An Overview on the Characterization and Investigation of Polymerized Bio-ceramics for Bone Regenerative Applications

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Year : 2026 | Volume : 14 | 04 | Page :
By

Ravikumar Kuppusamy,

Arunnath A,

Nirmal kannan V,

Rajadurai Narayamurthy,

Dinesh Kumar S,

  1. Assistant Professor, Department of Mechanical Engineering, SRM Institute of Science and Technology Vadapalani Campus, Tamil Nadu, India
  2. Design Engineer, Department of Mechanical Engineering, Kagira Drawing Solutions, Tamil Nadu, India
  3. Professor, Department of Mechanical Engineering, Mohamed Sathak Engineering College, Tamil Nadu, India
  4. Enterprise Architect, Department of Information Technology, Tata Consultancy Services, Chennai, Tamil Nadu, India
  5. Professor, Department of Mechanical Engineering, Karpaga Vinayaga College of Engineering and Technology, Tamil Nadu, India

Abstract

Many osseous faults resulting from fracture offer inherent capacity of the body to overhaul and activate the bone grafts for recovery process. In the early development, non autologous bone grafts made of Titanium and stainless steel were suitable because of their mechanical strength, corrosion resistance, bio-compatibility and durability. Bio-degradable polymers belonging to the family of polymers and Bio-ceramics also, play a major role in orthopaedics. The polymers, poly glycolic acid, poly lactic acid, hyaluronic acid, chitosan and poly vinyl alcohol were degraded by hydrolysis, enzymatic mechanism that provides related mechanical and physical properties. The degradation characteristics were subjected to various parameters including their molecular structure, crystallinity and co-polymer ratio. Bio-ceramic materials, calcium phosphate, β tri-calcium phosphate, calcium sulphate, hydroxyapatite, tetra calcium phosphate and β calcium phosphate improves mechanical strength, bio-compatibility, wound healing process, proliferation rate and bonding strength to bone tissue which depends on the features of size of pore, shape of pore, porosity, inter-connectivity and surface area. These bio-materials were rapidly attaining identification in the domain of tissue engineering for scaffolds, extra-cellular matrix components and bone replacement for osteoporosis that can be tailored for specific patients. This review mainly focus on the investigation and characterization of polymerised bio-ceramics for the bone regenerative applications.

Keywords: Polymers, Bio-ceramics, Bio-degradable, Bio-compatibility, scaffold techniques, Bone regeneration

How to cite this article: Ravikumar Kuppusamy, Arunnath A, Nirmal kannan V, Rajadurai Narayamurthy, Dinesh Kumar S. An Overview on the Characterization and Investigation of Polymerized Bio-ceramics for Bone Regenerative Applications. Journal of Polymer & Composites. 2026; 14(04):-.
How to cite this URL: Ravikumar Kuppusamy, Arunnath A, Nirmal kannan V, Rajadurai Narayamurthy, Dinesh Kumar S. An Overview on the Characterization and Investigation of Polymerized Bio-ceramics for Bone Regenerative Applications. Journal of Polymer & Composites. 2026; 14(04):-. Available from: https://journals.stmjournals.com/jopc/article=2026/view=255078

