To Compare the Anti-Bacterial Activity of Uva Ursi 200 And Cantharis Vesicatoria 200 In Isolated E-Coli of Different Urine Samples by Kirby Bauer Disc Diffusion Method

Year : 2026 | Volume : 13 | 03 | Page :
By

G. Bhuvaneshwaran,

  1. CRRI, Homoeopathy (BHMS), Department of Practice of Medicine, Homoeopathy (BHMS), Excel Homoeopathy Medical College, Komarapalayam, Namakkal, Tamil Nadu, India

Abstract

Background: The most common bacterial infections are Urinary Tract Infections (UTI), primarily caused by Escherichia coli (E. coli), a gram negative bacterium responsible for 80-90% of community-acquired UTIs. The rise of antibiotic-resistant E. coli strains has increased the demand for Homoeopathic remedies. This study explores the antibacterial efficacy of Uva ursi 200 and Cantharis vesicatoria 200, using  Kirby-Bauer Disc Diffusion Method, by comparing the Zone of Inhibition (ZOI) of those remedies against E. coli to validate the traditional way of treatment in Homoeopathy. Objectives  1. To isolate and identify uropathogenic E. coli from different urine samples of UTI patients. 2. To evaluate the effectiveness of Uva ursi 200 and Cantharis Vesicatoria 200 against uropathogenic E. coli using the Kirby- Bauer Disc Diffusion Method and also to compare their antibacterial activity by paired t-statistics test. Methodology Sample collection: Midstream urine samples from 10 UTI patients were collected at Excel Homoeopathy Medical Hospital. Transport and Storage: Samples were transported within 30 minutes and stored at 4°C for upto 4 hours in Laboratory, to prevent bacterial overgrowth.  Microscopy: Presence of pus cells and bacteria on Direct Microscopic examination was examined in uncertifuged sample. Culture: E. coli cultured and confirmed using Chromocult Coliform Agar (CCA) and Sorbitol Mac-Conkey Agar (sMAC). Biochemical Tests: Indole Test and Methyl Red (MR) Test were performed. Testing Method: Kirby-Bauer Disc Diffusion applied to Muller-Hinton Agar plates. Antibacterial Agents: Uva ursi200, Cantharis vesicatoria200, Standard antibiotic (Amoxicillin) as a control and Dispensing alcohol as a negative control Measurement: Zone of Inhibition (ZOI) recorded and compared statistically using a paired t-test. Results Mean ZOI: Uva ursi200: 15.30 mm, Cantharis vesicatoria200: 12.9 mm. Statistical Analysis: Paired t-test yielded p = 0.002 (p < 0.05), indicating a significant difference. Key Findings: Uva ursi200 exhibited a larger ZOI than Cantharis vesicatoria200. Discussion Efficacy of Uva ursi200: Contains arbutin, which metabolizes into hydroquinone, exhibiting strong antibacterial properties. Efficacy of Cantharis vesicatoria200: Traditionally used for UTIs, but showed a lower ZOI compared to Uva ursi200. Homoeopathic Principles: Supports the Law of Similia and Doctrine of Drug Dynamisation, as high dilutions still demonstrated antibacterial activity. Comparison with Standard Antibiotic: The study suggests potential use of Homoeopathic remedies as alternative treatments for antibiotic-resistant UTIs. Conclusion Both Uva ursi200 and Cantharis vesicatoria200 exhibited antibacterial activity against E. coli with more significant effect in Uva ursi200. This study supports further investigation into homoeopathic treatments with larger sample sizes, clinical trials, and testing on different bacterial strains are recommended.

Keywords: Urinary Tract Infections, Escherichia coli, Uva ursi200, Cantharis vesicatoria200, Kirby-Bauer Disc Diffusion Method, Zone of Inhibition.

