Characterization of Plant Growth Promoting Bacillus subtilis (VBKT5) Isolated from Vermicompost

Open Access

Year : 2023 | Volume : | : | Page : –

Sucharita Ghosh

Tilak Nayak

Paltu Kumar Dhal

Tushar Kanti Dangar

Soumendranath Chatterjee

  1. Research Scholar Parasitology and Microbiology Research Laboratory, Department of Zoology, The University of Burdwan West Bengal India
  2. Research Scholar Department of Life Science and Biotechnology, Jadavpur University West Bengal India


Vermicompost is a rich source of nutrients and beneficial soil bacteria which can promote plant growth in various ways. Ten prevalent bacterial strains were isolated from the cow dung vermicompost and assessed for growth promotion of paddy. Bacterial suspensions (3X108 CFU/ml) were individually applied to the pots planted with paddy variety and vegetative growth parameters and yield were recorded. Among the bacterial treatments, better shoot length, root length and yield were recorded for Bacillus subtilis (VBKT5). Average yield in untreated control plants was less than a half of the yield for B. subtilis (VBKT5) treated plant. This strain was Gram positive, rod shaped, endospore forming bacillus and identified as B. subtilis. The results of the present study clearly indicated that B. subtilis isolated from vermicompost significantly boosted up growth and production of paddy plants and the strain may be explored as a plant growth promoting bacteria (PGPB) for rice improvement.

Keywords: Bacillus subtilis, paddy plant, plant growth, PGPR, vermicompost

How to cite this article: Sucharita Ghosh, Tilak Nayak, Paltu Kumar Dhal, Tushar Kanti Dangar, Soumendranath Chatterjee. Characterization of Plant Growth Promoting Bacillus subtilis (VBKT5) Isolated from Vermicompost. Research & Reviews : A Journal of Life Sciences. 2023; ():-.
How to cite this URL: Sucharita Ghosh, Tilak Nayak, Paltu Kumar Dhal, Tushar Kanti Dangar, Soumendranath Chatterjee. Characterization of Plant Growth Promoting Bacillus subtilis (VBKT5) Isolated from Vermicompost. Research & Reviews : A Journal of Life Sciences. 2023; ():-. Available from:

