Enhanced Biodiesel Production Via Encapsulation of Yeast Cells in Biocompatible Polymers

Year : 2025 | Volume : 13 | Special Issue 03 | Page : 57 62
    By

    Arti Zende,

  • Snehal Patil,

  • Jayashri Nanaware,

  • Girish Pathade,

  1. Assistant Professor, Department of Allied Sciences, Krishna Institute of Allied Sciences, Krishna Vishwa Vidyapeeth Karad, Maharshtra, India
  2. Assistant Professor, Department of Allied Sciences, Krishna Institute of Allied Sciences, Krishna Vishwa Vidyapeeth Karad, Maharshtra, India
  3. Assistant Professor, Department of Allied Sciences, Krishna Institute of Allied Sciences, Krishna Vishwa Vidyapeeth Karad, Maharshtra, India
  4. Assistant Professor, Department of Allied Sciences, Krishna Institute of Allied Sciences, Krishna Vishwa Vidyapeeth Karad, Maharshtra, India

Abstract

Yeast-based biodiesel production offers a sustainable alternative to conventional fossil fuels. However, factors like harsh environmental conditions and shear stress during processing can limit yeast cell viability and overall biodiesel yield. This study explores the potential of encapsulation technology using biocompatible polymers to improve yeast performance in biodiesel production. Encapsulation can create a protective barrier for yeast cells, shielding them from harsh environments and shear stress during processing. This protection could potentially extend yeast longevity and enhance biodiesel yield. Biocompatible polymers like alginate and chitosan are promising candidates for encapsulation due to their non-toxic nature and permeability to nutrients and substrates essential for yeast growth and biodiesel production. Additionally, encapsulation can minimize the release of yeast cells into the final biodiesel product, simplifying downstream processing. This research paves the way for further investigation into optimizing encapsulation techniques and materials for enhanced yeast performance in biodiesel production. The present study is further aimed at the isolation and screening of yeast from soil samples collected from Wing village near Karad capable of producing biodiesel. The soil sample collected was added into the enrichment medium and incubated in a rotary shaker incubator at 300C for 4-d at 180 rpm. After enrichment isolation of yeast was carried out by using the streak plate technique on potato dextrose agar. Isolated yeast colonies were screened by using the Sudan black B method (for detection of lipid granules), and isolates showing black color were used for the production of biodiesel. Extraction of lipids was carried out by using the procedure performed by Bligh and Dyer (1959) and transesterification of lipids was carried out. The yield of biodiesel obtained was 3mL/ 10 gram of yeast lipid and isolated yeast was characterized and tentatively identified as Saccharomyces cerevisiae.

Keywords: Biodiesel, yeast encapsulation, biocompatible polymers, alginate, chitosan, shear stress, biodiesel yield.

[This article belongs to Special Issue under section in Journal of Polymer and Composites (jopc)]

How to cite this article:
Arti Zende, Snehal Patil, Jayashri Nanaware, Girish Pathade. Enhanced Biodiesel Production Via Encapsulation of Yeast Cells in Biocompatible Polymers. Journal of Polymer and Composites. 2025; 13(03):57-62.
How to cite this URL:
Arti Zende, Snehal Patil, Jayashri Nanaware, Girish Pathade. Enhanced Biodiesel Production Via Encapsulation of Yeast Cells in Biocompatible Polymers. Journal of Polymer and Composites. 2025; 13(03):57-62. Available from: https://journals.stmjournals.com/jopc/article=2025/view=209487


