Expression Profiling of Immunomodulatory Genes in Canine Adipose Tissue–Derived Mesenchymal Stem Cells

Year : 2026 | Volume : 15 | 01 | Page :
    By

    Swaraj Biswas,

  1. Student, Department of Veterinary Biochemistry, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India

Abstract

Adipose tissue–derived mesenchymal stem cells (AT-MSC) represent a heterogeneous population of self-renewing, multipotent progenitor cells residing in the stromal compartment of adipose tissue. Canine adipose tissue-derived mesenchymal stem cells have attracted significant interest in regenerative medicine due to their therapeutic potential in naturally occurring canine diseases and their translational relevance to human medicine. In the present study, canine adipose tissue–derived MSCs (cAT-MSCs) were isolated and differentiated into osteogenic, chondrogenic and adipogenic lineages. Their immunomodulatory gene expression profiles were examined to evaluate their immunomodulatory capacity. cAT-MSCs were isolated and characterized by morphology, molecular profiling (CD44, CD73, CD90, CD105 positive; CD45 negative), and trilineage differentiation. Gene expression analysis demonstrated the presence of transcripts encoding vascular endothelial growth factor (VEGF), interleukin (IL)-6 and tissue inhibitors of metalloproteinases-2 (TIMP2, whereas IL-10, TGF-β, COX2, CCL2, IDO transcripts were not detected. Collectively, these findings indicate that the immunomodulatory activity of cAT-MSCs is primarily mediated through soluble factors such as IL6, VEGF and TIMP2. The results support the potential application of cAD-MSCs as a therapeutic tool for the management of immune-mediated disorders.

Keywords: Adipose tissue, Stem Cells, cAT-MSCs, morphology, molecular profiling

How to cite this article:
Swaraj Biswas. Expression Profiling of Immunomodulatory Genes in Canine Adipose Tissue–Derived Mesenchymal Stem Cells. Research and Reviews : Journal of Veterinary Science and Technology. 2026; 15(01):-.
How to cite this URL:
Swaraj Biswas. Expression Profiling of Immunomodulatory Genes in Canine Adipose Tissue–Derived Mesenchymal Stem Cells. Research and Reviews : Journal of Veterinary Science and Technology. 2026; 15(01):-. Available from: https://journals.stmjournals.com/rrjovst/article=2026/view=238868


References

  1. Amorim, R. M., Clark, K. C., Walker, N. J., Kumar, P., Herout, K., Borjesson, D. L., & Wang, A. (2020). Placenta-derived multipotent mesenchymal stromal cells: a promising potential cell-based therapy for canine inflammatory brain disease. Stem cell research & therapy, 11(1), 304.
  2. Dominici, M. L. B. K., Le Blanc, K., Mueller, I., Slaper-Cortenbach, I., Marini, F. C., Krause, D. S., … & Horwitz, E. M. (2006). Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy, 8(4), 315-317.
  3. Gugjoo, M. B., Amarpal, Fazili, M. U. R., Gayas, M. A., Ahmad, R. A., & Dhama, K. (2019). Animal mesenchymal stem cell research in cartilage regenerative medicine–a review. Veterinary Quarterly, 39(1), 95-120.
  4. Huang, Y., Wu, Q., & Tam, P. K. H. (2022). Immunomodulatory mechanisms of mesenchymal stem cells and their potential clinical applications. International Journal of Molecular Sciences, 23(17), 10023.
  5. Ivanovska, A., Wang, M., Arshaghi, T. E., Shaw, G., Alves, J., Byrne, A., … & Barry, F. (2022). Manufacturing mesenchymal stromal cells for the treatment of osteoarthritis in canine patients: challenges and recommendations. Frontiers in veterinary science, 9, 897150.
  6. Kang, S. J., Gu, N. Y., Byeon, J. S., Hyun, B. H., Lee, J., & Yang, D. K. (2023). Immunomodulatory effects of canine mesenchymal stem cells in an experimental atopic dermatitis model. Frontiers in Veterinary Science, 10, 1201382.
  7. Kotani, T., Saito, T., Suzuka, T., & Matsuda, S. (2024). Adipose-derived mesenchymal stem cell therapy for connective tissue diseases and complications. Inflammation and Regeneration, 44(1), 35.
  8. Lee, E., Lim, G. H., An, J. H., Ryu, M. O., Seo, K. W., & Youn, H. Y. (2025). Enhanced immunomodulatory effects of canine adipose tissue-derived mesenchymal stem cells in 3D culture. Frontiers in Veterinary Science, 12, 1500267.
  9. Li, J., Wu, M., & He, L. (2025). Immunomodulatory effects of mesenchymal stem cell therapy in chronic kidney disease: a literature review. BMC nephrology, 26(1), 107.
  10. Mandal, A., Jha, A. K., Biswas, D., & Guha, S. K. (2021). Derivation, characterization, and in vitro cell regeneration of canine white adipose tissue-derived mesenchymal stem cells obtained from a mesenteric region. The Journal of Basic and Applied Zoology, 82(1), 29.
  11. Russell, K. A., Chow, N. H., Dukoff, D., Gibson, T. W., LaMarre, J., Betts, D. H., & Koch, T. G. (2016). Characterization and immunomodulatory effects of canine adipose tissue-and bone marrow-derived mesenchymal stromal cells. PLoS One, 11(12), e0167442.
  12. Samsonraj, R. M., Raghunath, M., Nurcombe, V., Hui, J. H., van Wijnen, A. J., & Cool, S. M. (2017). Concise review: Multifaceted characterization of human mesenchymal stem cells for use in regenerative medicine. Stem Cells Translational Medicine, 6(12), 2173–2185
  13. Tan, Y. L., Al-Masawa, M. E., Eng, S. P., Shafiee, M. N., Law, J. X., & Ng, M. H. (2024). Therapeutic efficacy of interferon-gamma and hypoxia-primed mesenchymal stromal cells and their extracellular vesicles: underlying mechanisms and potentials in clinical translation. Biomedicines, 12(6), 1369.
  14. Tolstova, T., Dotsenko, E., Kozhin, P., Novikova, S., Zgoda, V., Rusanov, A., & Luzgina, N. (2023). The effect of TLR3 priming conditions on MSC immunosuppressive properties. Stem Cell Research & Therapy, 14(1), 344.
  15. Vizoso, F. J., Eiro, N., Cid, S., Schneider, J., & Perez-Fernandez, R. (2017). Mesenchymal stem cell secretome: Toward cell-free therapeutic strategies in regenerative medicine. International Journal of Molecular Sciences, 18(9), 1852.
  16. Ye, Y. M., Zhao, Y. X., Xiang, L. R., Zou, C. Y., Xiao, H., Lu, H., … & Xie, H. Q. (2025). The Immunomodulatory mechanism and research progress of mesenchymal stem cells in the treatment of allergic rhinitis. Stem Cell Research & Therapy, 16(1), 188.
Ahead of Print Subscription Original Research
Volume 15
01
Received 24/02/2026
Accepted 26/02/2026
Published 28/02/2026
Publication Time 4 Days
40

Login


My IP

PlumX Metrics