References

1. Dorozhkin, S.V., “Calcium Orthophosphates: Applications in Nature, Biology, and Medicine”, J. Materials, [2] 399-498 (2009).
2. Yin Zhang, Jianan Ai, Dinggai Wang, Zhongrong Hong, Wenhui Li, Yoshiyuki Yokowawa, “Dissolution Properties of Different Compositions of Biphasic Calcium Phosphate Bimodal Porous Ceramics Following Immersion in Simulated Body Fluid Solution”, Cerm. Int. 39 6751-6762 (2013).
3. Hong Cao, Noboru Kuboyama, “A Biodegradable Porous Composite Scaffold of PGA/β-TCP For Bone Tissue Engineering”, J. Bone, [46] 386-395 (2010).
4. George C. East, Yimin Qin, “Wet Spinning of Chitosan and the Acetylation of Chitosan Fibers”, J. of. App. Pol. Sci., [50] 1773-1779 (1993).
5. Sadhasivam Subramaniam, Yen-Hsin Fang, Savitha Sivasubramanian, Feng-Huel Lin, Chun-Pin Lin, “Hydroxyapatite-Calcium Sulfate-Hyaluronic Acid Composite Encapsulated with Collagenase as Bone Substitute for Alveolar Bone Regeneration”, Biomaterials, [15] 00795-4 (2015).
6. Masaya Kawase, Nobuyasu Michibayashi, Yumiko Nakashima, Nobuya Kurikawa, Kiyohito Yagi, Tadashi Mioguchi, “Application of Glutaraldehyde-Crosslinked Chitosan as a Scaffold for Hepatocyte Attachment”, Biol. Pharm. Bull., 20[6] 708-710 (1997).
7. Yong-Moo Lee, Yoon-Jeong Park, Seung-Jin Lee, Young Ku, Soo-Boo Han, Sang-Mook Choi, Perry R. Klokkevold, Chong-Pyoung Chung, “Tissue Engineered Bone Formation Using Chitosan/Tricalcium Phosphate Sponges”, J Periodontal [71] 410-417 (2000).
8. Florence Croisier, Christine Jerome, “Chitosan-Based Biomaterials for Tissue Engineering”, Eur. Pol. Jor., [49] 780-792 (2013).
9. Feng Liao, Yangyang Chen, Zubing Li, Yining Wang, Bin Shi, Zhongcheng Gong, Xiangrong Cheng, “A Novel Bioactive Three-Dimensional β-Tricalcium Phosphate/Chitosan Scaffold for Periodontal Tissue Engineering”, J Mater Sci., [21] 489-496 (2010).
10. Lenka Muller, Frank A, Muller, Jurgen Zeschky, Toobias Fey, Peter Greil, “Fabrication of Hydroxyapatite Ceramics with Interconnected Macro Porosity”, Engg. Materials, 277-280 (2005).
11. Vassilis Karageorgiou, David Kalpan, “Porosity of 3D Biomaterial Scaffolds and Osteogenesis”, Biomaterials, [26] 5474-5491 (2005).
12. Peter X. MA, Ji-Won Choi, “Biodegradable Polymers Scaffolds with Well -Defined Interconnected Spherical Pore Network”, Tissue Engg., [7] (2001).
13. Sundararajan V. Madihally, Howard W.T. Matthew, “Porous Chitosan Scaffolds for Tissue Engineering”, Biomaterials, [20] 1133-1142 (1999).
14. Kalan Bastos Violin, Tamiye Simone Goia, Kunio Ishikawa, Jose Carlos Bressiani, Ana Helenda de Almeida Bressiani, “Manufacturing of Porous Ceramic Spheres Using Calcium Phosphates, by a Mechanical Method Without Additives or Binders”, Adv. In Sci. and Tech., [87] 113-117 (2014).
15. R.A.A. Muzzarelli, M. Mattioli-Belmonte, C. Tietz, R. Biagini, G. Ferioli, M.A. Brunelli, M. Fini, R. Giardino, P. Ilari, G. Biagini, “Stimulatory Effect on Bone Formation Exerted by a Modified Chitosan”, Biomaterials, [13] 1075-1081 (1994).
16. Luciano Pighinelli, Magdalena Kucharska, “Properties of Microcrystalline Chitosan-Calcium Phosphate Complex Composite”, J. of Bio. and Nanobiotechnology, [4] 20-29 (2013).
