Hydrolysis-Driven Optimization of Hydroxypropyl Maize Starch for Improved Micro-Pellet Binding and Quick Solubility of Spirulina-Based Micro-Pellets

Year : 2026 | Volume : 17 | 03 | Page :
    By

    Ajay Giripunje,

  • Srikanth Billa,

  • Someshwarnath Pandey,

  • Vishnudev Gupta,

  • Bishwabhusana Palai,

  1. Senior Scientist, ACG Associated Capsules Pvt Ltd, Mumbai, Maharashtra, India
  2. Senior Scientist, ACG Associated Capsules Pvt Ltd, Mumbai, Maharashtra, India
  3. Professor, Department of Chemistry, SJJT University, Jhunjhunu, Rajasthan, India
  4. Professor, Department of Chemistry, SJJT University, Jhunjhunu, Rajasthan, India
  5. Professor, Department of Chemistry, SJJT University, Jhunjhunu, Rajasthan, India

Abstract

Acid hydrolysis is a well-established approach for modifying the physicochemical properties of polysaccharides & macromolecules including hydroxypropyl Maize starch (HPMS). In this study, the effect of varying concentrations of hydrochloric acid on the partial hydrolytic degradation of HPMS (Lycoat RS-780) was investigated at 85°C for 4 h, yielding polymers of different molecular weights. The Acid-mediated degradation effects on polymer characteristics were systematically assessed using capillary viscometry and gel permeation chromatography (GPC) to measure molecular weight and distribution. Native & Hydrolyzed Films prepared from the degraded polymers were further evaluated for glass transition temperature, tensile strength, and thermal stability. Additionally, Paracetamol-loaded microspheres based on hydroxy propylated maize starch and spirulina extract were produced from hydrolyzed polymer solutions to investigate the impact of degradation on their drug-release profile. The dissolution & disintegration results demonstrated that hydrolytic degradation substantially changed the release kinetics, confirming acid hydrolysis as an effective method for enhancing drug-release rates from HPMS-coated microspheres with spirulina extract compared with HPMC and native starch coatings.

Keywords: Hydroxy propylated Maize starch, acid hydrolysis, degradation, Spray coating, Micro-Pellet Binding.

How to cite this article:
Ajay Giripunje, Srikanth Billa, Someshwarnath Pandey, Vishnudev Gupta, Bishwabhusana Palai. Hydrolysis-Driven Optimization of Hydroxypropyl Maize Starch for Improved Micro-Pellet Binding and Quick Solubility of Spirulina-Based Micro-Pellets. Research and Reviews: A Journal of Pharmaceutical Science. 2026; 17(03):-.
How to cite this URL:
Ajay Giripunje, Srikanth Billa, Someshwarnath Pandey, Vishnudev Gupta, Bishwabhusana Palai. Hydrolysis-Driven Optimization of Hydroxypropyl Maize Starch for Improved Micro-Pellet Binding and Quick Solubility of Spirulina-Based Micro-Pellets. Research and Reviews: A Journal of Pharmaceutical Science. 2026; 17(03):-. Available from: https://journals.stmjournals.com/rrjops/article=2026/view=242571


