Uku Eruni Philip,
Achinike Okogbule-Wonodi,
Okirie Faith Uchendu,
- Head, Department of Chemical Engineering, Federal University Otuoke, Bayelsa State, Nigeria
- Lecturer, Department of Agricultural and Environmental Engineering, Rivers State University, Port Harcourt, Rivers State, Nigeria
- Lecturer, Department of Chemical/Petrochemical Engineering, Rivers State University, Port Harcourt, Rivers State, Nigeria
Abstract
This study investigates the effect of temperature on bacterial and fungal activity in fresh and saltwater environments contaminated with crude oil. Utilizing standard characterization techniques, pure cultures of bacteria and fungi were isolated and identified. A bioreactor setup was employed to assess microbial growth and heat generation at varying temperatures (15°C to 120°C) over a 6-hour period. The results demonstrated that temperature significantly influences microbial activity, with bacterial growth rates increasing with temperature in saltwater, while fungal heat production in freshwater exhibited fluctuating patterns. Mathematical models based on Monod’s equation were developed to analyze temperature as both an inhibitor and activator of microbial growth. This research underscores the importance of temperature in bioremediation processes and microbial ecology, contributing valuable insights into managing contaminated aquatic systems.
Keywords: Temperature effect, microbial activity, bioremediation, crude oil contamination, bacterial and fungal dynamics, aquatic ecosystems
[This article belongs to Journal of Petroleum Engineering & Technology ]
Uku Eruni Philip, Achinike Okogbule-Wonodi, Okirie Faith Uchendu. Determination of Effect of Temperature on Bacteria and Fungi (Salt and Fresh Water). Journal of Petroleum Engineering & Technology. 2025; 15(01):01-08.
Uku Eruni Philip, Achinike Okogbule-Wonodi, Okirie Faith Uchendu. Determination of Effect of Temperature on Bacteria and Fungi (Salt and Fresh Water). Journal of Petroleum Engineering & Technology. 2025; 15(01):01-08. Available from: https://journals.stmjournals.com/jopet/article=2025/view=198984
References
- Abbey S. Microbial Identification and Characterization Methods. London, UK: USA: Academic Press; 1995.
- Buchanan RE, Gibbons NE. Bergey’s Manual of Determinative Bacteriology. Philadelphia, PA, USA: Williams & Wilkins; 1994.
- Cowan ST. Manual for the Identification of Medical Bacteria. Cambridge, UK: Cambridge University Press; 1974.
- Linn T. Modern Microbial Ecology. London, UK: Academic Press; 2006.
- Monod J. The Growth of Bacterial Cultures. Annu Rev Microbiol. 1949; 3: 371–3
- Meng L, Liu H, Bao M, Sun P. Microbial community structure shifts are associated with temperature, dispersants and nutrients in crude oil-contaminated seawaters. Mar Pollut Bull. 2016;111:203–12. doi: 10.1016/j.marpolbul.2016.07.010. PMID: 27485782.
- Lowe SE, Theodorou MK, Trinci AP. Growth and fermentation of an anaerobic rumen fungus on various carbon sources and effect of temperature on development. Appl Environ Microbiol. 1987;53:1210–5. doi: 10.1128/aem.53.6.1210-1215.1987. PMID: 3606103.
- Margesin R, Schinner F. Biodegradation of hydrocarbons in the environment. Microb Biotechnol. 2001; 1 (2): 178–1
- Atlas RM, Bartha R. Bioremediation: Principles and Applications. London, UK: Macmillan Press; 1993.
- Kasai Y, Yoshikawa K. Effect of temperature on bacterial community structure in oil-contaminated marine environments. FEMS Microbiol Ecol. 2006; 57 (2): 355–3
- Zeng S, Yan Z, Yang J. Stepwise algorithm and new analytical model for estimating multi-parameter of energy piles from thermal response tests. Energy Build. 2022;256:111775. doi: 10.1016/j.enbuild.2021.111775.
- Bae H, Yoon H. Modeling the growth of microorganisms in contaminated environments: a review. Environ Eng Sci. 2014; 31 (4): 208–2
- He Y, Wang H. Temperature and salinity effects on the growth of microbial communities in marine systems. Appl Microbiol Biotechnol. 2017; 101 (19): 7973–79
- Juhasz AL, Naidu R. Bioremediation of hydrocarbon-contaminated soil: a review. Environ Pollut. 2000; 107 (3): 371–3
- Leahy JG, Colwell RR. Microbial degradation of hydrocarbons in the environment. Microbiol Rev. 1990; 54 (3): 305–3

Journal of Petroleum Engineering & Technology
| Volume | 15 |
| Issue | 01 |
| Received | 19/12/2024 |
| Accepted | 12/01/2025 |
| Published | 20/01/2025 |
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