobial Activity of Tasar Sericin from North and South Bihar. Research and Reviews : A Journal of Life Sciences. 2026; 16(02):-. Available from: https://journals.stmjournals.com/rrjols/article=2026/view=244047


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Ahead of Print Subscription Original Research
Volume 16
02
Received 08/05/2026
Accepted 14/05/2026
Published 15/05/2026
Publication Time 7 Days
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