References

1. Bourtoom T, et. al. (2008) Edible films and coatings: Characteristics and properties. International Food Research Journal, 15(3): 237–248.
2. Embuscado ME, Huber KC, et.al. (2009) Edible films and coatings for food applications. Springer, New York, NY, USA 9, 169-208.
3. Von Schantz L, Schagerof H, Nordberg Karlsson E, Ohlin M, et.al. (2014) Characterization of the substitution pattern of cellulose derivatives using carbohydrate-binding modules. BMC Biotechnology, 14(1), 113– 119.
4. El Ghzaoui A, Trompette JL, Cassanas G, Bardet L, Fabregue E, et.al. (2001) Comparative rheological behavior of some cellulosic ether derivatives. Langmuir, 17(5), 1453–1456.
5. Goodwin DJ, Picout DR, Ross-Murphya SB, Hollandc SJ, Martini LG, Lawrencea MJ, et.al. (2011) Ultrasonic degradation for molecular weight reduction of pharmaceutical cellulose ethers, Carbohydrate Polymers, 83(2),843-851.
6. Guo JH, Skinner GW, Harcum WW, Barnum PE, et.al. (1998). Pharmaceutical applications of naturally occurring water-soluble polymers. Pharmaceutical Science & Technology Today,1(6), 254–261.
7. Li J, Mei X, et.al. (2006) Applications of cellulose and cellulose derivatives in immediate release solid dosage. In RH, Marchessault F, Ravenelle, & X. X. Zhu (Eds.), Polysaccharides for drug delivery and pharmaceutical applications. ACS Symposium Series Vol. 934, (2),19-55 Washington: Oxford University Press.
8. Pfefferkorn P, Beister J, Hild A, Thielking H, Kulicke, WM, et.al. (2003). Determination of the molar mass and the radius of gyration, together with their distributions for methylhydroxyethylcellulloses. Cellulose, 10(1), 27–36.
9. Duro R, Alvarez C, Martinez-Pacheco R, Gomez-Amoza JL, Concheiro A, & Souto C, et.al. (1998) The adsorption of cellulose ethers in aqueous suspensions of pyrantel pamoate: Effects on zeta potential and stability. European Journal of Pharmaceutics and Biopharmaceutics, 45(2), 181–188.
10. Parker MD, York P, Rowe RC, et.al. (1991) Binder-substrate interactions in wet granulation.2: The effect of binder molecular weight. International Journal of Pharmaceutics, 72(3), 243–249.
11. Rowe RC, et.al. (1980) The molecular weight and molecular weight distribution of hydroxypropyl methylcellulose used in the film coating of tablets. Journal of Pharmacy and Pharmacology, 32(1), 116–119.
12. Dahl TC, Calderwood T, Bormeth A, Trimble K, Piepmeier E, et.al. (1990) Influence of physico-chemical properties of hydroxypropyl methylcellulose on naproxen release from sustained release matrix tablets. Journal of Controlled Release: Official Journal of the Controlled Release Society, 14(1), 1–10.
13. Lusvardi KM, Harcum WW, Skinner GW, Durig T, et.al (2003). Fundamentals of hydroxypropyl cellulose binders in wet granulation. AAPS Journal, 5(1744).
14. Müller RH, Keck CM, et.al. (2004) Challenges and solutions for the delivery of biotech drugs—a review of drug nanocrystal technology and lipid nanoparticles. Journal of Biotechnology, 113(1-3), 151–170.
15. Keary CM, et.al. (2001). Characterization of methocel cellulose ethers by aqueous SEC with multiple detectors. Carbohydrate Polymers, 45(3), 293–303
16. Cheng H, Wang Y, Hong Y, Wu F, Shen L, Lin X, et.al. (2024) Low viscosity hydroxypropyl methylcellulose obtained by electron beam irradiation and its performance in spray drying. International Journal of Biological Macromolecules, 275, 133626.
17. Wang Y, Xie Y, Xu D, Lin X, Feng Y, Hong Y et.al. (2014) Hydroxypropyl Methylcellulose Reduces Particle Adhesion and Improves Recovery of Herbal Extracts During Spray Drying of Chinese Herbal Medicines. Drying Technology: An International Journal, 32(5), 557-566.
18. Cheng H, Wang Y, Hong Y, Wu F, Shen L, Lin X, et.al. (2024) Low-viscosity hydroxypropyl methylcellulose obtained by electron beam irradiation and its performance in spray drying. International Journal of Biological Macromolecules, 275, 133626.
19. Wang L, Bu Y, Sun L, Chen H, et.al. (2023) A sequential combination of advanced oxidation and enzymatic hydrolysis reduces the enzymatic dosage for lignocellulose degradation. Renewable Energy, 211, 617-625.
20. Goodwin DJ, Picout DR, Ross-Murphy SB, Holland SJ, Martini LG, Lawrence MJ, et.al. (2011) Ultrasonic degradation for molecular weight reduction of pharmaceutical cellulose ethers. Carbohydrate Polymers, 83(2), 843-851.