How to cite this article: G. Bhuvaneshwaran. To Compare the Anti-Bacterial Activity of Uva Ursi 200 And Cantharis Vesicatoria 200 In Isolated E-Coli of Different Urine Samples by Kirby Bauer Disc Diffusion Method. Research & Reviews : A Journal of Unani, Siddha and Homeopathy. 2026; 13(03):-.
How to cite this URL: G. Bhuvaneshwaran. To Compare the Anti-Bacterial Activity of Uva Ursi 200 And Cantharis Vesicatoria 200 In Isolated E-Coli of Different Urine Samples by Kirby Bauer Disc Diffusion Method. Research & Reviews : A Journal of Unani, Siddha and Homeopathy. 2026; 13(03):-. Available from: https://journals.stmjournals.com/rrjoush/article=2026/view=257643

References

  1. ^facts about E.coli: Dimensions, as discussed in Bacteria: Diversity of structure of bacteria”. Encyclopaedia Britannica, Retrived 25 June 2015.
  2. ^Fotadar u, Zaveloff p, TErracio l (2005). “Growth of Escherichia coli at elpevated temperatures”. Journal of Basic Microbiology.45(5): 403-04
  3. Al-Tawfiq JA. Increasing antibiotic resistance among isolates of Escherichia coli recovered from inpatients and outpatients in a Saudi Arabian Hospital. Infect Control Hosp Epidemiol. 2006;27:748–753.
  4. Anathanarayan and Panikers, Textbook of Microbiology 10th edition
  5. Ayatollahi, J., Fotouhi, F., Akhondimeybodi, Z., Hamidfar, M., & Shahcheraghi, S. H. (2022). Determination of antibiotic resistance of E. coli isolated from urine culture samples. The Journal of Medical Research, 8(5), 162–164. https://doi.org/10.31254/jmr.2022.8505
  6. Boericke W.Materia Medica with repertory. In Materia medica with Repertory 1927,B.Jain publishers (P)Ltd.
  7. De Paula Coelho, C., Motta, P. D., Petrillo, M., De Oliveira Iovine, R., Dalboni, L. C., Santana, F. R., Correia, M. S. F., Casarin, R. C. V., Carvalho, V. M., & Bonamin, L. V. (2017). Homeopathic medicine Cantharis modulates uropathogenic E. coli (UPEC)-induced cystitis in susceptible mice. Cytokine, 92, 103–109. https://doi.org/10.1016/j.cyto.2017.01.014
  8. Choudhri.N.M, A study of Materia Medica enriched with Real Case studies, B.Jain publishers (P) Ltd.
  9. Dromigny JA, Nabeth p, Juergens-Behr A, Perrier-Gros – Claude4 JD. Risk factors for antibiotic resistant Escherichia coli isolated from community acquired urinary tract infections in Dakar, Senegal.J Antimicrobial Chemother.2005:56:236-239.
  10. Dummer, K. J. (2022). A controlled in vitro study of the effectiveness of Withania somnifera herbal tincture and homoeopathic dilution (1X and 6X) against selected gram-positive and gram-negative bacteria. https://doi.org/10.51415/10321/2124
  11. Erb A, Sturmer T, Marre R, Brenner H.Prevalence of antibiotic resistance in Escherichia coli:overview of geographical,temporal, and methodological variations. Eur J Clin Microbial Infect Dis.2007; 26:83-90
  12. John Henry Clarke, A dictionary of Practical Materia medica – Vol I, II, and III, B.Jain publishers (P) Ltd
  13. Kahlmeter G. An international survey of the antimicrobial susceptibility of pathogens from uncomplicated urinary tract infections: the ECOSENS Project. J Antimicrobl Chemother. 2003;51:69–76.
  14. Kubitschek HE (Januuary 1990). “Cell volume increase in Escherichia coli after shifts to richer media” Journal of Bacteriology.172(1):94-101
  15. Kumar, S. S., & K, S. (2018). Activity of homoeopathic drug sulphur with different potencies on lawn culture of escherichia coli and candida albicans – topically an in-vitro evaluation. International Journal of Homoeopathic Sciences, 2(4), 23–27. https://doi.org/10.33545/26164485.2018.v2.i4a.44
