Assessment of Hepatic Damage in Labeo rohita (Hamilton, 1822) Exposed to Azo dye C. I. Direct Green 6

Open Access

Year : 2023 | Volume :10 | Issue : 2 | Page : 1-

    Jagruti Barot

  1. Anita Bahadur

  1. Assistant Professor, Department of Biosciences, Veer Narmad South Gujarat University, Gujarat, India
  2. Professor, Department of Zoology, Sir P.T. Sarvajanik College of Science, Gujarat, India


Textile industries are one of the major backbones of Indian economy. They are the major reason for contamination of water bodies and their toxicity to ecosystem is an alarming environmental issue. The present study was designed to evaluate the hepatotoxic effects of C.I. Direct Green 6 (DG 6), a commercially used textile azo dye on Labeo rohita fingerlings. For this, L. rohita fingerlings were exposed to three sublethal concentrations (2.5, 5.0 and 10.0 mg/l) of DG 6 for 15 days. After 15 days of exposure period, liver tissues were dissected out and processed further for histopathological and biochemical observations. For biochemical study, liver alanine amino transferase (ALAT; E.C. and aspartate amino transferase (AST; E.C. were assayed. Histopathological result showed mild changes such as disposition of hepatic cords, hemorrhages in sinusoids with focal necrosis, depletion of cytoplasmic material from the hepatocytes, karyorrhexis etc. A marked decrease was noticed in ALAT and AST activity in the liver. Present findings revealed that DG 6 exerts toxic effects even in sublethal concentrations. Hence, histopathological and biochemical parameters of liver proved to be sensitive biomarkers to evaluate the effect of any xenobiotics such as textile or other industrial pollutants, on aquatic lives.

Keywords: Azo dye toxicity, hepatic histopathology, Labeo rohita fingerlings, alanine amino transferase (ALAT; E.C., aspartate amino transferase (AST; E.C.

[This article belongs to Research & Reviews : Journal of Veterinary Science and Technology(rrjovst)]

How to cite this article: Jagruti Barot, Anita Bahadur , Assessment of Hepatic Damage in Labeo rohita (Hamilton, 1822) Exposed to Azo dye C. I. Direct Green 6 rrjovst 2023; 10:1-
How to cite this URL: Jagruti Barot, Anita Bahadur , Assessment of Hepatic Damage in Labeo rohita (Hamilton, 1822) Exposed to Azo dye C. I. Direct Green 6 rrjovst 2023 {cited 2023 Jan 19};10:1-. Available from:

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Regular Issue Open Access Article
Volume 10
Issue 2
Received July 30, 2021
Accepted August 19, 2021
Published January 19, 2023