Molecular Characterization Phylogenetic Analysis from E.coli Isolated from Human and Cows Raw Milk

Year : 2025 | Volume : 15 | 03 | Page :
    By

    Hasanain Jameel Ali,

  • Zahraa Hussein Abdulateef Ali,

  1. Lecturer, Department of Health Management Techniques, Najaf Technical Institute, Al- Furat Al-Awsat Technical University, , Iraq
  2. Lecturer, Department of Preventive and Orthodontic Dentistry, Najaf Technical Institute, Al-Frurat Al-Awsat Technical University, , Iraq

Abstract

E. coli is a significant pathogen that contributes to the genesis of both human and animal diseases. 20 different E. coli isolates were found in milk, stool, and utensil samples. Depending on whether each E. coli strain has the gene, the chuA, and the DNA segment TspE4.C2 has joined. the intestinal groups A and B1 as well as the extraintestinal groups B2 and D. The findings of the PCR-based analysis revealed that the B2 3 subgroup contained 6 feces samples, 4 milk samples and 2 utensil samples, where these groups are separated into six subgroups (A0, A1, B22, B23, D1, D2). While one stool sample, three milk samples, and one utensil sample were D2 subgroup members, two stool samples and one milk sample were A1 group members (intestinal)., the results pointed to However, according to our data, most of the isolates are part of the phylogenetic B2 and D (Extra-intestinal) groups. Because the B2 and D groupings are very common, there could be a potential risk of exposure to pathogenic E. coli., In this investigation, E. coli isolated was divided into phylogenetic groups according to the existence or absence of three genes: chuA , yjaA , and a DNA fragment termed TspE4.C2  .,The B2 3  group 3, in instance, was where a significant proportion of the detected isolates, stated that the presence of the B2 3 subgroup in faeces, milk, and utensils could be an indication of contamination

Keywords: Phylogenetic analysis, E. coli, Cows, Raw milk, Human

How to cite this article:
Hasanain Jameel Ali, Zahraa Hussein Abdulateef Ali. Molecular Characterization Phylogenetic Analysis from E.coli Isolated from Human and Cows Raw Milk. Research and Reviews : A Journal of Life Sciences. 2025; 15(03):-.
How to cite this URL:
Hasanain Jameel Ali, Zahraa Hussein Abdulateef Ali. Molecular Characterization Phylogenetic Analysis from E.coli Isolated from Human and Cows Raw Milk. Research and Reviews : A Journal of Life Sciences. 2025; 15(03):-. Available from: https://journals.stmjournals.com/rrjols/article=2025/view=228144


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Ahead of Print Subscription Original Research
Volume 15
03
Received 29/08/2025
Accepted 22/09/2025
Published 23/09/2025
Publication Time 25 Days
Total Visits: 26


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