21. Schittenhelm N, Kulicke WM, et.al. (2000) Producing homologous series of molar masses for establishing structure‐property relationships with the aid of ultrasonic degradation. Macromolecular Chemistry and Physics, 201(15), 1976-1984.
22. Camino NA, Pérez, OE, Pilosof AM, et.al. (2009) Molecular and functional modification of hydroxypropyl methylcellulose by high-intensity ultrasound. Food Hydrocolloids, 23(4), 1089-1095.
23. Demappa T, Ganesha S, Divakara S, Pattabi M, Somashekar R, et.al. (2008) Physical and thermal properties of 8 MeV electron beam irradiated HPMC polymer films. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 266(18), 3975-3980.
24. Asha S, Demappa T, Sanjeev G, Parameswara P, Somashekar R, et.al. (2009). Spectroscopic and thermal studies of 8 MeV electron beam irradiated HPMC films. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 267(14), 2385-2389.
25. Fei X, Jia W, Wang J, Chen T, Ling Y, et.al. (2020) Study on enzymatic hydrolysis efficiency and physicochemical properties of cellulose and lignocellulose after pretreatment with electron beam irradiation. International Journal of Biological Macromolecules, 145, 733-739.
26. Dow Chemicals, et.al. (1975) Handbook on methocel cellulose ether products. Dow Chemical. (d) Archer Wl, el.al. (1991) Determination of Hansen solubility parameters for selected cellulose ether derivatives. Industrial & engineering chemistry research, 30(10), 2292-2298. (Updated)
27. Yu LX et.al. (2008) Pharmaceutical quality by design: Product and process development, understanding and control. Pharmacological Research, 25(4), 781–791.
28. Andersson M, Wittgren B, Wahlund KG, et.al. (2001) Ultrahigh molar mass component detected in ethyl hydroxyethyl cellulose by asymmetrical flow field-flow fractionation coupled to multiangle light scattering. Analytical Chemistry, 73(20), 4852–4861.
29. Bos TS, Pirok BW, Karlson L, Schantz S, Dahlseid TA, Stoll DR, Somsen GW, et.al. (2024) Fingerprinting of hydroxy propyl methyl cellulose by comprehensive two-dimensional liquid chromatography-mass spectrometry of monomers resulting from acid hydrolysis. Journal of Chromatography A, 1722, 464874.
30. Sangappa B, Maheswarappa, Demappa, Thippaiah, Dasaiah, Mahadevaiah, Sanjeev, Ganesh, Divakara, Pattabi S, Manjunatha, Rudrappa, Somashekar, et.al. (2008) Physical and Thermal Properties of 8 MeV Electron Irradiated HPMC Polymer Films. Nuclear Instruments and Methods in Physics Research Section B Beam Interactions with Materials and Atoms. B 266. 3975-3980.
31. Li W, Buckton G, et.al. (2015) Using DVS-NIR to assess the water sorption behavior and stability of a griseofulvin/PVP K30 solid dispersion. Int. J. Pharm. 495(2):999–1004
32. Baghel S, Cathcart H, O’Reilly NJ, et.al (2016) Theoretical and experimental investigation of drug-polymer interaction and miscibility and its impact on drug supersaturation in aqueous medium. Eur. J. Pharm. Biopharm. 107:16–31
33. Punčochová K, Heng JY, Beránek J, Štepánek F, et.al (2014) Investigation of drug–polymer interaction in solid dispersions by vapour sorption methods. Int. J. Pharm. 469(1):159–167.
34. https://rwunwin.co.uk/wp-content/uploads/2022/06/AnyCoat-Brochure.pdf?utm_source=chatgpt.com.
35. Yoo YJ, & Um IC, et.al. (2013) Examination of thermo-gelation behavior of HPMC and HEMC aqueous solutions using rheology. Korea-Australia rheology journal, 25(2), 67-75.
36. Bodvik R, Dedinaite A, Karlson L, Bergstrom M, Baverback P, Pedersen J, Claesson PM, et.al. (2010) Aggregation and network formation of aqueous methylcellulose and hydroxypropyl methylcellulose solutions Colloids and Surfaces A: Physicochemical and Engineering Aspects, 354(1-3), 162-171.
37. Liu SQ, Joshi SC, Lam YC, Tam KC, et.al. (2008) Thermoreversible gelation of hydroxypropyl methylcellulose in simulated body fluids. Carbohyd. Polym. 72(1), 133.
38. Yoo Y J, Um IC et.al. (2013) Examination of thermo-gelation behavior of HPMC and HEMC aqueous solutions using rheology. Korea-Australia rheology journal, 25(2), 67-75.
39. Perez-Robles S, Carotenuto C, Minale M, et.al. (2022) HPMC Hydrogel Formation Mechanisms Unveiled by the Evaluation of the Activation Energy. Polymers, 14(3):635.
40. Tan YT, Heng PW, Wan LS, et.al. (1999) Mixed Polymer Coating for Modified Release from Coated Spheroids. Pharmaceutical Development and Technology, 4(4), 561-570.

Ahead of Print Subscription Original Research
Volume 17
03
Received 27/03/2026
Accepted 28/04/2026
Published 02/05/2026
Publication Time 36 Days
2

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