Apeksha Rathi,
Prof. Neeraj Wadhwa,
- Research Scholar Department of Biotechnology, Jaypee Institute of Information Technology, A-10 Sector-62, Noida Uttar Pradesh India
- Professor Department of Biotechnology, Jaypee Institute of Information Technology, A-10 Sector-62, Noida Uttar Pradesh India
Abstract
Mushrooms are the spore-bearing fruiting body of fungi which has a significant potential for numerous applications like pharmaceuticals, textile industry, bioremediation, soil management, alternative to toxic packaging materials and environment sustainability. Mushrooms generally belongs to the class Basidiomycota or Ascomycota. They are typically made of hyphae, the tiniest threads which comprises most fungi. Mycelia are tiny thread like structure which help in making vegetative part of fungus. In this study, the growth of mycelia from four edibles mushroom species Agaricus Bisporus, Pleurotus ostreatus, Ganoderma lucidum, Hieracium Erinaceus in Potato Dextrose Agar (PDA) and Potato Dextrose Broth (PDB) was compared. For identification of mycelia lactophenol cotton blue staining was performed. Interestingly, there was a noticeable difference in the mycelial biomass yield between (PDA) and (PDB). We report morphological characteristics of the mycelia on the basis of colour, texture, growth time requirement. There was a significant difference in yield of mycelial biomass in the (PDA) and (PDB). We could recover 1.760 gm of Pleurotus ostreatus mycelia, of 0.260 gm Agaricus Bisporus mycelia, 0.038gm of Ganoderma lucidum and 0.427 gm Hieracium Erinaceus mycelial biomass after 15 days of culturing on (PDA) agar plates. Recovery of mycelial biomass on (PDA) was better than (PDB). One of the effective methods for producing fungal biomass is to cultivate it on PDA. Our findings showed that the two media differed significantly in the mycelial biomass output, with (PDA) generally outperforming (PDB). These findings underscore the importance of cultivation medium selection in maximizing mycelial biomass production. The utilization of fungal biomass can prove to be helpful in different areas such as medicine, nutrition, agriculture, and sustainability of environment
Keywords: Mycelial growth, PDA, PDB, Agaricus Bisporus, Pleurotus ostreatus, Ganoderma lucidum, Hieracium Erinaceus
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Research & Reviews: A Journal of Microbiology & Virology
Volume | |
Received | April 19, 2024 |
Accepted | May 28, 2024 |
Published | July 3, 2024 |