Akshita Gupta,
Rekha Karwasra,
- Research Scholar Department of Basics and Applied Sciences, Nirwan University Jaipur Rajasthan India
- Professor Department of Basics and Applied Sciences, Nirwan University Jaipur Rajasthan India
Abstract
The present study employed Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry to analyze methicillin-resistant Staphylococcus aureus peptides, focusing on various parameters associated with mass-to-charge (m/z) values. Through systematic data collection and analysis, including time, intensity, signal-to-noise ratios, quality factors, resolutions, areas under the peaks, relative intensities, full widths at half maximum, Chi-squared values, and background peaks, comprehensive insights into the spectral characteristics of methicillin-resistant Staphylococcus aureus peptides were obtained. Methicillin-resistant Staphylococcus aureus is a major public health concern due to its resistance to commonly used antibiotics and its ability to cause severe infections. The present study provided a comprehensive analysis of methicillin-resistant Staphylococcus aureus peptides using Matrix-Assisted Laser Desorption/Ionization mass spectrometry to identify potential biomarkers and therapeutic targets. Methicillin-resistant Staphylococcus aureus isolates were collected from clinical samples obtained from patients in a tertiary care hospital over a six-month period. The isolates were cultured and subjected to protein extraction procedures. Peptide profiles were generated using Matrix- Assisted Laser Desorption/Ionization mass spectrometry, and the resulting spectra were analyzed to identify specific peptides associated with methicillin-resistant Staphylococcus aureus. The analysis revealed a distinct peptide signature unique to methicillin-resistant Staphylococcus aureus isolates, differentiating them from methicillin-sensitive Staphylococcus aureus and other bacterial strains. Several peptides were identified as potential biomarkers for methicillin-resistant Staphylococcus aureus, including those involved in antibiotic resistance, virulence, and biofilm formation. Notably, peptides related to the mecA gene, responsible for methicillin resistance, were consistently detected in methicillin-resistant Staphylococcus aureus isolates. Additionally, comparative analysis of peptide profiles from different methicillin-resistant Staphylococcus aureus strains highlighted variations in protein expression related to geographic and clinical sources, providing insights into the epidemiology and evolution of methicillin-resistant Staphylococcus aureus.
Keywords: Methicillin-resistant Staphylococcus aureus, peptides, matrix-assisted laser desorption/ionization time-of-flight, mass spectrometry, antibiotic resistance.
[This article belongs to Research & Reviews: A Journal of Pharmaceutical Science(rrjops)]
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Research & Reviews: A Journal of Pharmaceutical Science
| Volume | 15 |
| Issue | 02 |
| Received | June 6, 2024 |
| Accepted | July 3, 2024 |
| Published | July 16, 2024 |