Molecular Characterization Phylogenetic Analysis from E.coli Isolated from Human and Cows Raw Milk

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nThis is an unedited manuscript accepted for publication and provided as an Article in Press for early access at the author’s request. The article will undergo copyediting, typesetting, and galley proof review before final publication. Please be aware that errors may be identified during production that could affect the content. All legal disclaimers of the journal apply.n

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Year : 2025 [if 2224 equals=””]27/09/2025 at 12:29 AM[/if 2224] | [if 1553 equals=””] Volume : 15 [else] Volume : 15[/if 1553] | [if 424 equals=”Regular Issue”]Issue : [/if 424][if 424 equals=”Special Issue”]Special Issue[/if 424] [if 424 equals=”Conference”][/if 424] 03 | Page :

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    Hasanain Jameel Ali, Zahraa Hussein Abdulateef Ali,

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  1. Lecturer, Lecturer, Department of Health Management Techniques, Najaf Technical Institute, Al- Furat Al-Awsat Technical University, Department of Preventive and Orthodontic Dentistry, Najaf Technical Institute, Al-Frurat Al-Awsat Technical University, , Iraq, Iraq

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Abstract

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nE. coli is a significant pathogen that contributes to the genesis of both human and animal diseases. 20 different E. coli isolates were found in milk, stool, and utensil samples. Depending on whether each E. coli strain has the gene, the chuA, and the DNA segment TspE4.C2 has joined. the intestinal groups A and B1 as well as the extraintestinal groups B2 and D. The findings of the PCR-based analysis revealed that the B2 3 subgroup contained 6 feces samples, 4 milk samples and 2 utensil samples, where these groups are separated into six subgroups (A0, A1, B22, B23, D1, D2). While one stool sample, three milk samples, and one utensil sample were D2 subgroup members, two stool samples and one milk sample were A1 group members (intestinal)., the results pointed to However, according to our data, most of the isolates are part of the phylogenetic B2 and D (Extra-intestinal) groups. Because the B2 and D groupings are very common, there could be a potential risk of exposure to pathogenic E. coli., In this investigation, E. coli isolated was divided into phylogenetic groups according to the existence or absence of three genes: chuA , yjaA , and a DNA fragment termed TspE4.C2  .,The B2 3  group 3, in instance, was where a significant proportion of the detected isolates, stated that the presence of the B2 3 subgroup in faeces, milk, and utensils could be an indication of contaminationnn

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Keywords: Phylogenetic analysis, E. coli, Cows, Raw milk, Human

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[/if 424][if 424 equals=”Special Issue”][This article belongs to Special Issue under section in Research and Reviews : A Journal of Life Sciences (rrjols)][/if 424][if 424 equals=”Conference”]This article belongs to Conference [/if 424]

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nHasanain Jameel Ali, Zahraa Hussein Abdulateef Ali. [if 2584 equals=”][226 wpautop=0 striphtml=1][else]Molecular Characterization Phylogenetic Analysis from E.coli Isolated from Human and Cows Raw Milk[/if 2584]. Research and Reviews : A Journal of Life Sciences. 23/09/2025; 15(03):-.

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nHasanain Jameel Ali, Zahraa Hussein Abdulateef Ali. [if 2584 equals=”][226 striphtml=1][else]Molecular Characterization Phylogenetic Analysis from E.coli Isolated from Human and Cows Raw Milk[/if 2584]. Research and Reviews : A Journal of Life Sciences. 23/09/2025; 15(03):-. Available from: https://journals.stmjournals.com/rrjols/article=23/09/2025/view=0