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1. Barman M, Paul S, Choudhury AG, Roy P, Sen J. Biofertilizer as prospective input for sustainable agriculture in India. Int J Curr Microbiol Appl Sci . 2017; 6: 1177–1186p. Available from:
2. Roychowdhury D, Mondal S, Banerjee SK. The effect of biofertilizers and the effect of vermicompost on the cultivation and productivity of maize-a review. Adv Crop Sci Tech. 2017; 5(1): 1–4p. Available from:
3. Hashemimajd K, Kalbasi M, Golchin A, Shariatmadari H. Comparison of vermicompost and composts as potting media for growth of tomatoes. J Plant Nutr. 2004; 27: 1107–23p. Available from:
4. Sinha RK, Agarwal S, Chauhan K, Valani D. The wonders of earthworms and its vermicompost in farm production: Charles Darwin’s ‘friends of farmers’, with potential to replace destructive chemical fertilizers from agriculture. Agric Sci. 2010; 1: 76–94p. Available from:
5. Vennila C, Jayanthi C, Sankaran VM. Vermicompost on crop production—a review. Agric Rev. 2012; 33(3): 265–270p.
6. Munnoli, PM, Silva JAT, Saroj B. Dynamics of the soil-earthworm-plant relationship: A review. Dyn Soil Dyn Plant. 2010; 4: 1–21p.
7. Darzi MT, Hadi MHS. Effects of the application of organic manure and biofertilizer on the fruit yield and yield components in Dill (Anethum graveolens). J Med Plant Res. 2012; 6(17): 3266–3271p. Available from:
8. Matteoli FP, Reis RJA, Passarelli-Araujo LO, Rocha EM, Souza L, Aravind FL, Olivares Venancio TM. Genome sequencing and assessment of plant growth-promoting properties of a Serratia marcescens strain isolated from vermicompost. BMC genom. 2018; 19: 750–755p. Available from:
9. Balasubramanian P, Karthickumar P. Biofertilizers and biopesticides: A holistic approach for sustainable agriculture. In: Mondal P, Dalai AK. Sustainable utilization of natural resources. USA: CRC Press; 2017. 255–284p. Available from:
10. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey’s Manual of Determinative Bacteriology, 9th Edn. Baltimore: The Williams and Wilkins Co.; 1994.
11. Smibert RM, Krieg NR. Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR, editors. Methods of general and molecular bacteriology. Washington DC: American Society for Microbiology; 1994.
12. Logan NS, Vos P, Genus I. Bacillus. In: de Vos P, Garrity GM, Jones D, Krieg NR, Ludwing W, Rainey FA, Schleifer KH, Whitman WB, editors. Bergey’s manual of systematic bacteriology, 3(2). New York: The Firmicutes Springer; 2009.
13. Cappuccino JG, Sherman N. Biochemical activities of microorganisms. In: Microbiology, a laboratory manual; 10th Edn. New Delhi, India: Pearson; 2014.
14. Brown A, Smith H. Benson’s Microbiological Applications, Laboratory Manual in General Microbiology, Short Version. USA: McGraw-Hill Education; 2014.
15. Krumpernam PH. Multiple antibiotic resistance indexing Escherichia coli to identify risk sources of faecal contamination of foods. Appl Environ Microbiol. 1983; 46: 165–170p.
16. Douglas A, Atchison B. Degradation of DNA during the denaturation step of PCR. PCR Methods Appl. 1993; 3: 133–134p.
17. Thompson JD, Higgins DG, Gibson TJ. ClustalW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22): 4673–4680p. Available from:
18. Jukes TH, Cantor CR. Evolution of protein molecules. In: Munro HN, editor. Mammalian Protein Metabolism. New York: Academic Press; 1969.
19. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007; 24(8): 1596–1599p. Available from:
20. Darzi MT, Ghalavand A, Rejali F. Effect of mycorrhiza, vermicompost and phosphate biofertilizer application on flowering, biological yield and root colonization in fennel (Foeniculum vulgare Mill.). Iran J Crop Sci. 2008; 10(1): 88–109p.
21. Ratti N, Kumar S, Verma HN, Gautam SP. Improvement in bioavailability of tricalcium phosphate to Cymbopo gonmartinii var. motia by rhizobacteria, AMF and Azospirillum inoculation. Microbiol Res. 2001; 156: 145–149p. Available from:
22. Shaalan MN. Effect of compost and different sources of biofertilizers, on borage plants (Borago officinalis L.). Egypt J Agric Res. 2005a; 83(1): 271–284p.
23. Shaalan MN. Influence of biofertilizers and chicken manure on growth, yield and seeds quality of (Nigella sativa, L.) plants. Egypt J Agric Res. 2005b; 83(2): 811–828p.
24. Rashmi KR, Earanna N, Vasundhara M. Influence of biofertilizers on growth, biomass and biochemical constituents of Ocimum gratissimum. L. Biomed. 2008; 3(2): 123–130p.
25. Singh S, Kapoor KK. Effects of inoculation of phosphate solubilizing microorganisms and an arbuscular mycorrhizal fungus on mung bean grown under natural soil conditions. Mycorrhiza. 1998; 7: 249–253p. Available from:
26. de Boer Sietske A, Diderichsen B. On the safety of Bacillus subtilis and B. amyloliquefaciens: a review. Appl Microbiol Biotechnol. 1991; 36: 1–4p. Available from: 10.1007/BF00164689.
27. Vaz-Moreira I, Faria C, Lopes AR, Svensson L, Falsen E, Moore ER, Ferreira ACS, Nunes OC, Manaia CM. Sphingobium vermicomposti sp. nov., isolated from vermicompost. Int J Syst Evol Microbiol. 2009; 59(12): 3145–3149p. Available from: /ijs.0.006163-0.
28. Kohler JF, Caravaca L, Carrasco, Roldan A. Interactions between a plant growth-promoting rhizobacterium, an AM fungus and a phosphate-solubilising fungus in the rhizosphere of Lactuca sativa. Appl Soil Ecol. 2007; 35: 480–487p. Available from:
29. Pandya U, Prakash S, Shende K, Dhuldhaj U, Saraf M. Multifarious allelochemicals exhibiting antifungal activity from Bacillus subtilis MBCU5. Biotech. 2017; 7(3): 175–179p. Available from:
30. Singh N, Pandey P, Dubey RC, Maheshwari DK. Biological control of root rot fungus Macrophomina phaseolina and growth enhancement of Pinus roxburghii by rhizosphere competent Bacillus subtilis BN1. World J Microbiol Biotechnol. 2008; 24: 1669–1679p. Available from:

Open Access Article
Received December 14, 2020
Accepted January 15, 2021
Published January 15, 2023