17. Jun Ping Zheng, Chuan Zeng Wang, Xiu Xing Wang, Hong Yan Wang, Hong Zhuang, Kang De Yao, “Preparation of Biomimetic Three-Dimensional Gelatin/Montmorillonite-Chitosan Scaffold for Tissue Engineering”, J. React. Func. Polym., [67] 780-788 (2007).
18. Hajime Ohgushi, Motoaki Okumura, Takafumi Yoshikawa, Keisuke Inoue, Norio Senpuku, Susumu Tamai, “Bone Formation Process in Porous Calcium Carbonate and Hydroxyapatite”, J. Biomedical Mat. Res., [26] 885-895 (1992).
19. E H J Groeneveld, E H Burger, “Bone Morphogenetic Proteins in Human Bone Regeneration”, Eur. J. of Endocrinology [142] 9-21 (2000).
20. Marshall R. Urist, Arthur Lietze, Edgar Dawson, “β-Tricalcium Phosphate Delivery System for Bone Morphogenetic Protein”, Clinical Orthopaedics and Related Research, 277-280 (1983).
21. Huipin Yuaon, J. D. De Bruijn, Yubao Li, Jianqing Feng, Zongjian Yang, K. De Groot, Xingdong Zhang, “Bone Formation Induced by Calcium Phosphate Ceramics in Soft Tissue of Dogs: A Comparative study between Porous α-TCP and β-TCP”, J. Mat. Sci., [12] 7-13 (2001).
22. Tithi Dutta Roy, Joshua L. Simon, John L. Ricci, E. Dianne Rekow, Van P. Thompson, J. Russell Parsons, “Performance of Degradable Composite Bone Repair Products Made Via Three-Dimensional Fabrication Techniques”, J. Biomed Mater Res, [66A] 283-291 (2003).
23. Pooja Bhati, Avinash Kumar, Ramya Ahuja, Naresh Bhatnagar, “Evaluating The Effect of Manufacturing Method On The Radial Compressive Force of The Bioresorbable Tubes”, J. Mat. Let., [235] 23-26 (2019).
24. Ahmed A. Haroun, Amira Gamal-Eldeen, David R. K. Harding, “Preparation, Characterization and In Vitro Biological Study of Biomimetic Three-Dimensional Gelatin-Montmorillonite/Cellulose Scaffold for Tissue Engineering”, J. Mat. Sci., [20] 2527-2540 (2009).
25. Pankaj Sarin, Sang-Jin Lee, Zlatomir D. Apostolov, Waltraud M. Kriven, “Porous Biphasic Calcium Phosphate Scaffolds from Cuttlefish Bone”, J. Am. Ceram. Soc., [8] 2362-2370 (2011).
26. G. Hotz, G. Herr, “Bone Substitute with Osteoinductive Biomaterials – Current and Future Clinical Applications”, J. Oral Maxillofac. Surg., [23] 413-417 (1994).
27. Masanobu Kamitakahara, Chikara Ohtsuki, Toshiki Miyazaki, “Behavior of Ceramic Biomaterials Derived from Tricalcium Phosphate in Physiological Condition”, J. Biomater. Appl., [23] 197-212 (2008).
28. Soon-Ho Kwon, Youn-Ki Jun, Seong-Hyeon Hong, In-Seop Lee, Hyoun-Ee Kim, Ye Yeon Won, “Calcium Phosphate Bioceramics with Various Porosities and Dissolution Rates”, J. Am. Ceram. Soc., [12] 3129-31 (2002).
29. Paul Ducheyne, Shulamith Radin, Linda King, “The Effect of Calcium Phosphate Ceramic Composition and Structure on in Vitro Behavior. I. Dissolution”, J. Biomedical Mat. Res., [27] 25-34 (1993).
30. C. P. A. T. Klein, A. A. Driessen, K. de Groot, A. ven den Hooff “Biodegradation Behavior of Various Calcium Phosphate Materials in Bone Tissue”, J. Biomed. Mat. Research, [17] 769-784 (1983).
31. Shenglei Feng, Fupo He, Jiandong Ye, “Fabrication and Characterization of Honeycomb β-Tricalcium Phosphate Scaffolds Through an Extrusion Technique”, J. Ceram. Int., [94] (2017).
