Farah Jaber Kazem Satoot,
Elham Jawad Kadhum,
- Assistant Lecturer, Department of Biology, The General Directorate of Education, Najaf, , Iraq
- Assistant Professor, Department of Biology, College of Education for Girls, University of Kufa, , Iraq
Abstract
Recently, a noticeable increase in the prevalence of occult Hepatitis B virus (HBV) and Hepatitis C virus (HCV) infections has been observed among clinical cases such as patients undergoing hemodialysis, blood transfusion, liver diseases, and thalassemia worldwide. To limit and control this spread, the present study was conducted to investigate and detect the molecular presence of HCV and occult HBV using the Nested PCR technique, as well as to observe the effect of demographic and social factors on the infection rate. A total of 150 clinical samples were collected from both male and female patients of different age groups and various residential areas. Samples were obtained from the Blood Center, Central Public Health Laboratory, Al-Sadr Medical City, Kidney Center, Al-Zahraa Teaching Hospital, the National Hospital, and the Gastroenterology and Liver Hospital in Al-Najaf Al-Ashraf Governorate. The global prevalence of HCV and occult HBV among hemodialysis, blood transfusion, liver, and thalassemia patients indicates a strong association with healthcare centers. Therefore, preventive and monitoring procedures are highly essential to reduce the transmission of infection. Patients visiting these centers for treatment should be guided on personal hygiene, vaccination, and appropriate medical follow-up, in collaboration with the Training Department of the Directorate of Health and the Patient Safety Division. In particular, therapeutic hemodialysis procedures should be carefully organized in a sequence that ensures patient safety without affecting the treatment schedules in hemodialysis departments.
Keywords: PCR, HBV, HCV, liver Cancer , hepatitis
Farah Jaber Kazem Satoot, Elham Jawad Kadhum. Genetic Detection of Hepatitis C -Virus and Occult Hepatitis B in Patients from Al-Najaf Al-Ashraf Governorate, Iraq.. Research and Reviews: A Journal of Microbiology and Virology. 2025; 16(01):-.
Farah Jaber Kazem Satoot, Elham Jawad Kadhum. Genetic Detection of Hepatitis C -Virus and Occult Hepatitis B in Patients from Al-Najaf Al-Ashraf Governorate, Iraq.. Research and Reviews: A Journal of Microbiology and Virology. 2025; 16(01):-. Available from: https://journals.stmjournals.com/rrjomv/article=2025/view=236227
References
- Al-Obaidi, M. A. (2019). Prevalence of hepatitis B and C viruses among hemodialysis patients in Iraq. Journal of Medical Virology.
- Hussein, A. M. (2020). Seroprevalence of HBV and HCV infections in thalassemia patients. Iraqi Journal of Science.
- Zanetti, A. R., et al. (2018). Occult hepatitis B infection: Molecular and clinical significance. Hepatology Research.
- Mohammed, S. H., et al. (2021). Detection of HBV genotypes using nested PCR among blood donors. International Journal of Microbiology.
- WHO (2022). Global Hepatitis Report. World Health Organization.
- WHO (World Hepatitis Summit) (2022) Achieving the elimination of Viral hepatitis within evolving health systems.
- Eng, F. J.; El-Shamy, A.; Doyle, E. H.; Klepper, A.; Muerhoff, A. S. and Branch, A-D. (2018) Newly discovered hepatitis C virus minicourse circulate in human blood – Hepatology communication. 2:21-8
- Even, D. M.; Stewart, P. W.; Amador, J.; Serper, M.; Lok, A. S.; Sterling, R. K. and et al. (2018). Acomprehensive assessment of patient reported symptom burden, medical comorbidities, and Functional well being In patients initiating direct adding antiviral. therapy Por chronic hepatitis C: results from a large Us multi-center observational Study plos one 13:e 0196908.
- Maryam, T.; Manouchehr, M.; Ali, Ti Maryam, D.; Hamid, EM; Mahdi, Pi Roya p; and Chiman, K. (2018) -Epidemiology and Molecular Detection of HAVI HBV, and HCV in patients with Acute Hepatitis = Symptoms in Ahvaz Journal of Microbiology 11(5): e 63317
- Yusra Ali Kareem Ali, Elham jawad Kadhum. (2025). Molecular Detection of HPV High-Risk Genotypes 16 and 18 in Women with Breast Tumors in Najaf Governorate., Al-Harf , No.24 ., Vol. 1, March 2025
- WHO (2015) National Centers for disease control and prevention. Bary Schat, C. from web site www.pkids.orgoz oqglobalhbu pdf.
- Skhan, S., Attaullah, S. Khan, S.N., Sirag,S & Khan, J. (2011). Rising burden of Hepatitis C virus in hemodialysis Patients. Virology Journal, 8(1), 438
- Amir, S.; Reza A; Ali, AP; Reza, A and Ali, R.M (2014) Serological Markers in Hepatitis B Virus patients Cihan University- Erbil Scientific Journal 4(1).
- Aqeel Jdah Fenjan. (2024). Relationship between Hepatitis B virus Infection and Renal Failure in Al-Najaf Province (A cross Sectional Study)., Al-Harf Journal., No. 23., Vol. 4, December 2024
- Wiktor, S-2 and Hut in, y. J (2016) – The global burden of viral hepatitis better estimates to guide hepatitis elimination efforts, Lancet 388: 1030-1031.
- WHO (2022) prevention and control of viral Hepatitis Infection Framework for Global Action Available on line – https://www.who.int/hepatitis/Publications/Framwork/en/accessedon // Augusts.
- Rusul Imad Abdulkadhim ,Zainab Hussein Mohammed. (2024). Iron Overload and Elevated Beta 2 Micro globulin Levels in Patients with Beta Thalassemia Major., Al-Harf Journal., Vol. 22., Issue. August, 2024.
- Rahman; 5.(2015). Brief Guidelines for Methods and Statistics in Medical Research. 1st edition Springer Singapore Herdelberg New York Dordrecht London.
- Liu, K.; Luckenbaugh, L., Ning, X; Xi, J. and Hus J. (2018). Multiple roles of care protein linker in hepatitis B virus replication plos pathogens-luze 1007025
- M.H, Hassanain Jameel Ali, Zahraa Hussein Ali. (2024). Review: The Effects of Ciprofloxacin on Histological Structure of Organs especially Teste’s in Male Albino Rabbits., Al-Harf Journal, No. 23., Vol. 4 ,December 2024
- Liu, J. Wu, H. and chen, H. (2018) Immune response to hepatitis B vaccine in patients with chronic hepatits Coinfection: A Systematic review and metanalysis. – Hepatology Research -48=119-126.
- Kramuis, A. (2014) Genotype and genetic Variability of hepatitis B virus. Intervirology-57-141-150.
- Kramuis, A.; Arbuthnot P., Swaby, L.A.J Konadu, C. (2014) Hepatitis Buirus prevalence, natural history, and treatment in Africans in Africa and the Unitedt States Cur Hepatitis B Rep, 2, 143 49.

Research and Reviews: A Journal of Microbiology and Virology
| Volume | 16 |
| 01 | |
| Received | 21/11/2025 |
| Accepted | 18/12/2025 |
| Published | 20/12/2025 |
| Publication Time | 29 Days |
Login
PlumX Metrics