International Journal of Biochemistry and Biomolecules Cover

International Journal of Biochemistry and Biomolecules

Editors Overview

ijbb maintains an Editorial Board of practicing researchers from around the world, to ensure manuscripts are handled by editors who are experts in the field of study.

Open Access
Special Issue
Topic

Mechanics of Molecules and Thermodynamics analysis of plasmid DNA

Abstract Submission Deadline : November 30, 2024

Manuscript Submission Deadline : December 25, 2024

Special Issue Description

Isothermal titration calorimetry (ITC), dynamic light scattering, surface tension measurements, and UV spectroscopy were used to examine the chemical mechanism and thermodynamics of the interactions between plasmid DNA and cationic surfactants. The cationic surfactants investigated include cetylpyridinium chloride, benzyldimethyldodecylammonium chloride, and cetyl dimethyl tetradecyl ammonium chloride. The findings point to a critical aggregation concentration (CAC) of a surfactant, below which there is no discernible interaction between DNA and surfactant. Above the CAC, the surfactant clumps with plasmid DNA. Longer hydrocarbon chains in surfactants result in smaller CAC, suggesting that hydrophobic interaction is important for DNA-surfactant complexation. Additionally, an increase in ionic strength (I) raises the critical micellization concentration (CMC) while lowering the CAC (CMC). Due to these opposing effects, a critical ionic strength (I(c)) is reached at CAC = CMC; if I(c) is lower, CAC decreases; if I(c) is higher, DNA does not form complexes with surfactant micelles. Since the CAC is constant throughout a large range of DNA concentrations, DNA in the interaction displays a pseudo-phase feature. The ITC data demonstrated that the reaction is exclusively driven by entropy because both delta H(o) and deltaS(o) have positive values, ranging from roughly 70 to 110 J K(-1) mol(-1). The combination has a DNA phosphate to surfactant molar ratio of 0.63 to 0.05. Submicrometer-sized primary particles are produced by the process, which agglomerates at high surfactant concentrations.

Keywords

Isothermal titration calorimetry, DNA, Critical micellization concentration, DNA phosphate, Plasmid DNA

Manuscript Submission information

Manuscripts should be submitted online via the manuscript Engine. Once you register on APID, click here to go to the submission form. Manuscripts can be submitted until the deadline.
All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the email address:[email protected] for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a Double-blind peer-review process. A guide for authors and other relevant information for the submission of manuscripts is available on the Instructions for Authors page.

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International Journal of Biochemistry and Biomolecules

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