32. S. Josechek, B. Nies, R. Krotz, A. Gopferich, “Chemical and Physicochemical Characterization of Porous Hydroxyapatite Ceramics Made of Natural Bone”, Biomaterials, [21] 1645-1658 (2000).
33. Fupo He, Fanwen Yang, Jixiang Zhu, Ye Peng, Xiumei Tian, Xiaoming Chen, “Fabrication of A Novel Calcium Carbonate Composite Ceramic as Bone Substitute”, J. Am. Ceram. Soc., [98] 223-228 (2015).
34. R. Emadi, S. I. Roohani Esfahani, F. Tavangarian, “A Novel, Low Temperature Method for The Preparation of β-TCP/HAP Biphasic Nanostructured Ceramic Scaffold from Natural Cancellous Bone”, Materials Letters, [64] 993-996 (2010).
35. M. Zahedi Moghadam, Sh. Hassanajili, F. Esmaeilzadeh, M. Ayatollahi, M. Ahamadi, “Formation of Porous HPCL/LPCL/HA Scaffolds with Supercritical CO2 Gas Foaming Method”, J. Mech. Beh. Biomed. Mat., [69] 115-127 (2017).
36. Dale J. Misiek, John N. Kent, Ronald F. Carr, “Soft Tissue Responses to Hydroxyapatite Particles of Different Shapes”, J. Oral. Maxilllofac Sur., [42] 150-160 (1984).
37. Tya Indah Arifta, Melvin L. Munar, Kanji Tsuru, Kunio Ishikawa, “Fabrication of Interconnected Porous Calcium – Deficient Hydroxyapatite Using the Setting Reaction of α Tricalcium Phosphate Spherical Granules”, Ceram. Int., 162-168 (2017).
38. Marc Bohner, Solene Tadier, Noemie van G Garden, Alex de Gasparo, Nicola Dobelin, Gamal Baroud, “Synthesis of Spherical Calcium Phosphate Particles for Dental and Orthopedic Apllications”. J. Biomatter, [3] 103-117 (2013).
39. Tae-Kyung Ryu, Myeong- Jin Oh, Seung-Kwan Moon, Dong-Hyun Paik, Sung-Eun Kim, Jong-Hoon Park, Sung-Wook Choi, “Uniform Tricalcium Phosphate Beads with an Open Porous Structure for Tissue Engineering”, J. Colsurfb., [112] 368-373 (2013).
40. Xiangfeng Li, Yanglong Deng, Xuening Chen, Yumei Xiao, Yujiang Fan, Xingdong Zhang, “Gelatinizing Technology Combined with Gas Foaming to Fabricate Porous Spherical Hydroxyapatite Bioceramic Granules”, J. Matlet., [185] 428-431 (2016).
41. Sergey V. Dorozhkin, “Calcium Orthophosphate Bioceramics”, J. Ceramint., [41] 13913-13966 (2015).
42. M. Simpson, B. F. Gilmore, A. Miller, J. Helt Hansen, F. Buchanan, “Irradiation of Bioresorbable Biomaterials for Controlled Surface Degradation”, J. Radphychem., [13] (2013).
43. Sandra Sanchez-Salcedoa , Daniel Arcosb , María Vallet-Regi, “Upgrading Calcium Phosphate Scaffolds for Tissue Engineering Applications”, Key Eng. Mater., [377] 19-42 (2008).
44. Shih-Ching Wu, Hsueh-Chuan Hsu, Shih-Kuang Hsu, Feng-Wei Lin, Wen-Fu Ho, “Preparation and Characterization of Porous Calcium-Phosphate Microspheres”, J. Ceramint., [41] 7596-7604 (2015).
45. S. Laasri, M. Taha, A. Hajjaji, A. Laghzizil & E.K. Hlil, “Mechanical Properties of Calcium Phosphate Biomaterials”, J. Molecular Crystals and Liquid Crystals, [628:1] 198-203 (2016).
46. Laetitia Galea, Marc Bohner, Juerg Thuering, Nicola Doebelin, Christos G. Aneziris, Thomas Graule, “Control of the Size, Shape and Composition of Highly Uniform, Non-Agglomerated, Sub-Micrometer βTricalcium Phosphate and Dicalcium Phosphate Platelets”, J. Biomaterials, [34] 6388- 6401 (2013).
