Monitoring The Hydrocarbon Utilizing Bacteria in Crude Oil Remediation Using Bio-Stimulant of Mint Leaf (Mentha Piperita)

Year : 2026 | Volume : 16 | Issue : 01 | Page : 34 39
    By

    Joy Chukwuemeka Peter Ukpaka,

  • Abraham Peter Ukpaka,

  • Victor Chukwuemeka Ukpaka,

  • Ukpaka Chukwuemeka Peter,

  1. Research Student, Department of Pharmacy, MSB Medical School Berlin GmbH – Hochschule für Gesundheit und Medizin, Berlin, Germany
  2. Research Student, College of Engineering, Computer Studies and Architecture, Department of Computer Engineering, Lyceum of the Philippines University, Cavite, Phillipines
  3. Research Student, College of Engineering, Computer Studies and Architecture, Department of Industrial Engineering, Lyceum of the Philippines University, Cavite, Phillipines
  4. Professor, Department of Chemical/Petrochemical Engineering, Rivers State University Port Harcourt, River state, Nigeria

Abstract

Conceptual monitoring of hydrocarbon utilization bacteria on crude oil remediation using bio-stimulant of mint leaf (Mentha piperita) was investigated. The bio-stimulant nutrient was determined by considering the available nitrogen, phosphorus, potassium, and hydrocarbon utilizing bacteria presence in both room and sun-dried samples of the remediant used. The mint leaf (Mentha piperita) was processed to obtain the powder form (nano particle size) of the bio-stimulant and the microbes isolated and identified from the crude oil and loamy soil was classified as hydrocarbon utilizing bacteria and fungi. The microbial performance was monitored by setting-up different bioreactors labeled A1, A2, B1, B2, and C representing the application of room-dried bio-stimulant application (A1 and A2), sun-dried for B1 and B2 of 50g and 100g dosage, whereas C represent control sample. The microbial activities were monitored for 35 days and the concentration of the hydrocarbon utilizing bacteria determined. The microbial growth was high on bioreactors that contains the bio-stimulant of room-dried sample that those that contain sun-dried sample and the variation was integrated to the characteristics and physicochemical properties of the NPK nutrient at end product obtained after processing the mint leaf (Mentha piperita) of the powder form. The conceptual behaviors of the HUB were experienced from the 7 days to 35 days in terms of phase phenomena as demonstrated and this revealed no lag phase, acceleration phase, progressive phase, stationing phase and death or decline phase. The increase in the HUB growth was experienced at stages of acceleration and progressive phase. The increase in the HUB growth was experienced at stages of acceleration and progress phase. The rapid growth by the HUB enhanced the effectiveness in the crude oil remediation and the microbial activities were not inhibited by any environmental factors. Finally, the research has revealed the effectiveness of the available nutrient of the mint leaf (Mentha piperita) in enhancing remediation of polluted soil environment.

Keywords: Bio-stimulant, crude oil, HUB, mentha piperita, mint leaf, monitoring

[This article belongs to Journal of Materials & Metallurgical Engineering ]

How to cite this article:
Joy Chukwuemeka Peter Ukpaka, Abraham Peter Ukpaka, Victor Chukwuemeka Ukpaka, Ukpaka Chukwuemeka Peter. Monitoring The Hydrocarbon Utilizing Bacteria in Crude Oil Remediation Using Bio-Stimulant of Mint Leaf (Mentha Piperita). Journal of Materials & Metallurgical Engineering. 2026; 16(01):34-39.
How to cite this URL:
Joy Chukwuemeka Peter Ukpaka, Abraham Peter Ukpaka, Victor Chukwuemeka Ukpaka, Ukpaka Chukwuemeka Peter. Monitoring The Hydrocarbon Utilizing Bacteria in Crude Oil Remediation Using Bio-Stimulant of Mint Leaf (Mentha Piperita). Journal of Materials & Metallurgical Engineering. 2026; 16(01):34-39. Available from: https://journals.stmjournals.com/jomme/article=2026/view=241281


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Regular Issue Subscription Original Research
Volume 16
Issue 01
Received 06/01/2026
Accepted 28/01/2026
Published 21/02/2026
Publication Time 46 Days
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