References

  1. Rezania, Shahabaldin, Bahareh Oryani, Junboum Park, Beshare Hashemi, Krishna Kumar Yadav, Eilhann E. Kwon, Jin Hur, and Jinwoo Cho. “Review on transesterification of non-edible sources for biodiesel production with a focus on economic aspects, fuel properties and by-product applications.” Energy Conversion and Management 201 (2019): 112155.
  2. Wahlen, Bradley D., Michael R. Morgan, Alex T. McCurdy, Robert M. Willis, Michael D. Morgan, Daniel J. Dye, Bruce Bugbee, Byard D. Wood, and Lance C. Seefeldt. “Biodiesel from microalgae, yeast, and bacteria: engine performance and exhaust emissions.” Energy & Fuels 27, no. 1 (2013): 220-228.
  3. Phukan, Mayur Mausoom, Plaban Bora, Krishna Gogoi, and Bolin Kumar Konwar. “Biodiesel from Saccharomyces cerevisiae: fuel property analysis and comparative economics.” SN Applied Sciences 1 (2019): 1-10.
  4. Vasconcelos, Bruno, José Carlos Teixeira, Giuliano Dragone, and José António Teixeira. “Oleaginous yeasts for sustainable lipid production—from biodiesel to surf boards, a wide range of “green” applications.” Applied microbiology and biotechnology 103 (2019): 3651-3667.
  5. Patel, Alok, Neha Arora, Juhi Mehtani, Vikas Pruthi, and Parul A. Pruthi. “Assessment of fuel properties on the basis of fatty acid profiles of oleaginous yeast for potential biodiesel production.” Renewable and Sustainable Energy Reviews 77 (2017): 604-616.
  6. Rossi, Maddalena, Pietro Buzzini, Lisa Cordisco, Alberto Amaretti, Maurizio Sala, Stefano Raimondi, Chiara Ponzoni, Ugo Maria Pagnoni, and Diego Matteuzzi. “Growth, lipid accumulation, and fatty acid composition in obligate psychrophilic, facultative psychrophilic, and mesophilic yeasts.” FEMS microbiology ecology 69, no. 3 (2009): 363-372.
  7. Kumar, Devendra, Priyanka Nehra, Anuj Kumar, and Neeraj Kumar. “Microalgae: A Potential Source of Biofuel.” Advances in Microbial Biotechnology: Current Trends and Future Prospects (2018): 119.
  8. Sitepu, Irnayuli R., Luis A. Garay, Ryan Sestric, David Levin, David E. Block, J. Bruce German, and Kyria L. Boundy-Mills. “Oleaginous yeasts for biodiesel: current and future trends in biology and production.” Biotechnology advances 32, no. 7 (2014): 1336-1360.
  9. Gujjala, Lohit KS, SP Jeevan Kumar, Bitasta Talukdar, Archana Dash, Sanjeev Kumar, Knawang Ch Sherpa, and Rintu Banerjee. “Biodiesel from oleaginous microbes: opportunities and challenges.” Biofuels 10, no. 1 (2019): 45-59.
  10. Athenaki, Maria, Cristina Gardeli, Panagiota Diamantopoulou, Sidoine Sadjeu Tchakouteu, Dimitris Sarris, A. Philippoussis, and S. Papanikolaou. “Lipids from yeasts and fungi: physiology, production and analytical considerations.” Journal of Applied Microbiology 124, no. 2 (2018): 336-367.
  11. Song, Qingbin, Jinhui Li, and Xianlai Zeng. “Minimizing the increasing solid waste through zero waste strategy.” Journal of Cleaner Production 104 (2015): 199-210.
  12. Singh, Sangeeta, Deepshikha Pandey, Sarveshwaran Saravanabhupathy, Achlesh Daverey, Kasturi Dutta, and Kusum Arunachalam. “Liquid wastes as a renewable feedstock for yeast biodiesel production: Opportunities and challenges.” Environmental Research 207 (2022): 112100.
  13. Mota, Marta N., Paula Múgica, and Isabel Sá-Correia. “Exploring yeast diversity to produce lipid-based biofuels from agro-forestry and industrial organic residues.” Journal of Fungi 8, no. 7 (2022): 687.
  14. Zhao M, Wang Y, Zhou W, Zhou W, Gong Z. Co-valorization of crude glycerol and low-cost substrates via oleaginous yeasts to micro-biodiesel: status and outlook. Renewable and Sustainable Energy Reviews. 2023 Jul 1;180:113303.
  15. Chopra J, Rangarajan V, Sen R. Recent developments in oleaginous yeast feedstock based biorefinery for production and life cycle assessment of biofuels and value-added products. Sustainable Energy Technologies and Assessments. 2022 Oct 1;53:102621.
Special Issue Subscription Original Research
Volume 13
Special Issue 03
Received 04/02/2024
Accepted 27/12/2024
Published 29/03/2025
Publication Time 419 Days
203

Login


My IP

PlumX Metrics