  16. Kurmann, C., Imbach, E., Amsler, F., Pannek-Rademacher, S., & Pannek, J. (2018). In Vitro Effects of Homeopathic Drugs on Cultured Escherichia coli. Homeopathy, 107(02), 150–154. https://doi.org/10.1055/s-0038-1637729
  17. Kurutepe S, Surucuoglue S, Sezgin C, Gazi H, Gulay M, Ozbakkaloglu B. Increasing antimicrobial resistance in coli isolates form community-acquired urinary tract infections during 1998 – 2003 in Minisa, Turkey. Jpn J Infect Dis. 2005;58:159–161.
  18. Lockyear, H. (2021). The efficacy of a homoeopathic complex (Cantharis vesicatoris 12CH, Equisetum hyemale 12CH, Sarsaparilla 12CH, Staphisagria 12CH,Uva ursi 12CH) in the treatment of nocturnal enuresis in children between the ages of five and eighteen years, residing in children`s homes. https://doi.org/10.51415/10321/2777
  19. McKenna, J. (1998). Natural alternatives to antibiotics: Using Nature’s Pharmacy to Help Fight Infections. Penguin.
  20. Moore, M., Trill, J., Simpson, C., Webley, F., Radford, M., Stanton, L., Maishman, T., Galanopoulou, A., Flower, A., Eyles, C., Willcox, M., Hay, A., Van Der Werf, E., Gibbons, S., Lewith, G., Little, P., & Griffiths, G. (2019). Uva-ursi extract and ibuprofen as alternative treatments for uncomplicated urinary tract infection in women (ATAFUTI): a factorial randomized trial. Clinical Microbiology and Infection, 25(8), 973–980. https://doi.org/10.1016/j.cmi.2019.01.011
  21. Mukherjee, A., Aswani, M. A., & Jadhav, A. B. (2019). An in-vitro Study to Evaluate the Anti-Bacterial Activity of Rauvolfia serpentina against Escherichia coli. International Journal of Health Sciences and Research, 9(12), 39–43. https://www.ijhsr.org/IJHSR_Vol.9_Issue.12_Dec2019/5.pdf
  22. Parejo, I., Viladomat, F., Bastida, J., & Codina, C. (2001). A single extraction step in the quantitative analysis of arbutin in bearberry (Arctostaphylos uva-ursi) leaves by high-performance liquid chromatography. Phytochemical Analysis, 12(5), 336–339. https://doi.org/10.1002/pca.602
  23. Prasad, A. B., K, A., T, V., A, A. S., Sylum, S. S., V., & Cv, C. (2023). An in-vitro study of antimicrobial activity of homoeopathic medicine pyrogenium against Escherichia coli. International Journal of Homoeopathic Sciences, 7(2), 72–76. https://doi.org/10.33545/26164485.2023.v7.i2b.810
  24. Sarma, J. B. (1981). Studies on antibacterial activity of synthetic, homeopathic compounds and plant extracts on phytopathogenic bacteria in vitro.
  25. Tortora G (2010). Microbiology: An Introduction. San Francisco, CA: Benjamin Cummings.pp.85-87, 161,165.
  26. Von Baum H, Reinhard M. Antimicrobial resistance of Escherichia coli and therapeutic implications. Inter J Med Microbiol.2000:295:503-511
  27. Wagenlehner FM,Naber KG, Weidner W.Rational Antibiotic therapy of urinary tract infections. Med monatsschr Pharm.2008:31:385-390.
  28. Zhanel GG, Hisanaga TL, Laing NM, De Corby MR, Nichol KA, Weshnoweski, et al. Antibiotic resistance in Escherichia coli outpatient urinary isolates: final results from the North American Urinary Tract Infection Collaborative Alliance (NAUTICA) Int J Antimicrob Agents. 2006;27:468–475.
  29. Куркин, В. А., Рязанова, Т. К., Жестков, А. В., Лямин, А. В., Авдеева, Е. В., Куркина, А. В., Правдивцева, О. Е., & Агапов, А. И. (2018). THE ANTIBACTERIAL ACTIVITY OF COMPOUNDS FROM LEAVES OF ARCTOSTAPHYLOS UVA-URS. Chemistry of Plant Raw Material, 3, 53–60. https://doi.org/10.14258/jcprm.2018033542
Ahead of Print Subscription Original Research
Volume 13
03
Received 02/03/2026
Accepted 30/07/2026
Published 31/07/2026
Publication Time 151 Days

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