47. Mark D. Timmer, Cory Carter, Catherine G. Ambrose, Antonios G. Mikos, “Fabrication of Poly (Propylene Fumarate)- based Orthopaedic Implants by Photo-crosslinking Through Transparent Silicone Molds”, J. Biomaterials, [24] 4707-4714 (2003).
48. Kuboki Y, Jin Q, Takita H, “Geometry of Carriers Controlling Phenotypic Expression in BMP-Induced Osteogenesis and Chondrogenesis”, J. Bone Joint Surg Am., [83] 105-115 (2001).
49. Jin QM, Takita H, Kohgo T, Atsumi K, Itoh H, Kuboki Y, “Effects of Geometry of Hydroxyapatite as a Cell Substratum in BMP-Induced Ectopic Bone Formation”, J. Biomed Mat Res., [45] 42-47 (1999).
50. Kuboki Y, Jin Q, Kikuchi M, Mamood J. Takita H, “Geometry of Artificial ECM: Sizes of Pores Controlling Phenotype Expression in BMP-Induced Osteogenesis and Chandrogenesis” Connect Tissue Res., [43] 529-534 (2002).
51. Chu TM, Orton DG, Hollister SJ, Feinberg SE, Halloran JW, “Mechanical and in Vivo Performance of Hydroxyapatite Implants with Controlled Architectures” Biomaterials, [23] 1283-93 (2002).
52. Kruyt MC, de Bruijin JD, Wilson CE, Oner FC, van Blitterswijk CA, Verbout AJ, et al, “Viable Osteogenic Cells are Obligatory for Tissue-Engineered Ectopic Bone Formation in Goats”, Tissue Engg., [2] 327-36 (2003).
53. Dong J, Kojima H, Uemura T, Kikuchi M, Tateishi T, Tanaka J, “In Vivo Evaluation of A Novel Porous Hydroxyapatite to Sustain Osteogenesis of Transplanted Bone Marrow-Derived Osteoblastic cells”, J. Biomed Mater Res., [57] 208-216 (2001).
54. Damien E, Hing K, Saeed S, Revell PA, “A Preliminary Study on the Enhancement of the Osteointegration of a Novel Synthetic Hydroxyapatite Scaffold in Vivo”, J. Biomed Mater Res., [66] 241-246 (2003).
55. Tsuruga E, Takita H, Itoh H, Wakisaka Y, Kuboki Y, “Pore Size of Porous Hydroxyapatite as the Cell-Substratum Controls BMP-Induced Osteogenesis”,J. Biochem. (Tokoyo), [121] 317-324 (1997).
56. Kuboki Y, Takita H, Kobayashi D, Tsuruga E, Inoue M, Murata M, et al, “BMP-Induced Osteogenesis on the Surface of Hydroxyapatite with Geometrical Feasible and Nonfeasible Structures: Topology of Osteogenesis”, J. Biomed Mater Res., [39] 190-199 (1998).
57. Yuan H, Kurashina K, de Bruijin JD, Li Y, de Groot K, Zhang X, “A Preliminary Study on Osteoinduction of Two Kinds of Calcium Phosphate Ceramics” Biomaterials, [19] 1799-1806 (1999).
58. Barralet JE, Grover L, Gaunt T, Wright AJ, Gibson IR, “Preparation of Microporous Calcium Phosphate Cement Tissue Engineering Scaffolds”, Biomaterials, [15] 3063-3072 (2002).
59. Lee YM, Seol YJ, Lim YT, Kim S, Han SB, Rhyu IC, et al., “Tissue-Engineered Growth of Bone by Marrow Cell Transplantation Using Porous Calcium Metaphosphate Matrices”, J. Biomed Mater Res., [54] 216-213 (2001).
60. Zhang C, Wang J, Feng H, Lu B, Song Z, Zhang X, “Replacement of Segmental Bone Defects using Porous Bioceramic Cylinders: A Biomechanical and X-Diffraction Study”, J. Biomed Mater Res., [54] 407-411 (2001).


Ahead of Print Subscription Review Article
Volume 14
04
Received 25/08/2026
Accepted 03/09/2026
Published 09/09/2026
Publication Time 15 Days


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