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  1. Orskov, F., and I. Orskov. 1992. Escherichia coli serotyping and disease in man and animals. Can. J. Microbiol. 38:699–704
  2. ingen, E., B. Picard, N. Brahimi, S. Mathy, P. Desjardins, J. Elion, and E. Denamur. 1998. Phylogenetic analysis of Escherichia coli strains causing neonatal meningitis suggests horizontal gene transfer from a predominant pool of highly virulent B2 group strains. J. Infect. Dis. 177:642–650.
  3. Boyd, E. F., and D. L. Hartl. 1998. Chromosomal regions specific to pathogenic isolates of Escherichia coli have a phylogenetically clustered distribution. J. Bacteriol. 180:1159–1165.
  4. Johnson JR, Russo TA. Molecular epidemiology of extraintestinal pathogenic (uropathogenic) Escherichia coli. Int J Med Microbiol 2005; 295: 383–404
  5. Moreno, E., A. Andreu, T. Perez, M. Sabate, J. R. Johnson, and G. Prats. 2006. Relationship between Escherichia coli strains causing urinary tract infection in women and the dominant faecal flora of the same hosts. Epidemiol. Infect. 134:1015–1023
  6. Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol 2004;2:123–140.
  7. Rapini LS, Cerqueira MMOP, Carmo LS, Veras JF, Souza MR. Presenc¸a de Staphylococcus spp. produtores de enterotoxinas e da toxina da sı´ndrome do choque to´xico em manipuladores de queijo de cabra. Arq Bras Med Vet Zootec 2005;57:825–829.
  8. R Mohammad Ali J A , S Abdulkereem Habeeb A ,A Fadhil Saeed A ,L Sadiq Mahdi , S Sabah Abd A. (2025). Isolation and Diagnosis of some Bacterial Species from Shoes Sports for Practicing and Non-practicing Female Students for Sports at the College of Education for Girls., Al-Harf Journal., No. 24 ., Vol. 3, August 2025
  9. Guillemot D, Courvalin P. Better control of antibiotic resistance. Clin Infect Dis. 2001;33:542–547. doi:10.1086/322583.
  10. Mesa RJ, Blanc V, Blanch AR, et al. Extended-spectrum b-lactamase producing Enterobacteriaceae in different environments (humans, food, animal farms and sewage). J Antimicrob Chemother. 2006;58:211–215. doi:10.1093/jac/ dkl211.
  11. 1 Omran, R, Al-Fatlawi, I, Inexpensive and reliable rapid manual procedure for extracting genomic DNA from frozen blood samples. Pakistan Journal of Biotechnology.2017, 14(4): 683–687
  12. hmed, N. A.; Mahmood, S. S. and Abbas, A. H.(2019). “A Comparative Study of Some Virulence Factors and Phylogenetic Charactrization of Escherichia.Coli Isolates Causing Urinary Tract Infection and the Commensal Gut.” Iraqi Journal of Agricultural Sciences 50(4): 1193–98.
  13. Rusul N., Jinan M H. (2023). Detection of Virulence Genes of Pathogenic Escherichia coli Isolated from Different Sources., Al-Harf Journal ., Issue August, No. 18, P: 130-138.
  14. Al-Agha, A.G.M.; Al-Khafaji, N.J.M. and Al-Azawi, A.K.S. (2017). Isolation and Identification of Klebsiella pneumoniae using API- 20E analytical system and conventional PCR assay. International Journal of Current Microbiology and Applied Sciences, 6(8): 203- 210.
  15. Musa, M.; Shaker Salman, R. and Mutar, A. (2020). “PhylogeneticBackground, Biofilm and Antibiogram Profile of E.Coli Isolated from Raw Milk Soled in The Local Markets at Al-Nasiriyah City-Iraq, International Journal of Pharmaceutical Research, 11(1):2-8
  16. Alwash, M.S. and Al-Rafyai, H.M. (2019). Antibiotic Resistance Patterns of Diverse Escherichia coli Phylogenetic Groups Isolated from the Al-Hillah River in Babylon Province, Iraq. The Scientific World Journal,(9): 4-9
  17. Merza, N.S. and Jubrael, J.M. (2015). Phylogenetic grouping of uropathogenic Escherichia coli using different molecular typing methods in Kurdistan Region-Iraq. Int. J chem. BioMol. Sci,1 (4):51-55.
  18. Hussain, A. and Saleh, M. (2019). “Determination of Phylogenetic Groups and Antimicrobial Susce-Ptibility Patterns for Escherichia Coli Isolated From Patients With Urinary Tract Infection.” Journal of College of Education for Pure Science 9(1): 71–81.

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Volume 15
[if 424 equals=”Regular Issue”]Issue[/if 424][if 424 equals=”Special Issue”]Special Issue[/if 424] [if 424 equals=”Conference”][/if 424] 03
Received 29/08/2025
Accepted 22/09/2025
Published 23/09/2025
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Publication Time 25 Days

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