Recent Advancements and Comprehensive Review on Hyphenated Techniques

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nThis is an unedited manuscript accepted for publication and provided as an Article in Press for early access at the author’s request. The article will undergo copyediting, typesetting, and galley proof review before final publication. Please be aware that errors may be identified during production that could affect the content. All legal disclaimers of the journal apply.n

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Year : 2025 [if 2224 equals=””]05/10/2025 at 9:42 PM[/if 2224] | [if 1553 equals=””] Volume : 16 [else] Volume : 16[/if 1553] | [if 424 equals=”Regular Issue”]Issue : [/if 424][if 424 equals=”Special Issue”]Special Issue[/if 424] [if 424 equals=”Conference”][/if 424] 03 | Page : 39 52

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    Shreyas K. Reddy, Anjali Nayak, Devika H. G.,

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  1. Assistant Professor, Assistant Professor, Assistant Professor, Department of Quality Assurance, Krupanidhi College of Pharmacy, Bangalore, Department of Quality Assurance, Krupanidhi College of Pharmacy, Bangalore, Department of Quality Assurance, Krupanidhi College of Pharmacy, Bangalore, Karnataka, Karnataka, Karnataka, India, India, India

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nHyphenated techniques represent a powerful class of analytical methods that combine two or more established techniques – typically a separation method with a spectroscopic detection technique – to achieve enhanced analytical performance. First introduced by Hirschfeld in 1980, the term “hyphenation” refers to the online coupling of such methods, enabling more precise, sensitive, and comprehensive analysis of complex samples. These techniques exploit the strengths of individual methods while overcoming their limitations, offering superior sensitivity, selectivity, and structural insight. Hyphenated techniques are indispensable in modern analytical chemistry due to their ability to analyse intricate biological, pharmaceutical, environmental, and material samples with greater speed and accuracy. Their applications span drug development, food safety, forensic analysis, and pollutant monitoring. Common examples include GC-MS, LC-MS, LC-IR, LC-NMR, CE-MS, and ICP-MS – each with distinct advantages and drawbacks. For instance, GC-MS offers excellent separation and detection of volatile compounds but may not handle thermally labile substances. LC-NMR provides structural data but is limited by sensitivity and cost. These integrated systems address critical challenges such as limited selectivity, low sensitivity, time consumption, and destructive analysis often encountered with standalone methods. They also help overcome technique incompatibility through innovations in interface design. Ongoing developments in hyphenated technologies are driven by the need for higher throughput, automation, and real-time monitoring. Recent advancements include miniaturized systems, enhanced detectors, and software algorithms for improved data processing. These innovations are broadening the scope of hyphenated techniques, cementing their role in advancing scientific discovery across disciplines.nn

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Keywords: Hyphenation, analytical chemistry, separation techniques, spectroscopic detection, GC-MS, interface design

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nShreyas K. Reddy, Anjali Nayak, Devika H. G.. [if 2584 equals=”][226 wpautop=0 striphtml=1][else]Recent Advancements and Comprehensive Review on Hyphenated Techniques[/if 2584]. Research and Reviews: A Journal of Pharmaceutical Science. 04/10/2025; 16(03):39-52.

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nShreyas K. Reddy, Anjali Nayak, Devika H. G.. [if 2584 equals=”][226 striphtml=1][else]Recent Advancements and Comprehensive Review on Hyphenated Techniques[/if 2584]. Research and Reviews: A Journal of Pharmaceutical Science. 04/10/2025; 16(03):39-52. Available from: https://journals.stmjournals.com/rrjops/article=04/10/2025/view=0

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[if 424 not_equal=””]Regular Issue[else]Published[/if 424] Subscription Review Article

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Volume 16
[if 424 equals=”Regular Issue”]Issue[/if 424][if 424 equals=”Special Issue”]Special Issue[/if 424] [if 424 equals=”Conference”][/if 424] 03
Received 25/06/2025
Accepted 28/07/2025
Published 04/10/2025
Retracted
Publication Time 101 Days

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